Spectra x led lamp

Manufactured by Lumencor

Sourced in Germany

The Spectra X LED lamp is a high-performance illumination source designed for laboratory and research applications. It features a compact, modular design and provides a broad spectrum of illumination across the visible and near-infrared wavelengths. The Spectra X delivers high-intensity, uniform illumination with precise control over intensity and spectral output.

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Lab products found in correlation

Orca flash 4.0 cmos camera, by Hamamatsu Photonics (2 mentions) Eclipse ti, by Nikon (1 mentions) Stemi 2000 c stereomicroscope, by Zeiss (1 mentions) Coolsnap hq camera, by Teledyne (1 mentions) Axio imager m1 microscope, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Mitotracker orange cmtmros, by Thermo Fisher Scientific (1 mentions) Axiocam erc5s digital camera, by Zeiss (1 mentions) Metamorph, by Universal Imaging (1 mentions) Axio imager m1, by Zeiss (1 mentions) Photometrix cool snaphq camera, by Teledyne (1 mentions) Zen 2 core, by Zeiss (1 mentions) Axiocam erc5, by Zeiss (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) A1 confocal system, by Nikon (1 mentions)

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Spectra X (140 citations)

6 protocols using spectra x led lamp

Epifluorescence Microscopy for Quantitative Cell Imaging

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Imaging was done using a Nikon Eclipse Ti inverted widefield epifluorescence microscope with either a 60× oil immersion NA1.4 plan apochromat or 40× air NA 0.6 plan fluor objective. A Spectra X LED lamp (Lumencor) was used as the light source, attenuated with ND2 or ND4 filters as necessary. Images were captured by a Hamamatsu Orca-Flash 4.0 CMOS camera. NIS Elements Advanced Research version 4.30 was used for microscope controlling and image acquisition. Qualitative images were generated by obtaining a multichannel Z-stack and performing blind 3D non-iterative deconvolution using algorithms from AutoQuant (Media Cybernetics/Roper Industries, Inc.) within NIS Elements Advanced Research 4.30. Images were captured in a 16-bit non-compressed tagged-image format (TIFF/.tif) and converted to bitmaps (.bmp) or JPEGs (.jpg) using ImageJ64 (National Institutes of Health) prior to analysis. Multichannel images were split into their individual channels using the ImageJ64 (National Institutes of Health) channel splitting function. Images were analyzed using PhenoRipper by selecting three images per cell type at random for thresholding with a block size of 15.

Nath S., Caron N.S., May L., Gluscencova O.B., Kolesar J., Brady L., Kaufman B.A., Boulianne G.L., Rodriguez A.R., Tarnopolsky M.A, & Truant R. (2022). Functional characterization of variants of unknown significance in a spinocerebellar ataxia patient using an unsupervised machine learning pipeline. Human Genome Variation, 9, 10.

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Fluorescence Microscopy of Fungal Morphogenesis

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Fluorescence microscopy was performed using an AxioImager M.1 microscope (Zeiss) equipped with a CoolSnap HQ camera (Roper Scientific) and a SpectraX LED lamp (Lumencor). Images were captured and edited with MetaMorph (Universal Imaging). For localization of PRO34, strains were grown on BMM-covered slides [31 (link)] for two days. Mitochondria were stained with 100 µM MitoTracker orange CMTMRos (Life Technologies, Darmstadt, Germany). GFP and MitoTracker fluorescence was analyzed using Chroma filter sets (Chroma Technology Corp.) 41017 (HQ470/40, HQ525/50, Q495lp) and 49008 (HQ560/40, ET630/75m, T585lp), respectively.
Hyphal fusion was observed after two days of growth on MMS with cellophane as described using the AxioImager M.1 microscope (Zeiss) [27 (link)].
Perithecia formation was assayed on BMM plates after seven days of growth using a Stemi 2000-C stereomicroscope (Zeiss) equipped with an AxioCamERc5s digital camera (Zeiss) and AxioVision software (Zeiss). Ascospore formation was assayed after ten days of growth on BMM plates. Perithecia were cracked open and ascus rosettes were imaged on slides using the AxioImager M.1 microscope (Zeiss). Images were processed with Adobe CS4 and CS6 (Adobe Corp.).

Hamann A., Osiewacz H.D, & Teichert I. (2022). Sordaria macrospora Sterile Mutant pro34 Is Impaired in Respiratory Complex I Assembly. Journal of Fungi, 8(10), 1015.

