Nicolet is50 ft ir analyzer

The NIR spectra were measured while using a Nicolet iS50 FT-IR analyzer (Thermo Fisher Scientific, Waltham, MA, USA) that was equipped with an indium gallium arsenide detector and an integrating sphere. The instrument was controlled by OMNIC 9.2 software (Thermo Fisher Scientific, Waltham, MA, USA).
A sample (glass slide up) was placed on the detection window of the integrating sphere and was covered by a lid with a gold inner top. The transflectance spectra for the samples were measured in the range of 12,000–4000 cm⁻¹ while using the selected resolution and the selected number of co-added scans. The resolution was selected from 2, 4, 8, and 16 cm⁻¹ to obtain sufficient sample information in a shorter time; the number of co-added scans was selected from 16, 32, 64, and 128 to reduce the noise in a shorter time. Each sample was measured at three tissue locations. The mutant and wild-type samples were alternatively measured. The background spectrum was measured, prior to the sample spectra, under the same conditions to eliminate any ambient interferences on the sample spectra.