Tert amyl alcohol

Puerarin was purchased from Alibaba Co., Ltd. (Hangzhou, China). Lauric acid, tetrahydrofuran (THF), and acetonitrile were purchased from Junsei (Tokyo, Japan), while myristic acid, palmitic acid, stearic acid, oleic acid, 1,4-dioxane, acetone, tert-butyl alcohol, tert-amyl alcohol, 1,2-dichloroethane, chloroform, and n-heptane were purchased from Dae-Jung (Sigeung-si, Republic of Korea). In addition, palmitic anhydride, vinyl palmitate, ethyl palmitate, butyl palmitate, 1-octanol, and ABTS were obtained from TCI (Tokyo, Japan), and methyl palmitate was purchased from Aladdin (Shanghai, China). Ethyl acetate and dimethyl sulfoxide-d₆ (DMSO-d₆) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Commercially used immobilized enzymes, Novozym 435 (Candida antarctica lipase B immobilized on acrylic resin), Lipozyme TL IM (Thermomyces lanuginosus immobilized on a silica gel carrier), and Lipozyme RM IM (Rhizomucor miehei immobilized on a resin carrier) were obtained from Novozymes (Bagsværd, Demark). The acetic acid, acetonitrile, methanol, and water used for HPLC analysis were purchased from J.T.Baker (Phillipsburg, NJ, USA) in HPLC grade. Formic acid used for LC–MS analysis was purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Naringin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid, acetic anhydride, mEthyl acetate, vinyl acetate, propyl acetate, butyl acetate, acetone, tert-butyl alcohol, tert-amyl alcohol, and 1,2-dichloroethane were purchased from Dae-Jung (Gyeonggi-do, Korea). Ethyl acetate, acetonitrile, and tetrahydrofuran (THF) were purchased from Junsei (Tokyo, Japan). For the immobilized enzyme, commercially used Novozym 435 (Candida antarctica lipase B immobilized on acrylic resin), Lipozyme TL IM (Thermomyces lanuginosus immobilized on a silica gel carrier), and Lipozyme RM IM (Rhizomucor miehei immobilized on a resin carrier) were selected, purchased from Novozymes (Bagsværd, Demark).

Naringin (95%) and ethyl acetate (99.8%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Novozym 435 (immobilized Candida antarctica lipase B), Lipozyme TL IM (immobilized Thermomyces lanuginosus), and Lipozyme RM IM (immobilized Rhizomucor miehei) were purchased from Novo Nordisk Bioindustry (Bagsvaerd, Denmark). Dimethyl sulfoxide (DMSO) (99.5%), 1,4-dioxane (99.5%), acetone (99.5%), tert-butanol (99.5%), tert-amyl alcohol (99%), 1,2-dichloroethane (99%), isooctane (98%), oleic acid (99%), and molecular sieves (3-Å) were purchased from Dae-Jung Chemical and Metals Co. Ltd. (Gyunggido, Korea). Acetonitrile (99.5%) and tetrahydrofuran (THF) (99.5%) were purchased from Junsei Chemical Co. Ltd. (Tokyo, Japan). Methanol, water, and acetic acid (HPLC grade) were purchased from J.T. Baker (Phillipsburg, NJ, USA).