Ab5074 p

Manufactured by Merck Group

Sourced in United States

AB5074 P is a versatile laboratory equipment designed for various scientific applications. It functions as a precision instrument for accurate measurement and analysis. The core functionality of this product is to provide reliable and consistent performance in laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

Mn1020, by Thermo Fisher Scientific (2 mentions) Mp 7402, by Vector Laboratories (1 mentions) 3 amino 9 ethylcarbazol hrp substrate kit, by Vector Laboratories (1 mentions)

3 protocols using ab5074 p

Immunohistochemical Analysis of Temporal Lobe Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the imaging was completed the scanned temporal lobes were sectioned coronally in 5 mm slabs using a motor-driven rotary slicer beginning at the level of pes hippocampi as previously described [37 (link)]. The slabs were embedded in paraffin and 6-μm sections cut from them using a microtome. The slices were mounted on Superfrost Plus glass slides. Four consecutive slides from each slab were stained with hematoxylin and eosin, cresyl violet, amyloid β 1-40 antibody (AB5074 P, EMD Millipore Corporation, Billerica, USA), and tau antibody (MN1020, Thermo Fisher Scientific Pierce, Rockford, IL, USA). Appropriate horseradish peroxidase-linked secondary antibodies (Vector MP7401 and MP7402, Vector Laboratories, Burlingame, CA, USA) and 3,3′-diaminobenzidine Peroxidase Substrate Kit (Vector SK-4100, Vector Laboratories, Burlingame, CA, USA) was utilized to visualize immunostaining.

Apostolova L.G., Zarow C., Biado K., Hurtz S., Boccardi M., Somme J., Honarpisheh H., Blanken A.E., Brook J., Tung S., Lo D., Ng D., Alger J.R., Vinters H.V., Bocchetta M., Duvernoy H., Jack CR J.r., Frisoni G., Bartzokis G., Csernansky J.G., de Leon M.J., deToledo-Morrell L., Killiany R.J., Lehéricy S., Malykhin N., Pantel J., Pruessner J.C., Soininen H, & Watson C. (2015). Relationship between hippocampal atrophy and neuropathology markers: A 7T MRI validation study of the EADC-ADNI Harmonized Hippocampal Segmentation Protocol. Alzheimer's & dementia : the journal of the Alzheimer's Association, 11(2), 139-150.

+ Open protocol

+ Expand

Pathological Diagnosis of Alzheimer's Disease

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pathologic diagnosis of AD was determined based on Braak and Braak (Braak and Braak, 1991 (link)) and Consortium to Establish and Registry for AD (CERAD) pathologic criteria (1991). The temporal lobes were sectioned coronally into 5 mm slabs, embedded in paraffin from which 6 sections were cut (Zarow et al., 2005 (link)). The tissue was stained with cresyl violet, hematoxylin and eosin, Aβ1–40 antibody (AB5074 P, EMD Millipore Corporation, Billerica, USA), and tau antibody (MN1020, Thermo Fisher Scientific Pierce, Rockford, IL, USA). To then visualize the immunostaining, horseradish peroxidase-linked secondary antibodies (Vector MP7401 and MP7402, Vector Laboratories, Burlingame, CA, USA) and 3,3′-diaminobenzidine Peroxidase Substrate Kit (Vector SK-4100, Vector Laboratories, Burlingame, CA, USA) were used as previously described.
Hippocampal subfields (CA1, CA2, CA3, CA4, and subicular pyramidal cell layer) were manually outlined on each slice using the Aperio ImageScope® CS as previously described (Apostolova et al., 2015 (link)). Amyloid and tau burden in each subfield were determined with Aperio Positive Pixel Count Algorithm. Neuronal counts were determined using the Aperio IHC Nuclear Image Analysis algorithm followed by visual inspection and manual correction of mislabeled nuclei.

Blanken A.E., Hurtz S., Zarow C., Biado K., Honarpisheh H., Somme J., Brook J., Tung S., Kraft E., Lo D., Ng D.W., Vinters H.V, & Apostolova L.G. (2017). Associations between hippocampal morphometry and neuropathologic markers of Alzheimer's disease using 7 T MRI. NeuroImage : Clinical, 15, 56-61.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Neurodegenerative Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human tissue was fixed in periodate-lysine-paraformaldehyde, and tissue blocks were paraffin-embedded and cut at 10 µm for immunohistochemistry. Antigen retrievals were performed by formic acid treatment for 2 min for Aβ antibodies. Sections were incubated at 4 °C overnight with antibodies to phosphorylated PHF-tau (AT8; Pierce Endogen, Rockford IL; 1:2000), Aβ1–40 (AB5074P; EMD Millipore, Billerica, MA; 1:2000), Aβ1–42 (AB5078P; EMD Milli-pore; 1:2000), alpha-synuclein (rabbit polyclonal; Chemi-con, Temecula, CA; 1:15,000), and pTDP-43 (pS409/410 mouse monoclonal; Cosmo Bio Co, Tokyo, Japan; 1:2000). For determination of Aβ deposition in the medial temporal lobe (section of the hippocampal formation, parahippocampal gyrus, transentorhinal region, and portions of occipito-temporal gyrus), the monoclonal anti-Aβ antibody (Clone 4G8; Covance, Dedham, MA, 1:100,000) was used. After three washes with PBS (pH 7.4), sections were treated with biotinylated secondary antibody and labeled with a 3-amino-9-ethylcarbazol HRP substrate kit (Vector Laboratories). Sections were counterstained with Gill’s Hematoxlin (Vector Laboratories H-3401) and then coverslipped with Permount mounting medium.

Stein T.D., Montenigro P.H., Alvarez V.E., Xia W., Crary J.F., Tripodis Y., Daneshvar D.H., Mez J., Solomon T., Meng G., Kubilus C.A., Cormier K.A., Meng S., Babcock K., Kiernan P., Murphy L., Nowinski C.J., Martin B., Dixon D., Stern R.A., Cantu R.C., Kowall N.W, & McKee A.C. (2015). Beta-amyloid deposition in chronic traumatic encephalopathy. Acta neuropathologica, 130(1), 21-34.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!