SEM imaging was carried out on the uncasted 30% nHA composite groups. Samples were imaged using the FEI Quanta 200 SEM (Thermo Fisher Scientific, Waltham, MA, USA) under high vacuum mode, 5 spot size, and 25 kV accelerating voltage. To increase the contrast of the SEM images, a Fiji plugin, Enhance Local Contrast (CLAHE) filter, was applied using the following settings: block 50, bin 256, and slope 3 [24 (link)].
Fei quanta 200 sem
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FEI Quanta 200 SEM is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a field emission gun electron source, multiple detection modes, and advanced imaging capabilities, providing users with detailed information about the surface and subsurface characteristics of their samples.
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Lab products found in correlation
Scd 040, by Oerlikon Balzers (2 mentions)
Talos f200x tem, by Thermo Fisher Scientific (1 mentions)
Fei titan 80 300 tem, by Thermo Fisher Scientific (1 mentions)
Esca 3400 spectrometer, by Shimadzu (1 mentions)
Kaolin, by Tianjin Kemiou Chemical Reagent (1 mentions)
Acetone, by Tianjin Kemiou Chemical Reagent (1 mentions)
Ethanol, by Tianjin Kemiou Chemical Reagent (1 mentions)
Fecl3, by Tianjin Kemiou Chemical Reagent (1 mentions)
Hexamethyldisilazane, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
12 protocols using fei quanta 200 sem
1
SEM Imaging of 30% nHA Composites
2
In Situ Characterization of GaSb Nanowires
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The morphology, crystalline structure, and composition of the GaSb nanowires and the Ga2O3 nanotubes were characterized using the FEI Titan 80-300 TEM (ThermoFisher Scientific, Waltham, MA, USA) and the Talos F200X TEM (ThermoFisher Scientific, Waltham, MA, USA). For SEM characterization, the images were collected using the Helios 5 CX DualBeam SEM (ThermoFisher Scientific, Waltham, MA, USA) and the FEI Quanta 200 SEM (ThermoFisher Scientific, Waltham, MA, USA). Raman spectra of the GaSb nanowires and the Ga2O3 nanotubes were collected using the RAM-PRO-785E spectrometer (Agiltron, Woburn, MA, USA) with a 785 nm laser. XPS data were collected using the ESCA-3400 spectrometer (Shimadzu, Kyoto, Japan). The in situ heating experiments were performed using the Fusion 350ST heating holder combined with the micro-fabricated chips from Protochips Company, Morrisville, NC, USA. In a typical heating experiment, the GaSb nanowires were heated to a target temperature (e.g., 450 °C) with a ramp rate of 5 °C/s. Then, the temperature was increased step-by-step with a step size of 1 °C until sublimation occurred. The structure evolution was monitored via TEM or STEM mode in real-time.
3
Cryo-SEM Analysis of Plant Flowers
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Cryo-SEM was used as described (Angkawijaya et al. 2020) (link). Briefly, fresh flower samples were frozen in liquid nitrogen slush and transferred to a preparation chamber at -160°C for 5 min. Sublimation was performed at -85°C for 15 min. Then samples were coated with platinum (Pt) at -130°C, transferred to an SEM chamber and observed at -160°C using cryo-SEM (FEI Quanta 200 SEM; Thermo Fisher Scientific, Waltham, MA) with the Cryo-SEM Preparation System (PP2000TR; Quorum Technologies, Laughton, UK) at 20 kV.
4
Characterisation of ATP and M-ATP
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ATP and M-ATP samples were prepared by KBr compression and characterised by FTIR; XRD analyses were performed on an X’Pert3Powder (PANalytical Co., Heracles Almelo, The Netherlands) with graphite filter slide, a tube pressure of 40 KV, a tube current of 40 mA, 2θ diffraction angle of 50–800 and scanning step of 0.026°/s; SEM characterisation was performed by a FEI QUANTA200 SEM (FEI Co., Hillsborough, OR, USA).
5
Salivary Gland Microscopy Protocol
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TEM sections were prepared and viewed according to Guerra et al. (2015) (link). For scanning electron microscopy (SEM), flies were dissected in PBS, the salivary glands were carefully removed and placed in 80% ethanol until critical point dried by use of liquid CO2. Glands were then mounted on stubs, sputter coated with 15-nm gold and examined in an FEI Quanta 200 SEM (FEI Company, Hillsboro, OR), at 10 kV at the electron microscopy facility at Mt. Holyoke College, South Hadley, MA.
6
Scanning Electron Microscopy of Male Prolegs
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For scanning electron microscopy (SEM), the samples of male prolegs were fixed in 70% ethanol for 2 h, and then cleaned in ultrasonic bath (250 W) for 1 min in the same solution. After treatment with 100% ethanol for 30 min, the samples were dried in air. Prolegs of male were mounted on holders, and after gold-coating, the samples were examined in a FEI Quanta 200 SEM (FEI Company, the Netherlands).
