Anti smad3

Manufactured by Thermo Fisher Scientific

Anti-Smad3 is a laboratory reagent used for the detection and analysis of the Smad3 protein, which is a key mediator of the transforming growth factor-beta (TGF-β) signaling pathway. It is commonly used in cell biology and biochemical research applications.

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Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions) Bradford assay, by Thermo Fisher Scientific (1 mentions) Gapdh, by Abcam (1 mentions) Nitrocellulose membrane filter par sandwich, by Thermo Fisher Scientific (1 mentions) Anti smad4, by Abcam (1 mentions) Anti smad7, by Merck Group (1 mentions) Anti tgf β ri, by Abcam (1 mentions) Anti tgf β rii, by Abcam (1 mentions) Anti tgf β2, by Abcam (1 mentions) Anti tgf β3, by Abcam (1 mentions) Anti tgf β1, by Abcam (1 mentions) Anti actin mab, by Merck Group (1 mentions) Anti ha mab, by Fortrea (1 mentions) Anti smad4, by Santa Cruz Biotechnology (1 mentions) Anti smad2, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Smad3 (17 citations)

2 protocols using anti smad3

Western Blot Analysis of TGF-β Signaling Pathway

Cited 2 times

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Western blot analyses of HCF and HKC cells were performed with lysis of cells, as previously described [27 (link),31 (link),32 ]. Protein concentration and purity were assessed by Bradford assay (Thermo Scientific, IL). As well, 4%–20% Tris-Glycine gels (Novex, Life technologies, Carlsbad, CA) was used for gel electrophoresis, to which equal amounts of proteins were loaded and a protein transfer was done using Nitrocellulose membrane (Novex, Nitrocellulose membrane filter par sandwich, Life Technologies). After incubation in a 5% BSA blocking solution (Thermo Scientific), the membranes were incubated with primary rabbit antibodies (Table 2): anti-Smad3 (Invitrogen, Camarillo, CA), anti-SMAD7 (Sigma-Aldrich, Saint Louis, MO), anti-SMAD4 (Abcam, Cambridge, MA), anti-SMAD6 (Abcam), Anti-TGF-βRI (Abcam), Anti-TGF-βRII (Abcam), Anti-TGF-βRIII (Abcam), anti-TGF-β1 (Abcam), anti-TGF-β2, and anti-TGF β3 (Abcam) at 1:1,000 dilution overnight at 4 °C separately. This was followed by washing of the membranes and incubation with a secondary antibody (Alexa Flour® 568 Donkey anti-Rabbit, IgG [H⁺L], Abcam) at 1:2,000 dilutions for 1 h. The Kodak imaging system was used for detecting the antibody binding to the membrane. GAPDH (Abcam) was used as the loading control and results were analyzed by normalizing the value to that of the loading control expression and plotting the fold expression.

Priyadarsini S., McKay T.B., Sarker-Nag A, & Karamichos D. (2015). Keratoconus in vitro and the key players of the TGF-β pathway. Molecular Vision, 21, 577-588.

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Comprehensive Antibody Immunostaining Protocol

Cited 1 time

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Antibodies are rabbit polyclonal or mouse monoclonal (mAb), unless specified. From Covance: mAb anti-HA conjugated to fluorescein-isothiocyanate (cat# FITC-101L; 1:50 dilution) and mAb anti-HA (#MMS-101R; 1:500 IB and 1:100 IF); from BD Biosciences: mAb anti-Orc4 (#611171; 1:500); from BD Transductions: mAb anti-Cdk2 (#610146; 1:500); from Cell Signaling: mAb anti-Histone H4 (#2935; 1:500), anti-Mcm3 (#4012; 1:200), anti-Mcm4 (#12973; 1:500), anti-Smad2 (#5339; 1:1000), and rabbit monoclonal anti-Smad3 (#9523; 1:200); from Invitrogen: anti-BrdU-Alexa488 (#B35130; 1:20) and anti-BrdU-Alexa594 (#B35132; 1:20); from Calbiochem: anti-Rb (#PC13 ; 1:500); from Millipore: rabbit monoclonal anti-Smad4 (#04–1033; 1:750); from Santa Cruz Biotechnology: mAb anti-Mcm6 (#sc-55577; 1:200), mAb anti-Mcm7 (#sc-9966; 1:1000), anti-Cdc6 (#sc-8341; 1:300), anti-Cdc6-serine54P (#sc-12920R; 1:500), anti-CycE (agarose-conjugated; #sc-481; 1:20), mAb anti-Cyclin E (#sc-377100; 1:100),and anti-Cdt1 (#sc-28262; 1:300); from Sigma: mAb anti-Actin (#A5316; 1:5000); anti-Mcm2 (1:1000) was generated by our group and validated previously (27 (link)); anti-Mcm5 (1:2000) was provided by Rolf Knippers (University of Konstanz, Germany); anti-Myc (1:3000) was provided by Steve Hann (Vanderbilt University); anti-HBO1 (1:200) was provided by Masayoshi Iizuka (Teikyo University School of Medicine).

Nepon-Sixt B.S, & Alexandrow M.G. (2018). TGFß1 Cell Cycle Arrest Is Mediated by Inhibition of MCM Assembly in Rb-deficient Conditions. Molecular cancer research : MCR, 17(1), 277-288.

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