Transwell plates with 0.4 μm pore sized inserts

Manufactured by Corning

Transwell plates with 0.4-μm pore sized inserts are a type of cell culture plate designed for various in vitro experiments. The inserts feature a semi-permeable membrane with a pore size of 0.4 micrometers, which allows for the study of cell migration, permeability, and co-culture applications.

Automatically generated - may contain errors

Lab products found in correlation

Fitc labeled dextran, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

0.4 μm Transwell plates (0.4-μm pore, Transwell 0.4 μm Transwell inserts Pore transwells Transwell plates

2 protocols using transwell plates with 0.4 μm pore sized inserts

Permeability Assay for Endothelial Barrier

Check if the same lab product or an alternative is used in the 5 most similar protocols

HUVECs, IMR90-4 derived Epi-iBMECs, and rECs were cultured as a monolayer onto Transwell plates with 0.4-μm pore sized inserts (Corning). Cells were grown till confluent at which time 50 ng/mL anti-VE-cadherin antibody (BV9) or 100 ng/mL VEGF-A was added to the cell culture media. Seventy kilodaltons of rhodamine-labeled dextran (Invitrogen) was then pipetted in each well and allowed to incubate for 6 h. Media passed through the Transwell filters were then collected and analyzed on a spectrophotometer (Bio-Rad) measuring the amount of dextran allowed to pass through the cell monolayers at each condition.

Lu T.M., Houghton S., Magdeldin T., Durán J.G., Minotti A.P., Snead A., Sproul A., Nguyen D.H., Xiang J., Fine H.A., Rosenwaks Z., Studer L., Rafii S., Agalliu D., Redmond D, & Lis R. (2021). Pluripotent stem cell-derived epithelium misidentified as brain microvascular endothelium requires ETS factors to acquire vascular fate. Proceedings of the National Academy of Sciences of the United States of America, 118(8), e2016950118.

+ Open protocol

+ Expand

Quantifying Endothelial Barrier Integrity

Check if the same lab product or an alternative is used in the 5 most similar protocols

CTRL and SCZ iBMECs were passaged and cultured as a monolayer (100,000 cells/well) onto Transwell plates with 0.4-μm pore sized inserts (Corning). Cells were grown until confluency at which time 50 ng/mL anti-VE-cadherin antibody (BV9) was added to the cell culture media. Seventy kilodaltons of FITC-labeled dextran (Invitrogen) was then pipetted into each well and allowed to incubate for 6 h. The amount of dextran that passed through the cell monolayer was measured by collecting the media passed through the Transwell filter and analyzing it on a spectrophotometer (Bio-Rad). dextran intensity graphs were created using Prism.

Stankovic I., Notaras M., Wolujewicz P., Lu T., Lis R., Ross M.E, & Colak D. (2024). Schizophrenia endothelial cells exhibit higher permeability and altered angiogenesis patterns in patient-derived organoids. Translational Psychiatry, 14, 53.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!