EGF was purchased from Peprotech (Rocky Hill, NJ, USA). LPS was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Fetal bovine serum (FBS), Trypsin/EDTA and antibiotics (Penicillin-Streptomycin for Cell Culture) were from GIBCO (Carlsbad, CA, USA). Dulbecco’s modified Eagle’s F12 Ham medium (HyCloneTM DMEM/F12 1:1 media) was purchased from GE Healthcare life sciences (South Logan, UT, USA). Plastic culture plates were manufactured by Corning Inc. (Corning, NY, USA). CCK-8 Assay Kit, BCA protein assay reagent, LDH Assay Kit, T-AOC Assay Kit, CAT Assay Kit, GSH-Px Assay Kit, SOD Assay Kit, and MDA Assay Kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). PBS, RIPA Lysis Buffer R2220 were purchased from Solarbio (Beijing, China). TRIzol Reagent was obtained from Invitrogen (Carlsbad, CA, USA). Annexin V-FITC/PI kits was obtained from Keygen Biotech (Nanjing, China).The primary antibodies against Nrf2, HO-1, NQO1, P53, Bax, Bcl2, Caspase3, β-actin, and the secondary antibody Goat Anti-Rabbit IgG/HRP used in Western blot analyses were all purchased from Proteintech (Rosemont, IL, USA). The primary antibody against Fas was purchased from Abcam (Cambridge, MA, USA).
Cck 8 assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The CCK-8 assay kit is a cell counting kit that measures cell viability and proliferation. It utilizes a water-soluble tetrazolium salt that produces a colorimetric change in the presence of viable cells. The kit provides a simple and sensitive method for quantifying the number of living cells in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Victor 1420, by PerkinElmer (3 mentions)
Microplate reader, by PerkinElmer (2 mentions)
Plastic culture plates, by Corning (1 mentions)
Hrp goat anti rabbit igg, by Proteintech (1 mentions)
Bca protein assay reagent, by Nanjing Jiancheng (1 mentions)
T aoc assay kit, by Nanjing Jiancheng (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Mda assay kit, by Nanjing Jiancheng (1 mentions)
Sod assay kit, by Nanjing Jiancheng (1 mentions)
Gsh px assay kit, by Nanjing Jiancheng (1 mentions)
Cat assay kit, by Nanjing Jiancheng (1 mentions)
Ldh assay kit, by Nanjing Jiancheng (1 mentions)
Sb216763, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Sw620, by American Type Culture Collection (1 mentions)
Fhc cells, by American Type Culture Collection (1 mentions)
15 protocols using cck 8 assay kit
1
Oxidative Stress and Apoptosis Assays
2
Colorectal Cancer Cell Lines and FHC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human CRC cell lines (SW480, SW620, HT29, LoVo and HCT116) and human normal colorectal mucosal FHC cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). SB216763 was purchased from Sigma-Aldrich (280744-09-4; HPLC >98%; Merck KGaA, Darmstadt, Germany). CCK-8 assay kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Transfection reagent Lipofectamine® 3000 was purchased from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA).
3
Cell Viability and LDH Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was determined using a CCK-8 Assay Kit (Jiancheng, Nanjing, China). Briefly, H9c2 cells were cultured and treated in 96-well plates, after which 10 µL of the CCK-8 reagent was added to each well, and the mixture was incubated for about 1 h in darkness to detect cell viability.
LDH in cultured media was measured using a colorimetric assay kit (Jiancheng, Nanjing, China) according to the manufacturer's instructions. Absorbance was spotted with the Perkin Elmer Microplate reader (EnSight PerkinElmer Victor 1420, USA).
LDH in cultured media was measured using a colorimetric assay kit (Jiancheng, Nanjing, China) according to the manufacturer's instructions. Absorbance was spotted with the Perkin Elmer Microplate reader (EnSight PerkinElmer Victor 1420, USA).
