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Tsk gel ods 80ts

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The TSK-gel ODS-80Ts is a reversed-phase high-performance liquid chromatography (HPLC) column. It is designed for the separation and analysis of a wide range of organic compounds, including pharmaceuticals, natural products, and environmental pollutants.

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Lab products found in correlation

Hawp04700, by Merck Group (2 mentions) Cordycepin, by Shimadzu (2 mentions) Lc 10avp, by Shimadzu (2 mentions) 1525 binary pump, by Waters Corporation (2 mentions) High performance liquid chromatography (hplc), by Waters Corporation (2 mentions) Tsk gel guard column, by Tosoh (2 mentions) D 14163, by Knauer (1 mentions) Agilent 1100 system, by Agilent Technologies (1 mentions) Kaempferol, by Santa Cruz Biotechnology (1 mentions) Quercetin, by Tokyo Chemical Industry (1 mentions) Gallic acid, by Merck Group (1 mentions) Spd m20a, by Shimadzu (1 mentions) 2695 separation module, by Waters Corporation (1 mentions) Waters 2487 dual λ absorbance detector, by Waters Corporation (1 mentions) Waters 2695 separation module, by Waters Corporation (1 mentions) Glycyrrhizin, by Tokyo Chemical Industry (1 mentions) Agilent hplc system, by Agilent Technologies (1 mentions) Caffeine, by Fujifilm (1 mentions) Avance 600 nmr spectrometer, by Bruker (1 mentions) Agilent 1200 series binary pump, by Agilent Technologies (1 mentions)

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ODS-80Ts (2 citations)

26 protocols using tsk gel ods 80ts

1

Aflatoxin B1 Quantification by HPLC

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Quantitation of AFB1 was carried out using HPLC (KNAUER D-14163 UV-VIS system, Germany). Briefly, 50 µl of each of the chloroformic extracts sample was injected into the HPLC C18 reverse phase column (TSKgel ODS-80TS; 4.6 mm ID × 150 mm, Tosoh Bioscience, Japan) and eluted at a flow rate of 1 ml/min by water-acetonitrile-methanol (60:25:15, v/v/v). The amount of AFB1 was measured at a wavelength of 365 nm using a fluorescence detector. A standard curve of AFB1 was plotted using various concentrations of AFB1 standard (1, 5, and 10 mg), and the amounts of AFB1 in unknown samples were calculated by comparison of the undercurved areas of the samples with authentic standards. The elution time of the samples was compared with pure AFB1 retention time (12.25 min) and quantified based on the ratio of the peak area of samples to those of the standards [ 21 (link)
].
Daliri A., Shams-Ghahfarokhi M, & Razzaghi-Abyaneh M. (2023). Detection of Aflatoxin B1-producing Aspergillus flavus strains from pistachio orchards soil in Iran by multiplex polymerase chain reaction method. Current Medical Mycology, 9(3), 1-7.
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2

