Cystatin c elisa kit

Blood samples were collected with a heparinized syringe before mice were sacrificed. Plasma creatinine level was determined using a Detect X Serum Creatinine Detection Kit (Arbor Assays, Ann Arbor, MI, USA. Cystatin C level was measured using a cystatin C Elisa kit (R&D systems, Minneapolis, MN, USA). Plasma LPO level was measured by reacting with thiobarbituric acid as described previously [34 (link)].

In the groups without ascites and with ascites but without treatment, serum samples were collected when patients presented at the hospital. In the group with ascites and treatment, serum collection was performed immediately before the administration of tolvaptan. Albumin (g/dL), total bilirubin (mg/dL), sodium (mEq/L), creatinine (mg/dL), BUN (mg/dL), CRP (mg/dL), α-fetoprotein (AFP; ng/mL) and des-γ-carboxy prothrombin (DCP; mAU/mL) were measured. Serum samples were kept at -80 °C until copeptin (pmol/L), ZAG (μg/dL), cystatin C (mg/dL), NGAL (ng/mL) and L-FABP (ng/mL) measurements were performed using an automated copeptin immunofluorescent assay kit (Thermo Fisher Scientific Inc., Tokyo, Japan), ZAG enzyme-linked immunosorbent assay (ELISA) kit (BioVendor, Brno, Czech Republic), cystatin C ELISA kit (R&D Systems, Minneapolis, MN, USA), NGAL ELISA kit (R&D Systems) and high-sensitivity human L-FABP ELISA kit (CMIC Holdings, Tokyo, Japan), respectively.
The Child-Pugh score, albumin-bilirubin (ALBI) score (18 (link)), fibrosis index based on 4 factors (FIB-4) (19 (link)), model for end-stage liver disease (MELD) score, MELD-Na score and estimated glomerular filtration rate (eGFR) with Cockcroft and Modification of Diet in Renal Disease (MDRD) formula were calculated.