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Creatinine colorimetric detection kit

Manufactured by Enzo Life Sciences
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The Creatinine Colorimetric Detection Kit is a laboratory reagent used to quantitatively determine the concentration of creatinine in various biological samples, such as urine, serum, or plasma. The kit utilizes a colorimetric method to measure the absorbance of the reaction product, which is proportional to the creatinine concentration in the sample.

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Lab products found in correlation

Luminex based assay kit, by Merck Group (1 mentions) Epoch microplate spectrophotometer, by Agilent Technologies (1 mentions) Dri chem 4000i analyzer, by Fujifilm (1 mentions) Albuwell, by Exocell (1 mentions)

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Creatinine Detection Kit Colorimetric detection kit Creatinine

7 protocols using creatinine colorimetric detection kit

1

Creatinine Concentration in Urine Samples

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The urinary creatinine concentration was determined using the creatinine colorimetric detection kit (Enzo Life Sciences; ADI-907-030A, Farmingdale, New York, USA), according to the manufacturers’ instructions. Samples were diluted 1:20 with distilled/deionized water, and analyzed in duplicate. Four-parameter logistic standard curves were generated, and the creatinine concentrations within each urine sample were interpolated using GraphPad Prism version 5.00 (GraphPad Software, USA, www.graphpad.com). Healthy donors had a mean creatinine concentration of 186 mg/dL.
Ashiru O., Esteso G., García‐Cuesta E.M., Castellano E., Samba C., Escudero-López E., López‐Cobo S., Álvarez-Maestro M., Linares A., Ho M.M., Leibar A., Martínez‐Piñeiro L, & Valés‐Gómez M. (2019). BCG Therapy of Bladder Cancer Stimulates a Prolonged Release of the Chemoattractant CXCL10 (IP10) in Patient Urine. Cancers, 11(7), 940.
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2

Comprehensive Urinary Biomarker Assessment

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Urine NAG was measured using the NAG assay kit (Bio-quant, CA, USA). Urine creatinine was determined using a creatinine colorimetric detection kit (Enzo Life Sciences, NY, USA). Fibrinogen was measured using a Luminex-based assay kit (Millipore, MA, USA). Urinary protein biomarkers of Kim-1, NGAL, albumin, osteopontin, α-GST, GST-Yb1, RPA-1 and clusterin were measured using the rat multiplex assay kits and SECTOR™ Imager 2400 electrochemiluminescence detection platform (Meso Scale Discovery, MD, USA).
Zhang Q., Davis K.J., Hoffmann D., Vaidya V.S., Brown R.P, & Goering P.L. (2014). Urinary biomarkers track the progression of nephropathy in hypertensive and obese rats. Biomarkers in medicine, 8(1), 85-94.
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3

Urinary Creatinine Quantification Protocol

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To assess urinary creatinine concentrations, samples were diluted 20-fold and measured in duplicate using a creatinine colorimetric detection kit (Enzo Life Sciences) (using a modified Jaffe reaction). All creatinine readings were above the manufacturer’s limit of detection of 0.042 mg/dL (to convert to micromoles per liter, multiply by 88.4). The intra-assay precision was 1.0%, and the interassay precision was 3.4%. To adjust for urinary dilution, all arsenic measures were normalized and reported as micrograms per gram of creatinine.
Gump B.B., Heffernan K., Brann L.S., Hill D.T., Labrie-Cleary C., Jandev V., MacKenzie J.A., Atallah-Yunes N.H., Parsons P.J., Palmer C.D., Roberts A.A, & Bendinskas K. (2023). Exposure to Arsenic and Subclinical Cardiovascular Disease in 9- to 11-Year-Old Children, Syracuse, New York. JAMA Network Open, 6(6), e2321379.
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4