Quantification of Polyphenolic Compounds

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The extract of ES was dissolved in MeOH into 0.1 mg/ml. Gallic acid (Sigma aldrich chemie GmbH, Germany), protocatechuic acid (Hwi analytik GmbH, Germany), quercetin (Tokyo Chemical Industry, Tokyo, Japan) and kaempferol (Santa Cruz Biotechnology Inc., USA) were dissolved in MeOH for analysis, either. HPLC was performed on an Agilent 1100 system (Agilent Technologies, Waldbronn, Germany) with a photodiode array detector DAD (G1315D) and Agilent 1100 series quard pump (G1311A), and an Agilent 6410 Triple Quadrupole LC/MS mass spectrometer (Agilent Technologies, Waldbronn, Germany) coupled with an ESI (electrospray ionization) interface and an ion trap mass analyzer. The ESI (electrospray ionization) source was operated in negative ionization modes. Analysis of included compounds were performed under the following conditions: column, TSK-gel ODS-80Ts (Tosoh Co., Tokyo, Japan 4.6 mm X 150 mm); mobile phase, 0.1% formic acid (solvent system A) and CH3CN (solvent system B) in a gradient mode (B from 20 to 80% in 30 min); sample injection, 5 μl; flow rate, 0.5 ml/min; temperature, 30 °C, UV wavelength, 254 nm and 350 nm. High-purity nitrogen was used as dry gas at a flow rate at 10 L/min, gas temperature at 300 °C; fragmentor voltage 150 V. Nitrogen was used as nebulizer at 30 psi and capillary voltage, ±4000 V.
Lim H.J., Jeon Y.D., Kang S.H., Shin M.K., Lee K.M., Jung S.E., Cha J.Y., Lee H.Y., Kim B.R., Hwang S.W., Lee J.H., Sugita T., Cho O., Myung H., Jin J.S, & Lee Y.M. (2018). Inhibitory effects of Euphorbia supina on Propionibacterium acnes-induced skin inflammation in vitro and in vivo. BMC Complementary and Alternative Medicine, 18, 263.
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3

Isolation and Quantification of Sucrose Esters

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Powdered
grains (50 grains) were extracted with aqueous acetone (acetone/H2O, 1:1 (v/v), 20 mL) at 25 °C for 1 day in the dark.
The aqueous acetone extracts were subjected to C18 HPLC
(TSKgel ODS-80Ts, Tosoh, 4.6 × 250 mm2; eluent, CH3CN/H2O/TFA, 5:95:0.05 to 35:65:0.05 (v/v) for 60
min by linear gradient; flow rate, 0.8 mL/min; UV detection at 320
nm) to determine 1 (tR 28.8
min), 2 (tR 44.1 min), 3 (tR 45.2 min), and 4 (tR 46.2 min).5 (link) The identity of peaks 1–4 was confirmed
with previously isolated 6-O-feruloylsucrose (1), 3′,6-di-O-sinapoylsucrose (2), 3′-O-sinapoyl-6-O-feruloylsucrose (3), and 3′,6-di-O-feruloylsucrose (4). The amounts of 1–4 were calculated using standard curves according to peak
areas.
Nakano H., Takai T, & Kondo M. (2019). Identification of Quantitative Trait Loci for the Concentrations of Phenylpropanoid Glycosides in Brown Rice. ACS Omega, 4(17), 17317-17325.
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4

HPLC Analysis of HJGE n-Butanol Extract

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The HJGE or n-butanol soluble fraction of HJGE (1 mg) was dissolved in methanol (1 mL), filtered, and analyzed with HPLC (Prominence; SHIMADZU Corporation., Kyoto, Japan). Analytical conditions were as follows: 10 μL of each sample was applied to an ODS column (TSKgel ODS-80Ts, 4.6 mm × 250 mm, 5 μm; Tosoh Corporation., Tokyo, Japan). The elution solvents were CH3CN (A) and 0.05 mol/L acetic acid-ammonium acetate buffer solution (B). After isocratic elution with 10% A and 90% B for 5 min, the elution was changed in a linear gradient to 100% A over 60 min with 10% A and 90% B. The flow rate was set at 1.0 mL/min and the column temperature at 40°C. UV spectra from 200 to 400 nm were collected with a PDA detector (SPD-M20A; SHIMADZU Corporation).
Kagawa S., Tanabe K., Hiromura M., Ogawa K., Koga T., Maeda T., Amo-Shiinoki K., Ochi H., Ichiki Y., Fukuyama S., Suzuki S., Suizu N., Ohmine T., Hamachi S., Tsuneki H., Okuya S., Sasaoka T., Tanizawa Y, & Nagashima F. (2023). Hachimijiogan, a traditional herbal medicine, modulates adipose cell function and ameliorates diet-induced obesity and insulin resistance in mice. Frontiers in Pharmacology, 14, 1167934.
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5

