The concentrations of glucose, urea, uric acid, creatinine, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transpeptidase (GGT) activities were determined by the modular autoanalyzer (Roche, Germany). The NF-κB levels were measured in the obtained serum samples using the commercial Rat NF-κB ELISA Kit (Catalog Number: E-EL-R0673, Elabscience, China). The serum 8-OHdG (oxidative DNA damage) level was determined using a commercial kit (Enzo Life Science Company of DNA Damage) ELISA kit (Catalog Number: ADI-EKS-350). The levels of glycosylated hemoglobin (HbA1c) were analyzed in whole blood using a commercial kit (Roche, Germany) and an autoanalyzer (Roche Cobas Integra 800) on the same day.
Rat nf κb elisa kit
Manufactured by Elabscience
Sourced in China
The Rat NF-κB ELISA Kit is a laboratory product designed for the quantitative determination of NF-κB levels in rat samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique.
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2 protocols using rat nf κb elisa kit
1
Comprehensive Biomarker Assessment Protocol
2
Quantification of TNF-α, PGE2, and NFκB
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TNF-α, PGE2, and NFκB expressions were measured using the Rat TNF-α ELISA kit (catalog no: E-EL-R0019), Rat PGE2 ELISA kit (catalog no: E-EL-0034), and Rat NFκB ELISA kit (catalog no: E-EL-R0674), respectively, in accordance with the manufacturer’s guidelines (Elabscience). Using the Silent Crusher M (Heidolph) apparatus, the tissues were homogenized at 15,000 RPM; after centrifugation, the supernatant was collected (for 1 h at 1,350 g). TNF-α, PGE2, and p-NFκB concentrations were measured using an ELISA microplate reader. (Ansari et al., 2019 (link)).
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