Quantification of Urinary PGE-M by LC/MS/MS

Cited 1 time
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Excreted metabolite of PGE2 in urine has been generally accepted as an index of endogenous PGE2 (3 (link), 16 (link)). A major urinary metabolite of PGE2 (11-α-hydroxy-9,15-dioxo-2,3,4,5-tetranor-prostane-1,20-dioic acid; PGE-M)(17 (link)) was quantified using liquid chromatography/tandem mass spectrometry (LC/MS/MS) in the Eicosanoid Laboratory at Vanderbilt University School of Medicine using methods as previously described (3 (link), 18 (link)).
A set of twenty samples was analyzed in each run and approximately 20% (N=127) of the total samples were randomly selected and analyzed in duplicate. The coefficient of variation (%CV) of PGE-M among duplicates was 6% within-run and 14% between-runs. Urinary creatinine was measured in duplicate using the Creatinine Colorimetric Detection Kit supplied by Enzo Life Sciences (P/N ADI1907030A) to adjust urinary PGE-M concentration for creatinine concentration (ng PGE-M/mg creatinine).
Kim S., Rimando J, & Sandler D.P. (2015). Fruit and vegetable intake and urinary levels of prostaglandin E2 metabolite in postmenopausal women. Nutrition and cancer, 67(4), 580-586.
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5

Mouse Serum and Urine Creatinine Analysis

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After collecting blood from each mouse, the blood was allowed to stand at room temperature for 30 min, and then centrifuged at 6,000 rpm at 4 °C to collect upper serum. Serum creatinine and BUN levels were analyzed by FUJI DRI-CHEM 4000i analyzer (FUJIFILM Corp., Tokyo, Japan). On the other hand, urine creatinine level was detected by Creatinine Colorimetric Detection kit (Enzo Biochem, NY) according to the manufacturer protocol. Briefly, the mouse urine was diluted with deionized water to a final 50 µl reaction volume in a 96-well plate. For each well, 100 µl of Creatinine Detection Reagent was added and incubated for 30 min. Plate-based colorimetric measurement (490 nm) was performed by Epoch microplate spectrophotometer (Biotek, VT).
Chiou Y.Y., Jiang S.T., Ding Y.S, & Cheng Y.H. (2020). Kidney-based in vivo model for drug-induced nephrotoxicity testing. Scientific Reports, 10, 13640.
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6

Urinary Albumin and Creatinine Measurement

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The urinary albumin and creatinine were measured using the Albuwell (Exocell Inc., PA, USA) and by creatinine colorimetric detection kit (Enzo Life Science, NY, USA), respectively. The urine samples were collected from the mice housed in individual metabolic cages. Urinary albumin and creatinine were measured at 11 weeks old (before treatment of EPA-E) and 28 weeks old (after 15 weeks of EPA-E treatment).
Yasuzawa T., Nakamura T., Ueshima S, & Mima A. (2021). Protective Effects of Eicosapentaenoic Acid on the Glomerular Endothelium via Inhibition of EndMT in Diabetes. Journal of Diabetes Research, 2021, 2182225.
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7

Creatinine Assay Validation in Clinical Samples

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For creatinine, 610 urine samples from 53 healthy individuals19 (link),20 (link), 189 patients with diabetes mellitus [Petrucci et al. submitted], 110 patients with hematologic21 (link),22 (link), and 258 with solid cancers10 (link), were assayed for a second time for creatinine using a commercial kit (Creatinine Colorimetric Detection Kit; Enzo Life Sciences, Farmingdale, NY, USA). The inter-assay coefficient of variation using a commercial standard (Creatinine Standard, Cayman Chemical) was 7% over the study duration (n = 523 determinations).
Petrucci G., Hatem D., Langley R., Cleary S., Gentry-Maharaj A., Pitocco D., Rizzi A., Ranalli P., Zaccardi F., Habib A, & Rocca B. (2024). Effect of very long-term storage and multiple freeze and thaw cycles on 11-dehydro-thromboxane-B2 and 8-iso-prostaglandin F2α, levels in human urine samples by validated enzyme immunoassays. Scientific Reports, 14, 5546.
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