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Human mmp 3 quantikine elisa kit

Manufactured by R&D Systems
Sourced in United States

The Human MMP-3 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure human matrix metalloproteinase-3 (MMP-3) levels in cell culture supernates, serum, and plasma. The kit utilizes a microplate pre-coated with a monoclonal antibody specific for human MMP-3.

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2 protocols using human mmp 3 quantikine elisa kit

1

Quantifying Inflammatory Cytokines and MMPs

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Protein levels of human IL-1β and IL-6 in cell-free FLS supernatants were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits (NeoBioscience, Beijing, China) according to the manufacturer's instructions. MMP-1 was quantified by fluorescence assay using the Human Active MMP-1 Fluorokine E kit according to the manufacturer's protocol (R&D Systems, Minneapolis, MN, USA). MMP-3 expression was determined using a Human MMP-3 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA).
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2

Quantifying MMP-1 and MMP-3 Levels

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Cells were pretreated with the EFE for 1 h and then treated with IL-1β at 37°C for 24 h. Cell culture medium was collected after removing the particulates. MMP-1 and MMP-3 levels in the culture supernatants were determined using a Human Active MMP-1 Fluorokine E Kit and Human MMP-3 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocols.
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