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Hplc grade

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HPLC grade is a type of laboratory-grade solvent or chemical used in High-Performance Liquid Chromatography (HPLC) applications. These solvents are specifically formulated to meet the high purity and low impurity requirements necessary for HPLC analysis.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (26 mentions) Methanol, by Thermo Fisher Scientific (26 mentions) Formic acid, by Thermo Fisher Scientific (23 mentions) Formic acid, by Merck Group (6 mentions) Hplc grade, by Merck Group (6 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (5 mentions) Milli q water purification system, by Merck Group (4 mentions) Milli q water, by Merck Group (4 mentions) Optima lc ms grade, by Thermo Fisher Scientific (4 mentions) Prominence series hplc system, by Shimadzu (4 mentions) Lc 20ab binary pump, by Shimadzu (4 mentions) Sil 20a ht autosampler, by Shimadzu (4 mentions) Acetic acid, by Thermo Fisher Scientific (4 mentions) Gallic acid, by Merck Group (3 mentions) Folin ciocalteu reagent, by Merck Group (3 mentions) Milli q system, by Merck Group (3 mentions) Sodium hydroxide (naoh), by Tianjin Zhiyuan (3 mentions) Piperaquine tetraphosphate tetrahydrate, by AK Scientific (3 mentions) Cto 20ac temperature controlled column oven, by Shimadzu (3 mentions) Cbm 20a communications bus, by Shimadzu (3 mentions)

Close spelling variants of this product

Optima HPLC grade acetonitrile HPLC gradient grade Methanol and acetonitrile (HPLC grade) Tetrahydrofuran (HPLC grade) HPLC grade water and acetonitrile HPLC-MS-grade acetonitrile HPLC grade ACN Phosphoric acid (HPLC grade) HPLC-grade acetonitrile (CH3CN) Methanol and acetic acid (HPLC grade)

157 protocols using hplc grade

1

Preparation of 2-Nonanone and Alkane Standard Solutions

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First, 0.1 g of 2-nonanone (accurate to 0.0001 g) was diluted with 10 mL of methanol (HPLC grade, Thermo Fisher Scientific, Waltham, MA, USA) to prepare a 10,000 mg/L stock solution. From this, a 10 mg/L working solution of 2-nonanone was prepared using methanol (HPLC grade, Thermo Fisher Scientific, Waltham, MA, USA). Then, 500 μg/mL of C8–C40 n-alkane mix solutions and 2.0 mg/mL of C6–C10 n-alkanes were diluted to 10 mg/L with acetone (Analytical reagent, Sinopharm, China). All standard solutions were stored at −18 °C.
Zhang W., Yan M., Zheng X., Chen Z., Li H., Mao J., Qin H., Zhu C., Du H, & Abd El-Aty A.M. (2023). Exploring the Aroma Fingerprint of Various Chinese Pear Cultivars through Qualitative and Quantitative Analysis of Volatile Compounds Using HS-SPME and GC×GC-TOFMS. Molecules, 28(12), 4794.
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2

Solvent Purification and Preparation

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Reaction solvents tetrahydrofuran
(Fisher, HPLC grade) and CH2Cl2 (Fisher, unstabilized
HPLC grade) were dried by passage through two columns of neutral alumina
in a solvent-dispensing system. Reaction solvent deuterated chloroform
(CDCl3, Cambridge Isotope Laboratories, D 99.8%) was dried
by keeping it with activated 4 A MS at least over 24 h. Solvents for
chromatography, filtration, and recrystallization were CH2Cl2 (Aldrich, ACS grade), ethyl acetate (Fisher, ACS grade),
pentane (Fisher, HPLC grade), and hexanes (Fisher, Optima) and used
as received.
Denmark S.E, & Chi H.M. (2017). Catalytic, Enantioselective, Intramolecular Sulfenoamination of Alkenes with Anilines. The Journal of Organic Chemistry, 82(7), 3826-3843.
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3

Extraction of Secondary Metabolites from Pseudomonas

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Frozen stocks of Pseudomonas spp. were inoculated into 600 µL of liquid Tryptic Soy Broth (TSB, Bacto Soybean-Casein Digest Medium, 30 g / liter) in 2.0 mL 96 deep well plates (Thermo Scientific, Nunc 2.0 mL DeepWell Plate). Cultures were grown overnight at 30°C and 200 rpm and then diluted 500x into a second 2.0 mL 96 deep well plate containing fresh TSB liquid. 5 µL of the 500x dilution was inoculated into a third 2.0 mL 96 deep well plate containing 600 µL TSB agar (15 g agar / liter), sealed with 96 Well-Cap Mats (Thermo Scientific, Nunc 96 Well-Cap Mats), and incubated at 30°C for 72 hours. The cultures were extracted with 300 µL 50/50 v/v ethyl acetate (Fisher Scientific, HPLC grade)/methanol (Fisher Scientific, HPLC grade). The plates were resealed with the same 96 Well-Cap Mats, sonicated for 10 minutes, and extracted for an additional 50 minutes. 250 µL of these crude extracts were transferred into a pre-washed 96 well plate (Agilent Technologies, 96 well plates, 0.5 mL, polypropylene) and lyophilized to dryness. The extract protocol was repeated once more for a total extract volume of 500 µL.
Nguyen D.D., Melnik A.V., Koyama N., Lu X., Schorn M., Fang J., Aguinaldo K., Lincecum TL J.r., Ghequire M.G., Carrion V.J., Cheng T.L., Duggan B.M., Malone J.G., Mauchline T.H., Sanchez L.M., Kilpatrick A.M., Raaijmakers J.M., De Mot R., Moore B.S., Medema M.H, & Dorrestein P.C. (2016). Indexing the Pseudomonas specialized metabolome enabled the discovery of poaeamide B and the bananamides. Nature microbiology, 2, 16197.
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4

