Beh c18

Manufactured by Waters Corporation

Sourced in United States, Germany, Ireland, China

The BEH C18 is a high-performance liquid chromatography (HPLC) column developed by Waters Corporation. The column features a bidentate C18 stationary phase chemistry that provides enhanced stability and reproducibility for reversed-phase separations. The BEH C18 column is designed to deliver consistent and reliable performance for a wide range of analytical applications.

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XBridge BEH C18 OBD Prep Column (9 citations) XBridge BEH C18 XP Column (5 citations) BEH C18 trapping columns (5 citations) BEH 130 C18 analytical column (4 citations) XBridge Oligonucleotide BEH C18 column (4 citations) VanGuard BEH C18 (4 citations) Xbridge UPLC BEH C18 precolumn (3 citations) NanoACQUITY 1.7 μm BEH C18 column (3 citations) X-Bridge BEH 130 C18 sorbent (3 citations) BEH C18 150 × 2.1 mm column (2 citations)

162 protocols using beh c18

Targeted Proteomics Using TMTc+ on Orbitrap Fusion

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The instrument was equipped with an Easy nLC 1200 high pressure liquid chromatography (HPLC) pump. For each run, peptides were separated on a 100 mm inner diameter microcapillary column, packed first with approximately 0.5 cm of 5mm BEH C18 packing material (Waters) followed by 30 cm of 1.7mm BEH C18 (Waters). Separation was achieved by applying a 6%–30% ACN gradient in 0.125% formic acid and 2% DMSO over 90 min at 350 nL/min at 60°C. Electrospray ionization was enabled by applying a voltage of 2.6 kV through a microtee at the inlet of the microcapillary column.
The Orbitrap Fusion Lumos used the TMTc+ method (Sonnett et al., 2018 (link)). The mass spectrometer was operated in data-dependent mode with a survey scan ranging from 500–1400 m/z at resolution of 120k (200 m/z). 10 most intense ions for CID MS2 fragmentation using the quadrupole. Only peptides of charge state 2+ were included. Dynamic exclusion range was set to 60 s with mass tolerance of 10ppm. Selected peptides were fragmented using 32% HCD collision energy, and the resultant MS2 spectrum was acquired using the Orbitrap with a resolution of 60k and 0.4 Th isolation window.

Cao W.X., Kabelitz S., Gupta M., Yeung E., Lin S., Rammelt C., Ihling C., Pekovic F., Low T.C., Siddiqui N.U., Cheng M.H., Angers S., Smibert C.A., Wühr M., Wahle E, & Lipshitz H.D. (2020). Precise Temporal Regulation of Post-transcriptional Repressors Is Required for an Orderly Drosophila Maternal-to-Zygotic Transition. Cell reports, 31(12), 107783.

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Rapid Peptide Identification via Online Digestion

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PhosB was solubilized in equilibration
buffer (10 mM potassium phosphate, pH 7.0) and diluted 1:1 (vol/vol)
with quench buffer (100 mM potassium phosphate, pH 2.3). The HDX manager
of the nanoACQUITY system was equipped with a Vanguard column (BEH
C18, 130 Å, 1.7 μm, 2.1 mm × 5 mm; Waters) and an
Acquity UPLC column (BEH C18, 130 Å, 1.7 μm, 1.0 mm ×
100 mm; Waters) for peptide trapping and separation, respectively.
Protein digestion was performed online with the UPLC chromatographic
system using an in-house packed protease column (immobilized pepsin
agarose resin) at 15 °C. The generated peptides were trapped
and washed with solvent A at 200 μL/min for 3 min. Subsequently,
peptides were separated by applying a 7.5 min linear gradient from
8 to 35% solvent B at 40 μL/min. Peptides were measured in positive
ion mode between 50 and 2000 m/z on the Xevo G2-XS mass spectrometer.
Experiments were performed in triplicate on the standard two-valve
and extended three-valve configurations applying a standard bottom-up
HDX-MS workflow. Detailed LC settings are provided in the Supporting
Information (Tables S3 and S5).

Hammerschmid D., Calvaresi V., Bailey C., Russell Lewis B., Politis A., Morris M., Denbigh L., Anderson M, & Reading E. (2023). Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein–Lipid Assemblies. Analytical Chemistry, 95(5), 3002-3011.

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Preparative LC/MS Purification of Compound

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Example 4131

The crude material was purified via preparative LC/MS with the following conditions: Column: XBridge C18, 19×200 mm, 5-μm particles; Mobile Phase A: 5:95 acetonitrile:water with 10-mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 10-mM ammonium acetate; Gradient: 15-55% B over 20 minutes, then a 5-minute hold at 100% B; Flow: 20 mL/min. Fractions containing the desired product were combined and dried via centrifugal evaporation. Two LCMS were used to determine purity. Injection1: Column: Waters BEH C18, 2.0×50 mm, 1.7 μm; Mobile Phase A: 5:95 ACN:H2O with 10 mM NH4OAc; Mobile Phase B: 95:5 ACN:H2O with 10 mM NH4OAc; Temperature: 50° C.; Gradient: 0-100% B over 3 min, then a 0.75 min hold at 100% B; Flow: 1 mL/min. Injection2: Column: Waters BEH C18, 2.0×50 mm, 1.7-μm; Mobile Phase A: 5:95 MeOH:H2O with 10 mM NH4OAc; Mobile Phase B: 95:5 MeOH:H2O with 10 mM NH4OAc; Temperature: 50° C.; Gradient: 0-100% B over 3.5 min, then a 0.5-min hold at 100% B; Flow: 0.5 mL/min.