7
Scanning Electron Microscopy Sample Preparation
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The parasites that were previously preserved in 5% formalin were transferred in Karnovsky’s fixative (4% paraformaldehyde and 2% glutaraldehyde in 0.1 M sodium cacodylate buffer) for 15-min, then rinsed in 0.1 M sodium cacodylate buffer two times for 20-min, before immersed in demineralized water two times for 15-min. The samples were then dehydrated through the ascending concentrations of ethanol series (20, 30, 50, 70, 90, 90, 100, and 100%) for 15-min per treatment, except 100% (30-min per treatment). Samples were subsequently dried by passing into 100% hexamethyldisilazane (HMDS) (cat. no. 52620, Fluka) for 30-min and set aside in a fume hood overnight. The dehydrated samples were attached to a strip of carbon conductive double-sided tape that was fixed to an SEM aluminum stub. Then, the samples were sputter coated with gold in Leica EM ACE200 Vacuum Coater (Leica, Wetzlar, Germany) with a thickness of 5–10 nm and examined in an FEI Quanta 200 SEM (FEI Company, Hillsboro, Oregon, United States) operating at 3–8 kV acceleration voltage, using xT Microscope Control software.
8
Biodegradable Film Disintegration in Soil
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The bio-disintegration of films in soil were studied in composting conditions, using fertile soil as substrate according to the methodology described in ASTM D5988-03 [30 ]. Film samples (3 × 3 cm2) were placed in plastic meshes and buried at 5 cm depth from the surface to ensure aerobic degradation. Previously, the films’ moisture content was determined according to Ortega et al. [18 (link),19 (link)]. The containers (120 cm3) were stored under controlled temperature and moisture conditions (20 °C and 60% RH) and daily irrigated with distilled water to maintain soil moisture. The bio-disintegration was evaluated through weight loss (%) monitoring of the samples throughout the degradation period. In all cases, extracted samples were visually inspected and photographed. Also, at the end of the assay the rest of disintegrated films were examined by scanning electron microscopy (SEM) using a FEI QUANTA 200 SEM (FEI Company, Hillsboro, OR, USA) with an Apolo 40 electron detector and acceleration voltage of 10 kV.
9
SEM Analysis of Sepiolite Penetration
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Approximately 2 × 109 CFU of stationary phase E. coli MG1655 were treated with sepiolite and friction force was applied for one minute as described for the mutagenesis experiment. Circular agar blocks were taken from agar plates with a sterile cork borer (1 cm of diameter). Then, a thin surface layer was cut off, placed on a circular glass cover slip (1.5 cm of diameter) and incubated for 45 minutes at room temperature in a laminar flow cabinet to allow air drying of the samples. The cover slips with dehydrated agar sections were mounted on aluminium stubs using double-sided adhesive tape and coated with gold in a sputter coater (SCD-040; Balzers, Union, Liechtenstein). The specimens were examined with an FEI Quanta 200 SEM (FEI Co., Hillsboro, OR) operating at an accelerating voltage of 15 kV under high vacuum mode at different magnifications. At least 5 fields from independent plates were observed to check physical penetration by the mineral. Some samples of sepiolite or asbestos (crocidolites) alone were processed and observed in the same way.
10
Fabrication of Superhydrophobic Copper Surfaces
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FEI Quanta 200 SEM (FEI company, Hillsboro, OR, USA), SL200KS contact angle meter (American Kono Industrial Co. Ltd., Seattle, WA, USA).
Copper meshes (200 meshes) were purchased from Shenyang Copper Network Co., Ltd. (Shenyang, China). Copper sheets were purchased from Tianjin Shengao Chemical Reagent Co., Ltd. (Tianjin, China). Acetone (purity > 99%), benzene (purity > 99%), ethanol (purity = 99.5%), stearic acid (SA), FeCl3 (35 wt %) (purity > 98%), H2O2 (30 wt %), and kaolin (purity > 99%) were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). SiC sandpapers (320, 400, 600, 800, 1000, 1200, and 1500 grit) were purchased from Shanghai Ruihan Vision Co., Ltd. (Shanghai, China). The experimental water was deionized water. All other chemicals were analytical-grade reagents.
Copper meshes (200 meshes) were purchased from Shenyang Copper Network Co., Ltd. (Shenyang, China). Copper sheets were purchased from Tianjin Shengao Chemical Reagent Co., Ltd. (Tianjin, China). Acetone (purity > 99%), benzene (purity > 99%), ethanol (purity = 99.5%), stearic acid (SA), FeCl3 (35 wt %) (purity > 98%), H2O2 (30 wt %), and kaolin (purity > 99%) were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). SiC sandpapers (320, 400, 600, 800, 1000, 1200, and 1500 grit) were purchased from Shanghai Ruihan Vision Co., Ltd. (Shanghai, China). The experimental water was deionized water. All other chemicals were analytical-grade reagents.
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