Solvent Purification for Organic Synthesis

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Reaction solvents tetrahydrofuran (Fisher, HPLC grade, BHT stabilized), diethyl ether (Fisher, ACS grade, BHT stabilized), and dichloromethane (Fisher, HPLC grade, not stabilized) were dried by percolation through two columns packed with neutral alumina under a positive pressure of argon. N,N-Dimethylformamide (Fisher, ACS grade) was dried by percolation through two columns packed with molecular sieves. Methanol and ethanol were distilled from magnesium turnings under a nitrogen atmosphere. Pyridine and acetonitrile were distilled from calcium hydride under a nitrogen atmosphere. Solvents for filtration, transfers, chromatography, and recrystallizations were purchased from commercial sources and used as received. “Brine” refers to a saturated solution of sodium chloride in distilled water.
Hilby K.M, & Denmark S.E. (2021). Lewis Base Catalyzed, Sulfenium Ion Initiated Enantioselective, Spiroketalization Cascade. The Journal of organic chemistry, 86(21), 14250-14289.
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5

HPLC Characterization of Dynantin and MDP

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All analyses were performed using a Shimadzu Prominence series HPLC system (Shimadzu Corporation, Kyoto, Japan), equipped with a LC-20AB binary pump (Serial: L20124200883), SIL-20A HT autosampler (Serial: L20345256104), CTO-20AC temperature controlled column oven (Serial: L2021525077), and CBM-20A communications bus (Serial: L20235154327). All equipment was controlled by Shimadzu LabSolutions Lite software version 5.71 SP2. For separation, an Ultra C18 column, 3 μm, 50 x 4.6 mm (RESTEK Corporation, Bellefonte, PA) was used.
Dynantin samples were analyzed at a constant solvent flow rate of 0.7 mL/min at 35 °C using a binary gradient (Table 1). Solvent A consisted of a 25% solution of acetonitrile (HPLC grade Fisher Scientific) in ddH2O (0.2 μm filtered) and solvent B consisted of acetonitrile, with each solvent containing 0.1% trifluoroacetic acid (v/v, protein sequencing grade, Sigma Aldrich, Fairlawn NJ).
MDP samples were analyzed at a constant solvent flow rate of 1.0 mL/min at 35 °C using a binary gradient (Table 2). Solvent A consisted of ddH2O (0.2 μm filtered) and solvent B consisted of methanol (MeOH) (HPLC grade, Fisher Scientific, Fairlawn NJ), with each solvent containing 0.1% formic acid (v/v, LC/MS grade, Fisher Scientific).
Lewicky J.D., Martel A.L., Fraleigh N.L., Boraman A., Nguyen T.M., Schiller P.W., Shiao T.C., Roy R, & Le H.T. (2018). Strengthening peptide-based drug activity with novel glyconanoparticle. PLoS ONE, 13(9), e0204472.
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6

Catalytic H2O2 Degradation Assay

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Catalytic activity toward
H2O2 degradation was determined in a similar
way to the direct synthesis activity of a catalyst. The autoclave
was charged with the catalyst (0.01 g), MeOH (5.6 g, HPLC grade, Fisher
Scientific), H2O2 (50 wt %, 0.69 g, Merck),
and water (2.21 g, HPLC grade, Fisher Scientific). Prior to the addition
of the catalyst to the reaction solution, three aliquots (0.05 g)
were removed from the solution and titrated with acidified Ce(SO4) (0.01 mol L–1) in the presence of the
ferroin indicator to determine the exact H2O2 initial concentration. The autoclave was pressurized with 2.9 MPa
5% H2/CO2, cooled down to 2 °C, and then
stirred for 0.5 h (1200 rpm). H2O2 degradation
activity (molH2O2 kgcat–1 h–1) was determined by titrating aliquots (ca. 0.05 g) of the final filtrated solution after the reaction with
acidified Ce(SO4)2 (8.5 mmol L–1) in the presence of the ferroin indicator. For the in situ acid addition tests, 5.4 × 10–4 g of acid
(H2SO4, 98 wt %) was added as a 2 wt % aqueous
solution. H2O2 degradation activity (molH2O2 mmol–1metal h–1) was normalized with respect to the actual metal loading determined
by MP-AES.
Paris C.B., Howe A.G., Lewis R.J., Hewes D., Morgan D.J., He Q, & Edwards J.K. (2022). Impact of the Experimental Parameters on Catalytic Activity When Preparing Polymer Protected Bimetallic Nanoparticle Catalysts on Activated Carbon. ACS Catalysis, 12(8), 4440-4454.
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7

