Pe conjugated cd11b
PE-conjugated CD11b is a cell surface protein marker used for flow cytometry applications. It is conjugated to the fluorescent dye phycoerythrin (PE) for detection. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and granulocytes. This marker can be used to identify and characterize these cell populations.
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7 protocols using pe conjugated cd11b
Bone Marrow Cell Profiling
Cell Proliferation and Cell Cycle Analysis
Antibodies PE-conjugated CD11b were purchased from BD PharMingen (CA, USA). Total cells were Fcblocked and stained with indicated combinations of antibodies for 30 min on ice, then washed three times and resuspended in 1% FBS/PBS. For cell cycle assays, total cells were harvested and washed three times in PBS (pH 7.4), followed by fixation with precooled 70% ethanol overnight at 4 °C. Ethanol-fixed cells were centrifuged at 1000 rpm for 5 min, washed twice with PBS, and then incubated with 0.5 mL PBS containing 10 g/mL RNase A and 50 g/mL propidium iodide (PI, Sigma-Aldrich, St. Louis, MO, USA) for 30 min in the dark at 4 °C. The flow cytometric data were collected on a BD LSRFortessa flow cytometer (BD Biosciences, San Jose, CA, USA) and analyzed using FlowJo software or ModFit LT3.0 software.
Multiparameter Flow Cytometry Profiling
Multiparameter Flow Cytometry of Macrophages
Cell Surface Marker Quantification
GFP+ and mCherry+ Cell Sorting
Flow Cytometric Characterization of mBM-MSCs
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