Mtt reagent 5 mg ml

LPS from Escherichia coli O111:B4 (Cat no: L2630), simvastatin (Cat no: S6196), Dimethyl sulfoxide (DMSO), and Dulbecco's Phosphate Buffered Saline (D-PBS; Cat no. TL1006) were procured from Sigma-Aldrich, St. Louis, Missouri, USA. DMEM (Cat no. AL111), Fetal Bovine Serum (FBS; Cat no. RM10432), and MTT reagent (5 mg/ml) (Cat no. 4060) were procured from Himedia Laboratories Pvt. Ltd., Bengaluru, India. NF-κB p65-PE (Cat no: 558423), TNF-α–PE (Cat no. 559321), IFN-γ-PE (Cat no. 562016), and IL6-FITC (Cat no. 554544) were obtained from BD India Pvt Ltd., Bengaluru, India.

Letrozole powder (HPLC purity 99.12%) was kindly provided by Hetero drugs Pvt. Ltd., Hyderabad. DMSO (#PHR1309), camptothecin (#C9911), ethanol, berberine chloride, ascorbic acid, and gold chloride (HAuCl₄.3H₂O) were purchased from Sigma Aldrich (St. Louis, MO, USA) and used without additional purification. The MDA-MB-231 human breast adenocarcinoma cell line (NCCS, Pune) was used. The cell culture medium—high-glucose DMEM media (Cat. #: AL007)—fetal bovine serum (#RM10432), MTT reagent (5 mg/mL) (# 4060), and D-PBS (#TL1006) were purchased from Himedia, India. A 96-well plate for culturing the cells (from Corning, Bedford, MA, USA) and T25 flask (# 12556009, Biolite-Thermo, Waltham, MA, USA) were also used.