Animal breeding and euthanasia were performed in accordance to European Communities Council Directive 86/609/062. Grid1 KO mice [32] (gift from Jian Zuo, Memphis, TE, USA) and Grid1 -/-embryos obtained from heterozygous parents were genotyped as described [16] . Wild-type (WT) mice were purchased from Janvier Labs. All mice had C57BL/6 background.
Wild type mice
Manufactured by Janvier Labs
Sourced in France
Wild-type (WT) mice are a standard laboratory mouse model that exhibit the normal, unmodified phenotype of the species. They serve as a baseline for comparison in research studies involving genetically engineered or mutant mouse strains.
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4 protocols using wild type mice
1
Genotyping and Breeding of Grid1 Mice
2
Adult Sv129 Mice for In Vivo Experiments
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Adult Sv129 mice were used for in vivo experiments. Wild-type (wt) mice were purchased from Janvier Lab (Le Genest-St-Isle, France). Mice were maintained in cages (max. 6/cage) in a facility with 12 h light/dark cycles. Mice were fed diets ad libitum. All experiments were approved by the national ethical committee (#65352016110314184795V3).
3
Genetic Mouse Models for Cardiovascular Research
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Wildtype mice were purchased from Janvier Labs (Saint-Berthevin, France). Edg1 Cardio Cre mice (S1P1αMHCCre+ /S1P1αMHCCre−) were bred as previously published33 . SphK1- deficient mice were gifted by Richard Proia, NIH and Mfsd2b knockout mice were obtained from the MMRRC at UCDavis. Mice at the age of 12–15 weeks at the beginning of experiments were used. All animals had free access to drinking water and standard diet ad libitum.
4
Dectin-1 and Dectin-2 Knockout Mice
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Dectin-1-deficient mice (D-1KO) were kindly provided by Gordon D. Brown, and Dectin-2-deficient mice (D-2KO) were kindly provided by Mihai G. Netea; these mice were used to establish our colonies of dectin-KO mice, including Dectin-1 and Dectin-2 double-deficient mice (Dectin-1/2 deficient, D-1/2KO) and control littermates animals (wild-type—WT). These mice were generated in a C57BL/6 background and included in the protocol at 8 weeks old. Eight-week-old female wild-type mice were purchased from Janvier Laboratory (Le Genest, France) and used 1 week after reception (WTj). Animals were kept in humidity- and temperature-controlled rooms under a 12-h light-dark cycle and had access to a chow diet and water ad libitum. All experiments were performed with appropriate control groups from the same batch of mice. All experiments were performed in accordance with the ethics committee “Comite d’Ethique en Experimentation Animale” (COMETHEA C2EA – 45, Jouy en Josas, France). Every experiment was repeated at least two times n = 10.
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