LCMS (Injection 1 conditions) Rt=1.46 min, ESI m/z 715.1 (M+H).

LCMS (Injection 2 conditions) Rt=1.39 min, ESI m/z 715.1 (M+H).

US10745382B2. Compounds useful as immunomodulators (2020-08-18). BRISTOL-MYERS SQUIBB COMPANY [US]. Inventors: Kap-Sun Yeung [US], Timothy P. Connolly [US], David B. Frennesson [US], Katharine A. Grant-Young [US], Piyasena Hewawasam [US], David R. Langley [US], Zhaoxing Meng [US], Eric Mull [US], Kyle E. Parcella [US], Mark George Saulnier [US], Li-Qiang Sun [US], Alan Xiangdong Wang [US], Ningning Xu [US], Juliang Zhu [US], Paul Michael Scola [US].

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Targeted Proteomics Using TMTc+ on Orbitrap Fusion

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UHPLC-Orbitrap Velos Pro Mass Spectrometry

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All experiments were performed on a Thermo Scientific Accela UHPLC system with an Accela 600 Pump, Accela Autosampler and Accela PDA Detector coupled to a Thermo Fisher Scientific LTQ Orbitrap Velos Pro mass spectrometer (Thermo Scientific, Waltham, MA, USA). All samples were centrifuged using a 5424R Eppendorf centrifuge (Eppendorf, Hamburg, Germany). All ACQUITY UPLC columns (BEH C18 (2.1 mm × 100 mm, 1.7 μm, 130 Å), BEH Phenyl (2.1 mm × 100 mm, 1.7 μm, 130 Å) and CSH Phenyl-Hexyl (2.1 mm × 100 mm, 1.7 μm, 130 Å)) and all ACQUITY UPLC VanGuard pre-columns (BEH C18 (2.1 mm × 5 mm, 1.7 μm, 130 Å), BEH Phenyl (2.1 mm × 5 mm, 1.7 μm, 130 Å) and CSH Phenyl-Hexyl (2.1 mm × 5 mm, 1.7 μm, 130 Å)) were purchased from Waters (Milford, MA, USA).

Prothmann J., Spégel P., Sandahl M, & Turner C. (2018). Identification of lignin oligomers in Kraft lignin using ultra-high-performance liquid chromatography/high-resolution multiple-stage tandem mass spectrometry (UHPLC/HRMSn). Analytical and Bioanalytical Chemistry, 410(29), 7803-7814.

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Purification and Characterization of Isomeric Compound

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Example 6

[Figure (not displayed)]

The yield of the second eluting isomer was 3.2 mg, and its estimated purity by LCMS analysis was 98%. Two analytical LC/MS injections were used to determine the final purity. Injection 1 conditions: Column: Waters BEH C18, 2.0×50 mm, 1.7-μm particles; Mobile Phase A: 5:95 acetonitrile:water with 10 mM ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 10 mM ammonium acetate; Temperature:50° C.; Gradient: 0% B, 0-100% B over 3 minutes, then a 0.5-minute hold at 100% B; Flow: 1 mL/min; Detection: UV at 220 nm. Retention time: 2.23; M+H=593. Injection 2 conditions: Column: Waters BEH C18, 2.0×50 mm, 1.7-μm particles; Mobile Phase A: 5:95 methanol:water with 10 mM ammoniumacetate; Mobile Phase B: 95:5 methanol:water with 10 mM ammonium acetate; Temperature: 50° C.; Gradient: 0% B, 0-100% B over 3 minutes, then a 0.5-minute hold at 100% B; Flow: 0.5 mL/min; Detection: UV at 220 nm. Retention time: 3.14; M+H=593. Proton NMR was acquired in deuterated DMSO. ¹H NMR (500 MHz, DMSO-d6) δ 8.36 (s, 1H), 8.29 (s, 1H), 7.81 (d, J=7.7 Hz, 1H), 7.74 (s, 1H), 7.44 (t, J=7.7 Hz, 1H), 7.32-7.20 (m, 5H), 7.08 (d, J=11.7 Hz, 1H), 4.97 (s, 1H), 4.29-4.15 (m, 2H), 2.72-2.60 (m, 2H), 2.32 (s, 3H), 2.23 (s, 3H), 2.08-2.00 (m, 2H), 1.79 (s, 3H), 0.90 (s, 9H).