Doxycycline Quantification in Tear Samples

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Doxycycline hyclate (Sigma-Aldrich ≥98% HPLC; Sigma-Aldrich, St Louis, MO) was prepared as solutions of 100 and 1,000 ng/mL (representative of low and high tear concentrations obtained in the present in vivo experiment) in a buffer made of 50% acetonitrile (HPLC grade; Fisher Scientific, Raleigh, NC), 50% water (HPLC grade; Fisher Scientific, Raleigh, NC), and 0.1% formic acid. Twenty Schirmer strips (wet until the 20-mm mark was reached) and 20 PVA sponges (spiked with 25 μL, representative of the median volume absorbed by these sponges in the present in vivo experiment) were used for each doxycycline concentration, 10 of which underwent extraction with centrifugation while the remaining 10 underwent elution with methanol, following the same protocol as the in vivo experiment. Samples were then stored at −80°C until analysis.
Sebbag L., Showman L., McDowell E.M., Perera A, & Mochel J.P. (2018). Impact of Flow Rate, Collection Devices, and Extraction Methods on Tear Concentrations Following Oral Administration of Doxycycline in Dogs and Cats. Journal of Ocular Pharmacology and Therapeutics, 34(6), 452-459.
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8

Synthesis and Characterization of Polymers

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Humulin R (Eli Lilly) was purchased and used as received. Solvents N,N-dimethylformamide (DMF; HPLC Grade, Alfa Aeser, >99.7%), hexanes (Fisher, Certified ACS, >99.9%), ether (Sigma, Certified ACS, Anhydrous,>99%) and CDCl3 (Acros, >99.8%) were used as received. Monomers N-(3-methoxypropyl)acrylamide (MPAM; Sigma, 95%), 4-acryloylmorpholine (MORPH; Sigma, >97%) were filtered with basic alumina prior to use. Monomers N-phenylacrylamide (PHE; Sigma, 99%) and N-isopropylacrylamide (NIPAM; Sigma, >99%) were used as received. RAFT chain transfer agents 2-cyano-2-propyl dodecyl trithiocarbonate (2-CPDT; Strem Chemicals, >97%) and 4-((((2-carboxyethyl)thio)carbonothioyl)thio)-4-cyanopentanoic acid (BM1433; Boron Molecular, >95%) were used as received. Initiator 2,2’-azobis(2-methyl-propionitrile) (AIBN; Sigma, >98%) was recrystallized from methanol (MeOH; Fisher, HPLC Grade, >99.9%) and dried under vacuum before use. Z-group removing agents lauroyl peroxide (LPO; Sigma, 97%) and hydrogen peroxide (H2O2; Sigma, 30%) were used as received. Streptozotocin (99.58%) was purchased from MedChem Express. All other reagents were purchased from Sigma-Aldrich unless otherwise specified.
Maikawa C.L., Mann J.L., Kannan A., Meis C.M., Grosskopf A.K., Ou B.S., Autzen A.A., Fuller G.G., Maahs D.M, & Appel E.A. (2021). Engineering Insulin Cold Chain Resilience to Improve Global Access. Biomacromolecules, 22(8), 3386-3395.
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9

HPLC Analysis of Compounds

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All analyses were performed using a Shimadzu Prominence series HPLC system (Shimadzu Corporation, Kyoto, Japan), equipped with a LC-20AB binary pump (Serial: L20124200883), SIL-20A HT autosampler (Serial: L20345256104), CTO-20AC temperature controlled column oven (Serial: L2021525077), SPD-M20A photodiode array detector and CBM-20A communications bus (Serial: L20235154327). All equipment was controlled by Shimadzu Lab Solutions Lite software version 5.71 SP2. For separation, an Ultra C18 column, 3 μm, 150 × 4.6 mm (RESTEK Corporation, Bellefonte, PA, USA) was used. Samples were analyzed at a constant solvent flow rate of 1.0 mL/min at 35 °C using a binary gradient (Table 1). Solvent A consisted of a ddH2O (0.2 μm filtered) and solvent B consisted of 20% ddH2O in acetonitrile (HPLC grade, Fisher Scientific, Fairlawn, NJ, USA) with each solvent containing 0.18% formic acid (v/v, LC/MS grade) and 0.15% triethylamine (v/v, HPLC grade, Fisher Scientific, Fairlawn, NJ, USA).
Fraleigh N.L., Lewicky J.D., Martel A.L., Diaz-Mitoma F, & Le H.T. (2021). Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine. Vaccines, 9(2), 118.
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10

Flufenamic Acid Extraction and Purification

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Flufenamic acid (FFA) (Sigma Aldrich, 97%), ethanol (Fisher Scientific, HPLC grade), water (Fisher Scientific, HPLC grade).
Cookman J., Hamilton V., Hall S.R, & Bangert U. (2020). Non-classical crystallisation pathway directly observed for a pharmaceutical crystal via liquid phase electron microscopy. Scientific Reports, 10, 19156.
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