HPLC Analysis of Allium hookeri Extract

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Root extract of Allium hookeri was dissolved in MeOH into 50 mg/ml amd 20 ul was injected. Alliin, trans-ferulic acid, and chlorogenic acid were dissolved in MeOH into 1 mg/ml for HPLC analysis. HPLC was performed on a Waters 2695 separation module (Waters, Milford, USA) with Waters 2487 dual λ absorbance detector (Waters, Milford, USA) under following condition: column, TSK-gel ODS-80Ts (Tosoh Co., Tokyo, Japan 4.6 mmx150 mm); mobile phase, 0.1 % formic acid (solvent system A) and CH3CN (solvent system B) in a gradient mode (B 5 % from 0 to 20 min, B from 5 to 50 % from 20 to 40 min); flow rate, 0.5 ml/min; temperature, 30 °C; UV wavelength, 254 nm.
Roh S.S., Kwon O.J., Yang J.H., Kim Y.S., Lee S.H., Jin J.S., Jeon Y.D., Yokozawa T, & Kim H.J. (2016). Allium hookeri root protects oxidative stress-induced inflammatory responses and β-cell damage in pancreas of streptozotocin-induced diabetic rats. BMC Complementary and Alternative Medicine, 16, 63.
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6

Fish Freshness Assessment by K-Value

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Each fish was anesthetized with 0.03% tricaine methanesulfonate (MS-222) at 12 weeks post-hatching; heads and internal organs were removed using fine forceps and scissors under a stereomicroscope. The dressed fish were stored in a plastic container at 4 °C. A Petri dish filled with water was also placed inside the container to prevent the meat surface from drying. At intervals, the fish was taken from storage at 4 °C, and a portion (~ 10.0 mg) of the meat was homogenized with 10% perchloric acid. The homogenate was centrifuged at 15,000×g for 10 min at 4 °C. The supernatant was neutralized with 1 N KOH on ice and centrifuged at 15,000×g for 10 min at 4 °C. The supernatant was filtered through a 0.45 µm filter (Merck Millipore), and components were separated by HPLC using a column (TSK gel ODS 80Ts, 4.6 × 250 mm; Tosoh) and evaluated. Separation was achieved by increasing the acetonitrile concentration in 0.1 M NaH2PO4 (pH 4.1)46 (link). The K-value was calculated as the percentage of HxR and Hx to the sum of ATP and degradation products, as follows48 (link): K-value(%)=(HxR+Hx)/(ATP+ADP+AMP+IMP+HxR+Hx)×100
Murakami Y., Ando M., Futamata R., Horibe T., Ueda K., Kinoshita M, & Kobayashi T. (2022). Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes). Scientific Reports, 12, 18588.
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7

HPLC Analysis of Glycyrrhizin and Glycyrrhetic Acid

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GR extract lycyrrhizae was dissolved in methanol (1 mg/ml). Glycyrrhizin and glycyrrhetic acid (Tokyo Chemical Industry, Tokyo, Japan) were dissolved in methanol for high-performance liquid chromatography (HPLC) analysis. HPLC was performed on a Waters 2695 separation module (Waters, Milford, MA, USA) coupled with a model 2487 dual λ absorbance detector (Waters) under the following conditions: column, TSK-gel ODS-80Ts (4.6 mm × 150 mm; Tosoh Co., Tokyo, Japan); mobile phase, 0.1 % formic acid (solvent system A) and acetonitrile (solvent system B) in a gradient mode (B from 30 to 60 % in 15 min, B from 60 to 100 % from 15 to 30 min, B 100 % from 30 to 40 min); flow rate, 0.5 ml/min; temperature, 30 °C and ultraviolet wavelength of 254 nm.
Jeon Y.D., Bang K.S., Shin M.K., Lee J.H., Chang Y.N, & Jin J.S. (2016). Regulatory effects of glycyrrhizae radix extract on DSS-induced ulcerative colitis. BMC Complementary and Alternative Medicine, 16, 459.
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8

