The ability of BMSCs and aBMSCs to regenerate bone was evaluated in vivo in a rat calvarial defect model as we have previously described (Kaigler, et. al. 2006 (link)) in accordance with our IACUC approved protocol (PRO00009333). Briefly, a mid-longitudinal incision was made on the dorsal surface of the cranium of RNU Nude rats (Charles River Laboratories, Mattawan, MI) prior to exposing the calvaria (n=5). A 5 mm diameter trephine bur was then used to create two circular osseous defects in the rat cranium on both sides of the sagittal suture. The full thickness of the cranial bone was removed, and collagen scaffolds with BMSCs or aBMSCs were placed in the defects. The areas were sutured with 4–0 Silk sutures and the sites allowed to heal for 8 weeks. Following 8 weeks, calvaria samples and 3-dimensional micro-CT images of the non-decalcified calvariae were captured and 3-dimensionally reconstructed. Bone mineral density (BMD) and bone volume fraction (BVF) were calculated and following this imaging, samples were decalcified and prepared histologically for standard hematoxylin and eosin (H&E) staining after which bone histomorphometry was performed as previously described.
Rnu nude rats
Manufactured by Charles River Laboratories
Sourced in United States
The RNU Nude rats are genetically engineered animals that lack a functional immune system. These rats are commonly used in biomedical research to study various diseases and test new therapies without the interference of an active immune response.
Automatically generated - may contain errors
Lab products found in correlation
Calu 3, by American Type Culture Collection (1 mentions)
Nod cg prkdcscid il2rgtm1wjl szj nsg, by Jackson ImmunoResearch (1 mentions)
Sprague dawley, by Charles River Laboratories (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
7 0 prolene suture, by Johnson & Johnson (1 mentions)
Somnosuite low flow anesthesia system, by Kent Scientific (1 mentions)
Monocrotaline mct, by Merck Group (1 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
6 protocols using rnu nude rats
1
Bone Regeneration in Rat Calvarial Defects
2
Human Lung Cancer Xenograft Model
3
Murine Comparative Study Protocols
4
Rat Myocardial Infarction Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following procedures we have described previously (67 ), male athymic rats (8 to 10 weeks old, RNU Nude rats, Charles River) were anesthetized with isoflurane, intubated, and mechanically ventilated (SomnoSuite Low-Flow Anesthesia System, Kent Scientific). Rodent Surgical Monitor (INDUS instruments) was used to monitor animal’s condition throughout the surgery, including heart rate, body temperature, and heart function through ECG. A left thoracotomy was performed to expose the heart. The pericardium was opened, and the left anterior descending coronary artery was ligated below the left atrial appendage level using a 7-0 Prolene suture (Ethicon). Occlusion was confirmed by LV blanching and ST elevation on ECG after ligation. After 1 hour, the ligation was released, the chest cavity was aseptically closed, and the rat was allowed to recover for 1 day for echocardiographic measurement. For short-term histological analysis (time points up to and including day 7), rats with I/R injuries that caused a measurable decrease in FS were deemed sufficient for injection. For long-term histological analysis (day 28) and functional recovery studies, the rats that met the echocardiographic inclusion criterion (FS < 30%) were selected for experiments and randomly assigned into experimental groups.
5
Monocrotaline-Induced Pulmonary Hypertension
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experimental procedures were approved by the institutional Animal Care Committee and performed in accordance with institutional and Canadian Council on Animal Care guidelines. Adult male Sprague-Dawley (SD) rats (Charles River, Saint Constant, QC, Canada) weighing 180–200 g were injected intraperitoneally (i.p.) with 60 mg/kg monocrotaline (MCT) (Sigma-Aldrich, St. Louis, MO, USA) or sterile water (normal controls). Adult male RNU Nude rats (Charles River, Wilmington, MA, USA) weighing 180–200 g were injected i.p. with 70 mg/kg MCT or sterile water (normal controls). Animals were provided chow and water ad libitum and housed in the institutional animal care facility until they were collected 21 days after MCT/sterile water injection. Each figure annotates relevant animal numbers used.
6
Rat Recovery and Surgery Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNU nude rats were purchased from Charles River and permitted two weeks to recover before surgery. Rats were housed individually with a 12/12 h light/dark cycle and provided food and water ad libitum. On the day of surgery, the weight of the rats was between 193 and 234 g, with a mean of 213.5 g. Procedures conformed to the recommendations of the Canadian Council on Animal Care and the National Institutes of Health guidelines for the care and use of animals, with all animal procedures performed in accordance with the University of Toronto animal care committee’s regulations. In total, nine nude rats were used for the study (N = 4 males, N = 5 females).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!