A small part of stomach tissue was excised from mice of all the groups. Those were fixed in 2.5% glutaraldehyde buffered in 0.1 M phosphate, washed in phosphate buffer thrice and osmicated in 2% osmium tetraoxide. The specimens were dried in a critical point drying apparatus (Quorum Technologies) by liquid CO2, mounted on aluminium stubs and vacuum coated with gold palladium (Polaron SC 7620). Coated specimens were then viewed in a SEM (TESCAN VEGA II L50) operated at 10 kV [59 ]. The entire specimen was scanned on a monitor at X 2.5K and 5K magnification.
Critical point drying apparatus
Manufactured by Quorum Technologies
Sourced in United Kingdom
The critical point drying apparatus is a laboratory equipment used to dry samples while preserving their structure and morphology. It operates by replacing the liquid phase of the sample with a gas phase, without passing through the liquid-gas phase transition, thereby avoiding surface tension-induced damage to the sample.
Automatically generated - may contain errors
3 protocols using critical point drying apparatus
1
Stomach Tissue Preparation for SEM
2
Scanning Electron Microscopy of Floral Bud Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pistil of FMF and BF were collected when their BVD were 3.0–5.0, 5.1–10.0, 10.1–13.0, 13.1–15, 15.1–18.0, 18.1–20.0, 21.1–25.0, and 25.1–35.0 mm (blooming). Samples were fixed in 2.5% glutaraldehyde (pH = 7.4) for > 1 week at 4°C. Following fixation, they were dehydrated using an ethanol series [30% ethanol, 20 min; 50% ethanol, 20 min; 70% ethanol, 20 min; 100% ethanol, 30 min (twice)]. The dehydrated samples were then dried in a critical-point drying apparatus (Quorum, England). Dried samples were mounted on stubs and sputter-coated with gold (FEI, America) and observed under a SEM (FEI Quanta 250, America) in Henan University. Ten floral buds were observed in each stage.
3
Scanning Electron Microscopy of Bacterial Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B. cereus NZRM5 and P. aeruginosa ATCC25668 were grown overnight in MHB and diluted with fresh MHB to achieve 1 × 107 CFU/mL. Bacterial cultures were then treated with 4 mg/mL HICA, or sterile water followed by incubation at 35°C for a total of 120 min. Samples were drawn at various treatment time intervals and the cells were harvested by centrifugation at 10,000 x g for 10 min, washed twice with 0.1 M phosphate-buffered saline (PBS) and used for SEM technique. The cells treated with sterile water served as the untreated control for comparing morphological changes after HICA treatment.
The primary fixation of cells was done with 0.1 M PBS containing 3% glutaraldehyde (Merck, Germany) for at least 8 h. The cell suspensions were passed through 0.4 μm Isopore™ membrane filters (Millipore, Ireland) to place the cells on the membrane. The membranes were washed three times with 0.1 M PBS for 15 min each. Dehydration was performed with rising ethanol concentrations as follows; 25%, 50%, 75%, 95%, and 100% for 15 min each and a final 100% for 1 h. All the samples were dried using a critical point drying apparatus (Quorum technologies, UK), mounted on the aluminium stubs and sputter coated with approximately 100 nm thickness of gold (Bal-Tec, USA). Bacteria were imaged using the FEI Quanta 200 scanning electron microscope (ThermoFisher, USA) at an accelerating voltage of 15 kV.
The primary fixation of cells was done with 0.1 M PBS containing 3% glutaraldehyde (Merck, Germany) for at least 8 h. The cell suspensions were passed through 0.4 μm Isopore™ membrane filters (Millipore, Ireland) to place the cells on the membrane. The membranes were washed three times with 0.1 M PBS for 15 min each. Dehydration was performed with rising ethanol concentrations as follows; 25%, 50%, 75%, 95%, and 100% for 15 min each and a final 100% for 1 h. All the samples were dried using a critical point drying apparatus (Quorum technologies, UK), mounted on the aluminium stubs and sputter coated with approximately 100 nm thickness of gold (Bal-Tec, USA). Bacteria were imaged using the FEI Quanta 200 scanning electron microscope (ThermoFisher, USA) at an accelerating voltage of 15 kV.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!