Quantitative Analysis of Phenethylamine

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The mass of the purified product was determined using a 3200 QTRAP MS system (Applied Biosystems, Foster City, CA, USA). The sample was directly injected to the electrospray ionization-MS detector in scan mode with positive ionization. The molecular ion peak with m/z of 122 was further analyzed by MS/MS fragmentation. The purified sample was separated using an Agilent HPLC system (Agilent) as follows: column, TSKgel ODS-80Ts (4.6 × 250 mm2, 5 μm particles; Tosoh); solvent system, A: 0.1% acetic acid in water, B: 0.1% acetic acid in acetonitrile; gradient modes: 90% A (0–5 min), 90–60% A (5–20 min), and 10% A (20–30 min); flow rate, 0.5 mL/min at 40°C. The separated sample was analyzed by LC-MS/MS, using select ion mode at m/z = 122. Standard PEA was used for comparison. The biogenic amine was confirmed as PEA by comparing its retention time and daughter ions with that of standard PEA in LC-MS/MS analysis.
Sugiyama Y., Mori Y., Nara M., Kotani Y., Nagai E., Kawada H., Kitamura M., Hirano R., Shimokawa H., Nakagawa A., Minami H., Gotoh A., Sakanaka M., Iida N., Koyanagi T., Katayama T., Okamoto S, & Kurihara S. (2022). Gut bacterial aromatic amine production: aromatic amino acid decarboxylase and its effects on peripheral serotonin production. Gut Microbes, 14(1), 2128605.
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9

Celecoxib and DM-celecoxib Plasma Quantification

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Murine blood samples were collected by cardiac puncture at the indicated times and plasma was isolated by centrifugation at 500g for 15 min. The plasma concentrations of celecoxib and DM‐celecoxib were determined by a reverse phase HPLC system (2695; Waters, Milford, MA, USA), as previously described34 with a slight modification. Briefly, the plasma samples (200 μL) containing 500 ng caffeine (Wako Pure Chemical Industries Ltd., Osaka, Japan) as an internal standard were mixed with 200 μL chloroform. After mixing, the solution was centrifuged at 13 000g for 5 min, and the organic phase was then separated and evaporated. The obtained residue was dissolved in 80 μL mobile phase (methanol:water = 72:28, v/v) and an aliquot (50 μL) was then injected into a column (TSKgel ODS‐80Ts; Tosoh, Tokyo, Japan) for separation. The running time was 10 min, and the flow rate 1.0 mL/min. Samples were measured with a UV detector operating at 254 nm. A calibration curve was prepared by plotting the ratios of celecoxib or DM‐celecoxib areas normalized to that of the internal standard.
Egashira I., Takahashi‐Yanaga F., Nishida R., Arioka M., Igawa K., Tomooka K., Nakatsu Y., Tsuzuki T., Nakabeppu Y., Kitazono T, & Sasaguri T. (2016). Celecoxib and 2,5‐dimethylcelecoxib inhibit intestinal cancer growth by suppressing the Wnt/β‐catenin signaling pathway. Cancer Science, 108(1), 108-115.
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10

Purification and Characterization of Peroxygenase Product

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After the peroxygenase reaction with OxdA(H320D) at 25°C for 30 min, the reaction mixture was extracted with ethyl acetate and concentrated by evaporation. The reaction product dissolved in water was further purified by HPLC [TSK-gel ODS-80Ts (7.8 by 300 mm; Tosoh Co.), with a linear gradient of 10~100% (v/v) acetonitrile in water]. The peak fractions were collected and concentrated to dryness (0.742 mg). Nuclear magnetic resonance (NMR) spectra of the product dissolved in CDCl3 were measured with an AVANCE-600 NMR spectrometer (Bruker, Ettlingen, Germany), and calibrated with CDCl3.
Yamada M., Hashimoto Y., Kumano T., Tsujimura S, & Kobayashi M. (2017). New function of aldoxime dehydratase: Redox catalysis and the formation of an expected product. PLoS ONE, 12(4), e0175846.
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