Nci h1838

The human lung adenocarcinoma cell line HCC827 was obtained from the RIKEN BioResource Center (Tsukuba, Japan) and the NCI‐H292 and NCI‐H1838 cell lines were from the ATCC (Manassas, VA, USA). The mouse fibroblast cell line NIH3T3 was obtained from the ATCC. The human embryonic kidney cell line 293FT was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The Plat‐A retroviral packaging cell line was a kind gift from Dr Toshio Kitamura (Institute of Medical Science, University of Tokyo). HCC827, NCI‐H292 and NCI‐H1838 cells were cultured in RPMI 1640 (Nacalai Tesque, kyoto, Japan) supplemented with 10% FBS (Biowest, Nuaillé, France), 100 units/mL penicillin and 100 μg/mL streptomycin (Sigma‐Aldrich, St. Louis, MO, USA). NIH3T3 and 293FT cells were cultured in DMEM (Nacalai Tesque) supplemented with 10% FBS, 100 units/mL penicillin and 100 μg/mL streptomycin. Plat‐A cells were cultured in DMEM with 4.5 g/L glucose supplemented with 10% FBS, 100 units/mL penicillin, 100 μg/mL streptomycin, 1 μg/mL puromycin (Wako Pure Chemical Industries, Tokyo, Japan) and 10 μg/mL blasticidin (Kaken Pharmaceutical, Tokyo, Japan). Cells were maintained at 37°C in a humidified 5% CO₂ incubator (Panasonic, Osaka, Japan).