HSA was donated by the Chemo-Sera-Therapeutic Research Institute (Kumamoto, Japan). Prior to use, HSA was defatted with activated charcoal in an aqueous solution that had been adjusted to pH 3 with H2SO4 at 0 °C, dialyzed against de-ionized water and then freeze-dried, as originally described by Chen [25] (link). The molecular mass of HSA was assumed to be 66,500 Da. The HSA used in this study gave only one band in SDS-PAGE. Dansyl-L -asparagine (DNSA) was purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). Ibuprofen, diethylenetriaminepentaacetic acid (DTPA) and 2, 4-dinitrophenylhydrazine (DNPH) were obtained from Wako Chemical Co. (Osaka, Japan). All other chemicals were of analytical grade. All ligand molecules were first dissolved in methanol and the final concentration of methanol was less than 1% (v/v).
Dansyl l asparagine dnsa
Manufactured by Merck Group
Sourced in Sao Tome and Principe
Dansyl-L-asparagine (DNSA) is a chemical compound used as a fluorescent labeling agent in various laboratory applications. It consists of the amino acid asparagine covalently linked to the dansyl group, a fluorescent moiety. DNSA can be used to label and detect proteins, peptides, and other molecules in analytical techniques such as chromatography and electrophoresis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dansyl l asparagine dnsa
1
Defatting and Characterization of Human Serum Albumin
2
Albumin Preparation and Ligand Binding
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HSA was donated by the Chemo-Sera-Therapeutic Research Institute (Kumamoto, Japan). It was defatted with activated charcoal in an aqueous solution that had been adjusted to pH 3 with H2SO4 at 0°C, dialyzed against de-ionized water and then freeze-dried, as originally described by Chen23. The molecular mass of HSA was assumed to be 66,500 Da. The HSA used in this study gave only one band in SDS-PAGE. Dansyl-L-asparagine (DNSA) was purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). Ibuprofen and sodium oleate (Ole) were obtained from Wako Chemical Co. (Osaka, Japan). All other chemicals were of analytical grade. All buffers used were prepared with sodium phosphate dibasic and sodium phosphate monobasic salts. All ligand molecules were first dissolved in methanol; the final concentration of methanol was less than 1% (v/v).
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