Milli q uf filtration system

Navitoclax and the internal standard, navitoclax-d8, were purchased from Toronto Research Chemicals (Toronto, ON). HPLC grade acetonitrile and formic acid were purchased from EMD Chemical Inc. (Billerica, MA). Deionized water was obtained from the Millipore Milli-Q-UF filtration system (Milford, MA). All other chemicals were of molecular biological grade or higher and were obtained from Sigma–Aldrich (St. Louis, MO). Drug-free sodium heparin human plasma was purchased from Biological Specialty Company (Colmar, PA, USA).

Terameprocol (purity of 99.4%) was obtained from Erimos Pharmaceuticals (Houston, TX). Sorafenib-methyl-d3 was purchased from Toronto Research Chemicals (Toronto, ON). HPLC grade acetonitrile, methanol, and formic acid were purchased from EMD Chemical Inc. (Billerica, MA). Deionized water was obtained from the Millipore Milli-Q-UF filtration system (Milford, MA). All other chemicals were of molecular biological grade or higher and were obtained from Sigma–Aldrich (St. Louis, MO). Drug-free human serum, sodium heparin human plasma, and potassium EDTA human plasma were purchased from Biological Specialty Company (Colmar, PA, USA).

All analytes had a purity greater than 98%. Navtemadlin was purchased from MedChemExpress (Monmouth Junction, NJ), and the stable isotope D₆-Navtemadlin was obtained from Moravek (Brea, CA). Authentic Navtemadlin glucuronide reference standard was unavailable. Navtemadlin glucuronide was therefore generated from a human liver microsome experiment using standard methods [12 (link)]. HPLC grade acetonitrile and formic acid were purchased from EMD Chemical Inc. (Billerica, MA). Molecular biological grade dimethyl sulfoxide (DMSO) was purchased from Sigma Aldrich (St. Louis, MO). Deionized water was obtained from Millipore Milli-Q-UF filtration system (Milford, MA). Pooled human liver microsomes and cofactors for the incubations were purchased from Corning (Glendale, AZ). Drug-free sodium EDTA human plasma was purchased from Biological Specialty Company (Colmar, PA, USA).