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Anti cdk7

Manufactured by Cell Signaling Technology
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Anti-CDK7 is a monoclonal antibody that recognizes the cyclin-dependent kinase 7 (CDK7) protein. CDK7 is a subunit of the transcription factor TFIIH and plays a role in the regulation of transcription and cell cycle progression.

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Lab products found in correlation

Mouse monoclonal anti human β actin, by Merck Group (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti parp, by Santa Cruz Biotechnology (1 mentions) Anti ldhb, by Cell Signaling Technology (1 mentions) Anti bcl 2, by Cell Signaling Technology (1 mentions) Anti survivin, by Cell Signaling Technology (1 mentions) Anti xiap, by Cell Signaling Technology (1 mentions) Anti cdk2, by Cell Signaling Technology (1 mentions) Anti cdk4, by Cell Signaling Technology (1 mentions) Anti cyclin d2, by Cell Signaling Technology (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti pfkp, by Cell Signaling Technology (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Phosphatase inhibitor, by MedChemExpress (1 mentions) Proteinase inhibitor, by MedChemExpress (1 mentions) Anti α tubulin, by Cell Signaling Technology (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Anti cdk1, by Abcam (1 mentions)

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Anti-CDK7 antibody (2 citations)

3 protocols using anti cdk7

1

Comprehensive Protein Expression Analysis

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Western blot was performed as previously described (Lam et al., 2020 (link)). Specific primary antibodies [mouse monoclonal anti-human β-actin (Sigma-Aldrich); anti-PCNA, anti-PARP (Santa Cruz Biotechnology, Inc., Santa Cruz, California, United States of America); anti-PFKP, anti-LDHB, anti-Bcl-2, anti-survivin, anti-XIAP, anti-AKT, anti-CDK2, anti-CDK4, anti-CDK7, anti-Cyclin D2, H, anti-CDK4, anti-N-cadherin, anti-β-catenin (Cell Signaling Technology, Danvers, Massachusetts, United States of America); and corresponding horseradish peroxidase (HRP)-conjugated secondary (Cell Signaling Technology)] were purchased. An enhanced chemiluminescence (ECL) kit (GE Healthcare) was used to detect protein expression. Beta-actin was selected as a reference protein (Lam et al., 2020 (link)).
Lam S.K., Yan S., Lam J.S., Feng Y., Khan M., Chen C., Ko F.C, & Ho J.C. (2022). Disturbance of the Warburg effect by dichloroacetate and niclosamide suppresses the growth of different sub-types of malignant pleural mesothelioma in vitro and in vivo. Frontiers in Pharmacology, 13, 1020343.
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2

Western Blot Analysis of Cell Signaling Pathways

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Protein was extracted using RIPA buffer (Sangon Biotech, Shanghai, People’s Republic of China) containing proteinase inhibitors (MedChem Express) and phosphatase inhibitors (MedChem Express). The protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific). The following antibodies were used for Western blot: anti-CDK7 (Cell Signaling Technology, Danvers, MA, USA), anti-α-tubulin (Cell Signaling Technology), anti-poly(ADP-ribose) polymerase (anti-PARP) (Cell Signaling Technology), anti-cleaved PARP (Cell Signaling Technology), anti-RNA Pol II (Abcam, Cambridge, UK), anti-phospho RNA Pol II (Ser2) (Abcam), anti-phospho RNA Pol II (Ser5) (Abcam), anti-phospho RNA Pol II (Ser7) (Millipore), anti-CDK1 (Abcam), and anti-CDK1 (phospho T161) (Abcam).
Zhong S., Zhang Y., Yin X, & Di W. (2019). CDK7 inhibitor suppresses tumor progression through blocking the cell cycle at the G2/M phase and inhibiting transcriptional activity in cervical cancer. OncoTargets and therapy, 12, 2137-2147.
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3

Immunoblotting Protein Detection Protocol

Cited 1 time
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Immunoblotting to detect protein detection was performed using SDS-PAGE as previously described (Lal et al., 2014 (link)). Membranes for immunoblotting were probed with antibodies against anti-phospho-CTD-RNAPII-S2 (04-1571; Millipore), anti-phospho-CTD-RNAPII-S5 (04-1572; Millipore), anti-CDK7 (2916; Cell Signaling Technology), anti-CDK13 (A301-458A; Bethyl), anti-cleaved poly(ADP ribose) polymerase (Asp214; 9541; Cell Signaling Technology), anti-cleaved caspase 3 (Asp175; 9664; Cell Signaling Technology), NRF2 (12721; Cell Signaling Technology), and anti-α-tubulin (Sigma-Aldrich). Chemiluminescent (32106; Thermo Fisher Scientific) signal was captured using a Syngene G-BOX iChemi XT imager.
Zarei M., Du H., Nassar A.H., Yan R.E., Giannikou K., Johnson S.H., Lam H.C., Henske E.P., Wang Y., Zhang T., Asara J, & Kwiatkowski D.J. (2019). Tumors with TSC mutations are sensitive to CDK7 inhibition through NRF2 and glutathione depletion. The Journal of Experimental Medicine, 216(11), 2635-2652.
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