Noradrenaline

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Noradrenaline is a laboratory reagent used for research purposes. It is a catecholamine neurotransmitter that plays a role in the sympathetic nervous system. Noradrenaline is used in various scientific studies, including those related to neuroscience and biochemistry.

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Noradrenaline bitartrate (2 citations)

5 protocols using noradrenaline

Pharmacological Agents in Tissue Assays

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Resversatrol, noradrenaline, tyramine, isoproterenol, IBMX, lidocaine and diltiazem were obtained from Tocris Bioscience (Madrid, Spain) and dimethyl sulphoxide (DMSO) from Probus, (Barcelona, Spain).
Resveratrol and IBMX were dissolved in DMSO and Tyrode solution (20% DMSO in Tyrode) and noradrenaline, tyramine, isoproterenol, lidocaine and diltiazem were dissolved in Tyrode solution. This stock solution was diluted in a pre-warmed and pre-aerated bathing solution to achieve the final concentration desired. The drug was added to the organ bath at an appropriate concentration so that the concentration of DMSO in the test solution was less than 0.3%, which did not affect any of the preparations.

, & Hernandez-Cascales J. (2017). Resveratrol enhances the inotropic effect but inhibits the proarrhythmic effect of sympathomimetic agents in rat myocardium. PeerJ, 5, e3113.

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Evaluation of Cellular Metabolism Modulators

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All reagents were purchased from ThermoFisher (Waltham, MA, USA) unless otherwise stated. TOFA (5-(tetradecyloxy)-2-furoic acid^{13 (link)}; ab141578) and Tolbutamide were purchased from Abcam; Noradrenaline from Tocris Bioscience (Bristol, UK); 2-Bromopalmitate from Sigma-Aldrich; Tetrodotoxin, ω-agatoxin and isradipine from Alomone Labs (Jerusalem, Israel).

Veprik A., Denwood G., Liu D., Bany Bakar R., Morfin V., McHugh K., Tebeka N.N., Vetterli L., Yonova-Doing E., Gribble F., Reimann F., Hoehn K.L., Hemsley P.A., Ahnfelt-Rønne J., Rorsman P., Zhang Q., de Wet H, & Cantley J. (2022). Acetyl-CoA-carboxylase 1 (ACC1) plays a critical role in glucagon secretion. Communications Biology, 5, 238.

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Pharmacological Reagents for Neuroscience Research

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MK‐801, NASPM, NBQX, noradrenaline, and prazosin were obtained from Tocris. All other reagents were from Sigma‐Aldrich.

Copeland D.S., Gugel A, & Gantz S.C. (2023). Potentiation of neuronal activity by tonic GluD1 current in brain slices. EMBO Reports, 24(7), e56801.

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Modulation of Ca2+ Signaling Pathways

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The following substances were used (source given in parentheses): the L-type Ca²⁺ channel blocker isradipine and the P/Q Ca²⁺ channel blocker ω-agatoxin (Alomone Labs; Jerusalem, Israel); the α₁-antagonist prazosin (Abcam, Cambridge, UK); the membrane-permeable PKA inhibitor myr-PKI, the IP₃ receptor inhibitor Xestospongin C, the NAADP antagonist Ned-19, the V-ATPase inhibitor bafilomycin, the sER ATPase inhibitor thapsigargin and noradrenaline (Tocris Bioscience, Bristol, UK); the EPAC2 inhibitor ESI-05 (BioLog, Bremen, Germany); the insulin receptor antagonist (S961, Novo-Nordisk, Denmark). All other compounds were obtained from Sigma-Aldrich (Dorset, UK).

Hamilton A., Zhang Q., Salehi A., Willems M., Knudsen J.G., Stephen S., Ringgaard A.K., Chapman C.E., Gonzalez-Alvarez A., Surdo N.C., Zaccolo M., Basco D., Johnson P.R., Ramracheya R., Rutter G.A., Galione A., Rorsman P, & Tarasov A.I. (2018). Adrenaline stimulates glucagon secretion by Tpc2-dependent Ca2+ mobilization from acidic stores in pancreatic α-cells. Diabetes, 67(6), 1128-1139.

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Characterization of phosphodiesterase 3A

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, cilostamide, rolipram, noradrenaline and ouabain were obtained from Tocris Bioscience (Bristol, UK) whereas desmethylimipramine and phentolamine were from Sigma-Aldrich (Madrid, Spain). cAMP, 5'-AMP and 5'-nucleotidase from Crotalus atrox venom were products from Enzo Life Sciences (Farmingdale, NY, USA). Pierce ® BCA protein assay kit was from Thermo Fisher Scientific (Rockford, IL, USA) and BioGel P-6DG for gel filtration was from Bio-Rad (Hercules, CA, USA). Polyvinylidene difluoride Amersham Hybond P 0.45 membrane for electroblotting was from GE Healthcare (Madrid, Spain). Rabbit antibodies anti-human PDE3A (sc-20792) and anti-rat ERK (sc-154) were from Santa Cruz Biotechnology (Heidelberg, Germany) and secondary antibody anti-rabbit IgG conjugated to horseradish peroxidase (W4011) was from Promega Biotech Ibérica (Madrid, Spain). All other reagents for the biochemical procedures were analytical grade and provided by Sigma-Aldrich (Madrid, Spain).

Soler F., Fernández-Belda F., Pérez-Schindler J, & Hernández-Cascales J. (2015). Single inhibition of either PDE3 or PDE4 unmasks β2-adrenoceptor-mediated inotropic and lusitropic effects in the left but not right ventricular myocardium of rat. European journal of pharmacology, 765.

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