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Quantifying Fungal Fruiting Body Development

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The initiation of fruiting body development was quantified after 3 days of incubation on BMM-covered microscope slides. Ascogonia and protoperithecia were counted separately within an area of 0.5 cm² located 1 cm behind the growth front. An Axio Imager M1 (Zeiss) with a SpectraX LED lamp (Lumencor) and a Photometrix Cool SnapHQ camera (Roper Scientific, Martinsried, Germany) was used for quantification. Experiments were repeated for three biological replicats per strain. For assessment of fruiting body formation, images of strains were taken after 7 days of incubation in Petri dishes on solid BMM with a Zeiss Stemi 2000-C binocular, using an AxioCam ERc5s with the software ZEN 2 core (version 2.5, Zeiss, Jena, Germany).

Schmidt S., Märker R., Ramšak B., Beier-Rosberger A.M., Teichert I, & Kück U. (2020). Crosstalk Between Pheromone Signaling and NADPH Oxidase Complexes Coordinates Fungal Developmental Processes. Frontiers in Microbiology, 11, 1722.

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Immunofluorescence Analysis of Fibroblasts

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Immunofluorescence was performed with Hoechst 33342 (ThermoFisher), beta-tubulin antibody (E7, DSHB, 1:250 dilution in 2% FBS in PBS with 0.02% Tween), and N17-phospho antibody (NEP, validated in Supplemental Figure 3). Images were analyzed using Phenoripper. Five images per trial of TruHD fibroblasts acquired on a Nikon TiEclipse inverted epifluorescence widefield using a 20× objective (NA = 0.75) and Spectra X LED lamp (Lumencor) capture using an Orca-Flash 4.0 complementary metal oxide semiconductor (CMOS) camera (Hamamatsu). Cell surface area was calculated with ImageJ. Cells were thresholded to remove background to identify the whole-cell region of interest, and the area of each cell was measured and plotted.

Hung C.L., Maiuri T., Bowie L.E., Gotesman R., Son S., Falcone M., Giordano J.V., Gillis T., Mattis V., Lau T., Kwan V., Wheeler V., Schertzer J., Singh K, & Truant R. (2018). A patient-derived cellular model for Huntington’s disease reveals phenotypes at clinically relevant CAG lengths. Molecular Biology of the Cell, 29(23), 2809-2820.

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Quantifying Nuclear Speckles in TruHD Cells

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TruHD cells were treated with 0.1 mM 3NP for 1 h at 37°C and then fixed and permeabilized with ice-cold methanol for 12 min. Cells were washed in PBS and blocked in antibody solution (2% FBS, 0.1% [vol/vol] Triton X-100 in 1X TBS) at room temperature for 10 min. AlexaFluor488-conjugated N17-phospho antibody was diluted 1:15 in antibody solution and incubated overnight at 4°C. Cells were washed in PBS and stained with Hoechst 33342 dye for 12 min at room temperature and left in PBS prior to imaging.
Cells were imaged using Nikon TiEclipse inverted widefield epifluorescence microscope using a Plan Apo 60X (NA = 1.4) oil objective and Spectra X LED lamp (Lumencor) captured on an Orca-Flash 4.0 CMOS camera (Hamamatsu). A z-stack was obtained for each image and displayed as a maximum projection prior to image analysis. Image acquisition was completed using the NIS-Elements Advanced Research 4.30.02v 64-bit acquisition software (Nikon). Nuclear speckles were quantified in over 200 cells over three trials using an open-source speckle counting pipeline in CellProfiler (www.cellprofiler.org).

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Confocal Microscopy Imaging and Irradiation

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All microscopy was completed using a Nikon A1 confocal system attached to a Nikon Eclipse Ti inverted microscope, using an PLAN APO 60x/1.40 oil objective or PLAN APO 20×/0.75 dry objective with Spectra X LED lamp (Lumencor) and GaAsP detectors. A 405nm laser which was part of the Nikon A1 confocal system was used for irradiation experiments. 405nm, 489nm, and 561nm lasers experiments were used for imaging.

Suart C.E., Perez A.M., Al-Ramahi I., Maiuri T., Botas J, & Truant R. (2021). Spinocerebellar Ataxia Type 1 protein Ataxin-1 is signaled to DNA damage by ataxia-telangiectasia mutated kinase. Human molecular genetics, 30(8).

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