Tautomycin

Manufactured by Merck Group

Tautomycin is a laboratory product manufactured by Merck Group. It is a complex organic compound used as a research tool in biochemical and cell biology applications.

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Lab products found in correlation

Goat anti mouse igg hrp, by Santa Cruz Biotechnology (2 mentions) Cx 4945, by MedChemExpress (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Flag clone m2, by Merck Group (1 mentions) Gardiquimod, by InvivoGen (1 mentions) Mouse anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions) Ifnα2, by Merck Group (1 mentions) Myc clone 9e10, by Merck Group (1 mentions)

3 protocols using tautomycin

Preparation of Small Molecule Stocks

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4,5,6,7-tetrabromobenzotriazole (TBB) (Sigma), CX-4945 (MedChem Express), calyculin A (CalBiochem), and tautomycin (EMD Millipore) were dissolved in dimethylsulfoxide (DMSO) (Sigma) to create stock solutions. Control cell lines were dosed with a volume of DMSO equivalent to the volume received by the cells treated with the highest concentration of drug.

Payne J.L., Song C., Ding Y., Dhanyamraju P.K., Bamme Y., Schramm J.W., Desai D., Sharma A., Gowda C, & Dovat S. (2020). Regulation of Small GTPase Rab20 by Ikaros in B-Cell Acute Lymphoblastic Leukemia. International Journal of Molecular Sciences, 21(5), 1718.

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Protein Phosphatase Inhibitors Protocol

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Protein phosphatase inhibitors Tautomycin and LB-100 were purchased from EMD Millipore (Cat. No. 580551) and MedKoo (Cat. No. 206834), respectively. ENAN-34017 was synthesized in house as reported previously [54 (link)]. GYH-2-18 was synthesized at Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College. All the compounds were dissolved in DMSO and stored at -20°C.

Hu Z., Ban H., Zheng H., Liu M., Chang J, & Guo J.T. (2020). Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids. PLoS Pathogens, 16(7), e1008669.

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Plasmid Constructs for PP1, IRF7, and TLR7 Studies

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Flag-PP1 (α, β and γ) expression plasmids cloned in Flag-CMV4 were provided by Dr. Sherry Winter [53 (link)]. Flag-IRF7 expression plasmids and its mutants were described previously [54 , 55 (link)]. 2XMyc-IRF7 plasmids were generated by subcloning of 2XMyc-IRF7 fragment (by PCR amplification from pcDNA3-IRF7) into pCMV-Tag3B (Stratagene). TLR7 cDNA was cloned from human genome (the human B cell line BJAB) by PCR amplification. Deletion and point mutants were generated by site-directed mutation (Stratagene), and verified by sequencing. PE-conjugated IRF7(p477/479) antibody (clone K47–671) for flow cytometry was purchased from BD Biosciences. The same clone with higher concentration but without conjugation was customized for immunoblotting. Mouse anti-PP1 (clone E-9), mouse anti-PP1α (clone G-4), goat anti-PP1α (clone C19), mouse anti-IRF7 (clone G-8), rabbit anti-IRF7 (clone H-246), goat anti-mouse IgG-HRP, mouse anti-rabbit IgG-HRP, and mouse anti-goat IgG-HRP, were purchased from Santa Cruz. Flag (clone M2) and Myc (clone 9E10) antibodies were from Sigma and Roche, respectively. FITC-, PE-, APC-, and PerCP-eFluor710-conjugated antibodies for flow cytometry were purchased from eBioscience. Gardiquimod (designated as “Gardi” in figure legend) and HIV-1 ssRNA40 and controls were purchased from Invivogen. IFNα2 was purchased from Sigma. Tautomycin was purchased from EMD Millipore.

Wang L., Zhao J., Ren J., Hall K.H., Moorman J.P., Yao Z.Q, & Ning S. (2016). Protein phosphatase 1 abrogates IRF7-mediated type I IFN response in antiviral immunity. European journal of immunology, 46(10), 2409-2419.

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