US09938271B2. Inhibitors of human immunodeficiency virus replication (2018-04-10). VIIV HEALTHCARE UK (NO. 5) LIMITED [GB]. Inventors: Kyle J. Eastman [US], John F. Kadow [US], B. Narasimhulu Naidu [US], Kyle E. Parcella [US], Kevin Peese [US].

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Sulfadiazine Quantification and Degradation Analysis

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Ultra-performance liquid chromatography (UPLC)-triple quadrupole mass spectrometry (Waters Xevo TQs, USA) with a chromatographic column (BEH C18, 1.7 μm, 2.1 × 50 mm, Waters ACQUITY UPLC) was used to determine the concentration of sulfadiazine. Positive polarity mode with electrospray ionization was used for sulfadiazine determination. The column temperature was maintained at 30 °C. The mobile phase was a mixture of 2:8 deionized water (0.5% formic acid) / acetonitrile (0.5% formic acid) (v/v) with flow rate of 0.30 mL/min.
Sulfadiazine degradation product analysis was conducted using UPLC/QTOF (Waters Xevo G2 QTOF, USA) with a chromatographic column (BEH C18, 1.7 μm, 2.1 × 50 mm, Waters ACQUITY UPLC). Accurate MS^E mode was used to analyze sulfadiazine and the degradation products with a scan range of 50–650 m/z.

Xin X., Liu H., Sun J., Gao K, & Jia R. (2023). Enhanced photocatalytic activity of Fe-, S- and N-codoped TiO2 for sulfadiazine degradation. International Journal of Environmental Science and Technology.

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LC-MS/MS Analysis of Secretome Peptides

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LC-MS/MS analysis of the tryptic peptides derived from the depleted secretome samples was performed essentially as described above for the intracellular proteome, except different types of columns were used for the analyses. Peptides (500 ng) were loaded at 1% B at a flow rate of 5 µL/min for 3 min onto the precolumn (BEH C18, 180 μm × 20 mm, 5 µm; Waters, Milford, MA, USA), and then separated using a 60 min gradient from 5–35% B on a 25 cm column (BEH C18, 25 cm × 75 µm, 1.7 µm; Waters, Milford, MA, USA). Each of the three depletion replicates were analyzed three times (injection triplicates).

Tascher G., Burban A., Camus S., Plumel M., Chanon S., Le Guevel R., Shevchenko V., Van Dorsselaer A., Lefai E., Guguen-Guillouzo C, & Bertile F. (2019). In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes. Cells, 8(2), 192.

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UPLC Method for Compound Separation

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Analytical UPLC runs were done using a Waters (Eschborn, Germany) Acquity system consisting of a sample manager FTN, quaternary solvent manager, PDA detector, column manager and column manager aux. The following Waters Acquity UPLC^® columns were used: BEH C4 2.1 × 100 mm 300 Å 1.7 μm, BEH C8 2.1 × 100 mm 130 Å 1.7 μm, BEH C18 2.1 × 50 mm 130 Å 1.7 μm and BEH C18 2.1 × 100 mm 300 Å 1.7 μm. Separation was achieved using a gradient of solvent A (0.1% TFA in H₂O) and solvent B (0.1% TFA in ACN) from 5% B to 95% B in 2.5 min at a flowrate of 0.613 ml/min for 50 mm columns or in 5 min at a flowrate of 0.5 ml/min for 100 mm columns.

Pech A., Achenbach J., Jahnz M., Schülzchen S., Jarosch F., Bordusa F, & Klussmann S. (2017). A thermostable d-polymerase for mirror-image PCR. Nucleic Acids Research, 45(7), 3997-4005.

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Quantification of Patulin in Samples by HPLC-MS

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HPLC/MS (ACQUITY ^® TQD, Waters, Milford, MA, USA) was used to detect and quantify patulin in the samples. The chromatographic separation was achieved on a C₁₈ BEH (2.1 × 100 mm, 1.7 μm) (Waters, Milford, MA, USA) column at 30 °C with a flow rate of 0.3 mL/min. The injection was made with a volume of 5 µL. Eluent A was HPLC-grade water and eluent B was acetonitrile (HPLC MS grade, Sigma-Aldrich, St. Louis, MO, USA).
Elution conditions were as follows: a 1 min isocratic passage of solvent B from 100 to 2%, a 3 min gradient increase of solvent B from 2 to 90%, 1 min isocratic passage of solvent B at 90%, a 1 min gradient decrease of solvent B from 90 to 2 and re-equilibration at 2% solvent B for 4 min.
Electrospray ionization was the ionization source. The parameters were as follows: Source temperature: 120 °C, desolvation temperature: 300 °C, Cone gas: 60 L/h, desolvatation gas flow: 800 L/h. Spectra were acquired in negative ionization selected reaction monitoring (SIR) mode with interchannel delay of 0.050 s.

Settier-Ramírez L., López-Carballo G., Hernández-Muñoz P., Fontana A., Strub C, & Schorr-Galindo S. (2021). New Isolated Metschnikowia pulcherrima Strains from Apples for Postharvest Biocontrol of Penicillium expansum and Patulin Accumulation. Toxins, 13(6), 397.

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