Column chromatography (CC) were conducted with Silica gel (200–300 mesh, Qingdao Marine Chemical, Inc.) and Lichroprep RP-18 (40–63 μm, Merck). Waters 2695 liquid chromatography system was applied to run Semipreparative HPLC with a YMC-Pack 10 mm × 250 mm column (Pro C18 RS). Thin-layer chromatography was carried out on precoated TLC plates (200–250 μm thickness, Silica gel 60 F254, Qingdao Marine Chemical, Inc.). Bruker DRX-500 and Avance III-600 MHz spectrometers (Bruker, Zűrich, Switzerland) were used to record 1D and 2D NMR data with solvent signal as internal reference. ESIMS, HREIMS and HRESIMS were obtained from a Shimadzu LCMS-IT-TOF MS (Shimadzu, Kyoto, Japan), a Waters AutoSpec Premier P776 MS (Waters Corporation, Milford, USA) or an Agilent G6230 TOF MS (Agilent Technologies, Palo Alto, USA). Shimadzu IR-450 instrument was used to evaluate infrared spectra with KBr pellets. A JASCO P-1020 digital polarimeter was applied to test optical rotations, using MeOH as solvent. Quantitative-PCR (q-PCR) was conducted on ProFlex™ PCR system (Thermo Fisher, Shanghai, China).
Ir 450 instrument
Manufactured by Shimadzu
The IR-450 is a Fourier Transform Infrared (FTIR) spectrometer manufactured by Shimadzu. It is designed to analyze the infrared absorption spectrum of samples, providing information about their molecular composition and structure.
Automatically generated - may contain errors
Lab products found in correlation
P 1020 digital polarimeter, by Jasco (3 mentions)
Drx 500, by Bruker (3 mentions)
Lichroprep rp 18, by Merck Group (3 mentions)
Agilent g6230 tof ms, by Agilent Technologies (2 mentions)
Proflex pcr system, by Thermo Fisher Scientific (2 mentions)
Silica gel, by Qingdao Marine Chemical (2 mentions)
Silica gel 60 f254, by Qingdao Marine Chemical (2 mentions)
2695 liquid chromatography system, by Waters Corporation (1 mentions)
Avance 3 600 mhz spectrometer, by Bruker (1 mentions)
Avance 3 600 mhz, by Bruker (1 mentions)
Mini protean tetra electrophoresis system, by Bio-Rad (1 mentions)
G6230 tof ms, by Agilent Technologies (1 mentions)
Smart apex ccd crystallography system, by Bruker (1 mentions)
Avance 3 600, by Bruker (1 mentions)
Lcms it tof mass spectrometer, by Shimadzu (1 mentions)
3 protocols using ir 450 instrument
1
Comprehensive Analytical Techniques for Natural Product Characterization
2
Physicochemical Characterization of Compounds
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A JASCO P-1020 digital polarimeter was applied to record optical rotations, using MeOH as solvent. 1D and 2D NMR spectra were performed on Bruker DRX-500 and Avance III-600 MHz spectrometers (Bruker, Zűrich, Switzerland) with solvent signal as internal reference. ESIMS and HRESIMS were run on an Agilent G6230 TOF MS (Agilent Technologies, Palo Alto, USA). Infrared spectra were tested on a Shimadzu IR-450 instrument with KBr pellets. Column chromatography (CC) was run on Silica gel (200–300 mesh, Qingdao Marine Chemical, Inc.), and Lichroprep RP-18 (40–63 μm, Merck). Semipreparative HPLC was carried out on an Agilent 1100 liquid chromatography system using an YMC-Pack 10 mm × 250 mm column (Pro C18 RS). Precoated TLC plates (200–250 μm thickness, Silica gel 60 F254, Qingdao Marine Chemical, Inc.) were used for thin-layer chromatography. The spots in TLC were visualized by heating after spraying with 10% aq. H2SO4.
3
Chromatography and Spectroscopy Techniques
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Column chromatography (CC) was run on silica gel (200–300 mesh, Qingdao Marine Chemical, Inc.) and Lichroprep RP-18 (40–63 μm, Merck). Semipreparative HPLC was carried out on an Agilent 1100 liquid chromatography system using a YMC-Pack 10 mm × 250 mm column (Pro C18 RS). Precoated TLC plates (200–250 μm thickness, silica gel 60 F254, Qingdao Marine Chemical, Inc.) were used for thin-layer chromatography. 1D and 2D NMR spectra were obtained using Bruker DRX-500 and Avance III-600 MHz spectrometers (Bruker, Zűrich, Switzerland) with solvent signal as internal reference. ESIMS and HRESIMS were run with a Shimadzu LCMS-IT-TOF mass spectrometer (Shimadzu, Kyoto, Japan) or an Agilent G6230 TOF MS (Agilent Technologies, Palo Alto, USA). Infrared spectra were obtained using a Shimadzu IR-450 instrument with KBr pellets. A JASCO P-1020 digital polarimeter was applied to record optical rotations, using MeOH as solvent. X-ray diffraction was realized with a Bruker SMART APEX CCD crystallography system. Flow cytometry analysis was conducted with a PARTEC brand flow cytometer (Germany). Quantitative PCR (q-PCR) was conducted with a ProFlex™ PCR system (Thermo Fisher, Shanghai, China). SDS-PAGE was carried out with a Mini-Protean Tetra Electrophoresis System (Bio-Rad, Shanghai, China).
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