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On targetplus smartpool sicontrol

Manufactured by GE Healthcare
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The ON-TARGETplus SMARTpool siControl is a laboratory equipment product designed for gene silencing experiments. It provides a pool of small interfering RNAs (siRNAs) targeting a non-coding sequence, serving as a negative control for RNA interference (RNAi) studies. The product's core function is to enable the evaluation of RNAi-mediated gene knockdown without directly targeting a specific gene of interest.

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Lab products found in correlation

Gipz lentiviral lifr and stat3 targeting human shrnas, by Thermo Fisher Scientific (2 mentions) Lipofectamine plus, by Thermo Fisher Scientific (2 mentions) Dharmafect 1 transfection reagent, by GE Healthcare (2 mentions) On targetplus smartpool sirnas for hif, by GE Healthcare (2 mentions)

Spelling variants of this product

ON-TARGETplus SMARTpool (19 citations) ON-TARGETplus (14 citations) SMARTpool (9 citations)

2 protocols using on targetplus smartpool sicontrol

1

Lentiviral Knockdown of LIFR and STAT3 in Breast Cancer

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GIPZ lentiviral LIFR- and STAT3-targeting human shRNAs were purchased from Open Biosystems (catalog numbers in parentheses). LIFR shRNA #1=V2LHS_133981 (RHS4430-200216162), shRNA #2=V2LHS_133982 (RHS4430-200175346), shRNA #3=V3LHS_347493 (RHS4430-200297260). STAT3 shRNA = V2LHS_88502 (RHS4430-200162926), V3LHS_376016 (RHS4430-200250657), V3LHS_376018 (RHS4430-200248312), V3LHS_641817 (RHS4430-200225068), V3LHS_641818 (RHS4430-200227569), V3LHS_641819 (RHS4430-200223545). GIPZ vectors were transfected into 293T cells using Lipofectamine Plus (Life Technologies) to generate lentivirus, which was then transferred onto MCF7 or SUM159 cells. Breast cancer cells were selected for 6 days in 1μg/ml puromycin and evaluated for knockdown at the protein and mRNA level. ON-TARGETplus SMARTpool siRNAs for HIF1A (L-004018-00), EPAS1 (HIF2A) (M-004814-01), SETDB1 (L-020070-00), HDAC2 (L-003495-02), and SOCS3 (L-004299-00) were purchased from Dharmacon/GE Healthcare (catalog numbers in parentheses) along with ON-TARGETplus SMARTpool siControl (D-001810-10-20) and transfected into MCF7 or SUM159 cells using DharmaFECT 1 transfection reagent (Dharmacon/GE Healthcare).
Johnson R.W., Finger E.C., Olcina M.M., Vilalta M., Aguilera T., Miao Y., Merkel A.R., Johnson J.R., Sterling J.A., Wu J.Y, & Giaccia A.J. (2016). Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow. Nature cell biology, 18(10), 1078-1089.
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2

Lentiviral Knockdown of LIFR and STAT3 in Breast Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
GIPZ lentiviral LIFR- and STAT3-targeting human shRNAs were purchased from Open Biosystems (catalog numbers in parentheses). LIFR shRNA #1=V2LHS_133981 (RHS4430-200216162), shRNA #2=V2LHS_133982 (RHS4430-200175346), shRNA #3=V3LHS_347493 (RHS4430-200297260). STAT3 shRNA = V2LHS_88502 (RHS4430-200162926), V3LHS_376016 (RHS4430-200250657), V3LHS_376018 (RHS4430-200248312), V3LHS_641817 (RHS4430-200225068), V3LHS_641818 (RHS4430-200227569), V3LHS_641819 (RHS4430-200223545). GIPZ vectors were transfected into 293T cells using Lipofectamine Plus (Life Technologies) to generate lentivirus, which was then transferred onto MCF7 or SUM159 cells. Breast cancer cells were selected for 6 days in 1μg/ml puromycin and evaluated for knockdown at the protein and mRNA level. ON-TARGETplus SMARTpool siRNAs for HIF1A (L-004018-00), EPAS1 (HIF2A) (M-004814-01), SETDB1 (L-020070-00), HDAC2 (L-003495-02), and SOCS3 (L-004299-00) were purchased from Dharmacon/GE Healthcare (catalog numbers in parentheses) along with ON-TARGETplus SMARTpool siControl (D-001810-10-20) and transfected into MCF7 or SUM159 cells using DharmaFECT 1 transfection reagent (Dharmacon/GE Healthcare).
Johnson R.W., Finger E.C., Olcina M.M., Vilalta M., Aguilera T., Miao Y., Merkel A.R., Johnson J.R., Sterling J.A., Wu J.Y, & Giaccia A.J. (2016). Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow. Nature cell biology, 18(10), 1078-1089.
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