4
Lymphocyte Proliferation Assay with Tissue-Engineered Biomaterials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The design of MLR assay was according to Thaweesapphithak et al.17 (link) BALB/c spleen lymphocytes (SLCs) were treated with mitomycin C (50 µg/mL, Sigma) for 30 min at 37°C as stimulator cells. C57BL/6 SLCs were incubated with 0.5 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE, Sigma, St. Louis, MO) at 37°C for 15 min as responder cells. Stimulator and responder cells were co-cultured at a ratio of 1:1 in 96-well plates (5×105 cells/well). The mixed lymphocytes were incubated with RPMI 1640 medium and distributed to the following 6 groups: (1) vehicle control group (mixed DBMs with SLCs), (2) PHA (phytohemagglutinin, mixed SLCs incubated with 10 µg/mL PHA), (3) MSC-based TEBs (mixed SLCs with MSC-based TEBs), (4) MSC/POC-based TEBs (mixed SLCs with MSC/POC-based TEBs), (5) MSC ECM-based TEBs (mixed SLCs with MSC ECM-based TEBs), and (6) MSC/POC ECM-based TEBs (mixed SLCs with MSC/POC ECM-based TEBs). The cells were incubated with RPMI 1640 medium for five days, and cell viability was determined using a cell counting kit–8 (CCK–8) assay kit (Jiancheng Biotechnology, Nanjing, China).
5
Cell Viability and Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was assessed using a commercial CCK-8 assay kit (Nanjing Jiancheng Bioengineering Institute). The absorbance at wavelength of 450 nm was measured using a microplate reader. In addition, EdU assay was carried out to evaluate the proliferation ability of EC cells using the EdU Cell Proliferation Kit (YuhengBio). EdU-positive cells were counted and images were captured under a fluorescent microscope (Leica Microsystems GmbH).
6
HT22 Cell Viability Assay by CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HT22 cell viability was tested using the cell counting kit-8 (CCK-8) assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). HT22 cells were cultured in a 96-well plate (5 × 103 cells/well) for 24 h and then cultured in MR (0−100%) or HG (225 mM) culture medium for 12 h. Then, CCK-8 solution (10 μL/well) was added and incubated for 1.5 h in the CO2 incubator. Absorbance was measured using a microplate reader at 450 nm (Epoch, Biotek, Winooski, VT, USA). Cell viability (%) = [A(treatment) − A(blank)]/[A(control) − A(blank)].
7
Cell Viability Assay using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was determined using a CCK-8 Assay Kit (Jiancheng, Nanjing, China) in 96-well plates. After H9c2 cells were cultured and treated in 96-well plates, 10 μL of CCK-8 reagent was added to each well and then incubated for 3 h in darkness. The absorbance was detected at 450 nm using a Perkin Elmer Microplate reader (PerkinElmer Victor 1420, USA). The mean optical density (OD) of each group was used to calculate the percent of cell viability with the following formula: cell viability = treatment group OD/control group OD × 100%.
8
Cell Viability Assessment via CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells from different experimental subgroups were collected and according to the manufacturer's instructions, a CCK-8 assay kit (Jiancheng, Nanjing, China) was used to determine the cell viability in 96-well plates (the experimental procedure is as above).
9
Cell Viability Assay and Resistance Index
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was measured using a CCK-8 assay kit (Nanjing Jiancheng Bioengineering Research Institute Co., Ltd.). Cells were seeded in 96-well plates at a density of 1x105 cells/well and incubated at 37˚C for 24 h. Subsequently, 10 µl CCK-8 reagent was added to each well and incubated for 3 h at 37˚C. The absorbance of each was at 450 nm was measured using a microplate reader (PerkinElmer, Inc.) and the IC50 value was calculated using GraphPad Prism 8 software (GraphPad Software, Inc.). Finally, the resistant index (RI) was calculated according to the following formula: RI=IC50(SW1990/GZ)/IC50(SW1990).
10
Cell Viability Measurement via CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCK-8 assay kit (Jiancheng, Nanjing, China) was used to measure cell viability. After stimulation, the cultured cells in 96-well plates were given 10 μl CCK-8 reagent for each well and then incubated for 3 h in darkness. Perkin Elmer Microplate reader (PerkinElmer Victor 1420, USA) was used to analyse the absorbance at 450 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!