Cell viability was measured by Cck-8 assay. FMECs and GCs cells were cultured in 96-well plates treated with different concentrations (25, 50, 100, 200, 400 μg/mL) of AS for 24 h. Then, liquid in the plates was changed into complete medium without AS. Cck-8 (Solarbio) was added to each well and incubated for 2 h at 37°C. The absorbance at 450 nm was measured by full wavelength microplate reader (Bio Tek, Vermont, USA).
Full wavelength microplate reader
Manufactured by Agilent Technologies
Sourced in United States
The Full-wavelength microplate reader is a laboratory instrument designed to measure the absorbance of samples in microplates across a wide range of wavelengths. It is capable of detecting and quantifying various analytes in solution.
Automatically generated - may contain errors
Lab products found in correlation
Cck 8, by Solarbio (2 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
P aminobenzoic acid, by Merck Group (1 mentions)
Hypoxanthine thymidine medium, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Solarbio (1 mentions)
7 protocols using full wavelength microplate reader
1
Cell Viability Evaluation by CCK-8 Assay
2
Immunoassay Development for Quinoxaline Derivatives
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Quinocetone (QCT), OLA, MEQ, CBX, cyadox (CYX), DMEQ, desoxyolaquindox (DOLA), desoxyquinocatone (DQCT), desoxycarbadox (DCBX), desoxycyadox (DCYX), N1-desoxycyadox (N1-DCYX), N4-desoxycyadox (N4-DCYX), MQCA, and QCA were purchased from Sigma (USA). N-acetylsulfanilyl chloride, p-aminobenzoic acid (PABA), carboxymethyl hydroxylamine (AOAA), N-hydroxysuccinimide, pyridine, bovine serumalbumin (BSA), human serum albumin (HSA), dimethyl sulfoxide (DMSO), ovalbumin (OVA), polyethylene glycol (PEG), hypoxanthine–aminopterin–thymidine (HAT) medium, hypoxanthine–thymidine (HT) medium, and peroxidase-labelled goat anti-mouse immunoglobulins (HRP-IgG) were purchased from Sigma-Aldrich (USA). Australian fetal bovine serum was purchased from Thermo Fisher Bioengineering Materials (Beijing, China). The SP2/0 mouse-tumor cell line was obtained from our laboratory. All other chemicals and organic solvents were of analytical grade or better. Female Balb/c mice (6–8 weeks old, NO. 42000600000485) were bought from Three Gorges University (Yichang, China) and quality-tested by the Hubei Provincial Center for Disease Control and Prevention (Wuhan, China).
A full-wavelength microplate reader (BioTek, Winooski, VT, USA) and a UV spectrophotometer (Model 8453) were purchased from Agilent Technologies, Inc. (Santa Clara, CA, USA).
A full-wavelength microplate reader (BioTek, Winooski, VT, USA) and a UV spectrophotometer (Model 8453) were purchased from Agilent Technologies, Inc. (Santa Clara, CA, USA).
3
Mesenchymal Stem Cell Proliferation on Scaffolds
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The 5th generation purified rat bone marrow-derived mesenchymal stem cells were cultured were seeded on the BCP scaffolds at a concentration of 3 × 105 per scaffold in a-Dulbecco's modified Eagle's medium (a-MEM, Gibco, USA) supplemented with 10% fetal goat serum (Excell, China) and 1% penicillin/streptomycin (Gibco, USA) in a humidified atmosphere of 5% CO2 at 37 °C. at days 1, 3, 7 and 10, the proliferation of BMSCs on scaffolds was analyzed by the Alamar Blue reagent (Invitrigon, USA) cells were incubated with 10% Alamar Blue reagent (5 mL Alamar Blue in 45 mL medium) for 2 h at 37 °C. Next, the media were transferred to a 96-well plate used for measuring the optical density (OD) at 570 nm and 600 nm using a full-wavelength microplate reader (BioTek, USA). Each experiment was carried out in triplicate.
4
MTT Assay for Cell Viability
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LLC triple-mutant cells and H1975 cells were seeded at 1 × 104 cells per well in 96-well plates for 24 h. Then, cells were treated with different concentrations of FYLM-containing serum. After 48 h of intervention, 100 µl medium containing 20% MTT (5 mg/ml, Solarbio, Beijing, China) was added to each well. After incubation at 37°C for 4 h, 150 µl dimethylsulfoxide (DMSO) was added to each well and shaken on a shaker at low speed for 10 min to dissolve the formazan crystals. In the end, the absorbance values of each well were measured at 490 nm (Lin et al., 2015 (link)) using a full-wavelength microplate reader (BioTek, United States).
5
Evaluating Effects of Recombinant Proteins on HCC Cell Viability
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The effects of rPK5, rGal-3C, and rPK5-RL-Gal-3C on HCC cell viability were measured with a MTT assay kit (VICMED, Xuzhou, China). Briefly, tumor cells were planted on 96-well plates. Next day, the medium was replaced with new medium containing purified rPK5, rGal-3C, or rPK5-RL-Gal-3C under the indicated concentrations (0, 1, 2, 3 and 4 μmol/l), and then incubated for further 48 h. Following added MTT reagent and incubated for 4 h, the absorbance was read at 570 nm with a full wavelength microplate reader (BioTek Instruments, VT, USA).
6
CX Cytotoxicity Assay on FMECs and GCs
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FMECs and GCs cells were cultured in 96-well plates treated with different concentrations (25, 50, 100, 200, 400 μg/mL) of CX for 24 h or 48 h. Removed the medium and cck-8 (Solarbio, Beijing, China) was added to each well and incubated for 2 h at 37℃. Used full wavelength microplate reader (Bio Tek, Vermont) measure the absorbance at 450 nm.
7
Cadmium-Induced Cell Viability Assay
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Cell viability measurement was performed as previously reported [38 (link)]. Briefly, the rat proximal tubule cells in the logarithmic growth phase in 96-well plates were treated with different concentrations of Cd for 12 h, and this was repeated six times within each group. In addition, blank control wells (with culture medium only) were set up. After Cd treatment, 10 μL of CCK-8 reagent was added to each well, which was shaken and mixed gently and then incubated in the cell incubator in the dark for 1–2 h. OD values were determined using a full-wavelength microplate reader (BioTek, Winooski, VT, USA) at a wavelength of 450 nm. The cell viability was calculated according to the formula [(OD treatment group-OD blank group/OD control-OD blank group)] × 100%.
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