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Western blotting rabbit polyclonal antibodies

Manufactured by Santa Cruz Biotechnology
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Western blotting rabbit polyclonal antibodies are laboratory reagents designed for the detection and analysis of target proteins in biological samples. These antibodies specifically recognize and bind to the target protein, allowing for its visualization and quantification through the western blotting technique.

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Lab products found in correlation

Enhanced chemiluminescence detection kit, by Santa Cruz Biotechnology (2 mentions) Nitrocellulose membrane, by Santa Cruz Biotechnology (2 mentions) Mouse polyclonal antibodies, by Santa Cruz Biotechnology (2 mentions) Goat polyclonal antibodies, by Santa Cruz Biotechnology (2 mentions) Quantity one imaging analysis, by Bio-Rad (2 mentions) Beclin 1, by Santa Cruz Biotechnology (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions)

2 protocols using western blotting rabbit polyclonal antibodies

1

Western Blot Analysis of Autophagy Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The samples from cells or tissues were lysed and quantified. Twenty micrograms of protein from murine lung tissue or MH-S cells were mixed with an equal volume of 2×SDS sample buffer, boiled for 5 min and then separated by 10% SDS-polyacrylamide gel electrophoresis (PAGE). After electrophoresis, proteins were transferred to nitrocellulose membranes (Santa Cruz Biotechnology). Membranes were incubated with primary antibodies against FIP200, LC3, Beclin1, RAGE, ULK1, Histone, TLR4 and GAPDH (Santa Cruz Biotechnology). The antibodies against Atg13 and HMGB1 were from Cell Signaling Technology (Danvers, MA). Western blotting rabbit polyclonal antibodies, mouse polyclonal antibodies and goat polyclonal antibodies were obtained from Santa Cruz Biotechnology. Signals were visualized using an enhanced chemiluminescence detection kit (Santa Cruz Biotechnology). The protein signal was quantified by scanning densitometry using Quantity one imaging analysis (Bio-Rad).
Li Y., Gan C.P., Zhang S., Zhou X.K., Li X.F., Wei Y.Q., Yang J.L, & Wu M. (2014). FIP200 is Involved in Murine Pseudomonas Infection by Regulating HMGB1 Intracellular Translocation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 33(6), 1733-1744.
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2

Western Blot Analysis of Autophagy Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The samples from cells or tissues were lysed and quantified. Twenty micrograms of protein from murine lung tissue or MH-S cells were mixed with an equal volume of 2×SDS sample buffer, boiled for 5 min and then separated by 10% SDS-polyacrylamide gel electrophoresis (PAGE). After electrophoresis, proteins were transferred to nitrocellulose membranes (Santa Cruz Biotechnology). Membranes were incubated with primary antibodies against FIP200, LC3, Beclin1, RAGE, ULK1, Histone, TLR4 and GAPDH (Santa Cruz Biotechnology). The antibodies against Atg13 and HMGB1 were from Cell Signaling Technology (Danvers, MA). Western blotting rabbit polyclonal antibodies, mouse polyclonal antibodies and goat polyclonal antibodies were obtained from Santa Cruz Biotechnology. Signals were visualized using an enhanced chemiluminescence detection kit (Santa Cruz Biotechnology). The protein signal was quantified by scanning densitometry using Quantity one imaging analysis (Bio-Rad).
Li Y., Gan C.P., Zhang S., Zhou X.K., Li X.F., Wei Y.Q., Yang J.L, & Wu M. (2014). FIP200 is Involved in Murine Pseudomonas Infection by Regulating HMGB1 Intracellular Translocation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 33(6), 1733-1744.
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