Human normal colonic epithelial cell line CCD-18Co, human colorectal adenocarcinoma cell lines (LS174T, HCT116, HCT-15, SW480, LOVO), and human renal epithelial cell line 293T were purchased from American Type Culture Collection (ATCC, USA). The above cells were kept in DMEM/F12 medium (Gibco, USA) containing 10% fetal bovine serum (FBS).
Human NK cell line NK92 was bought from ATCC and cultured in Minimum Essential Medium α (MEMα) containing 10% horse serum, 10% FBS, 0.1 mM β-mercaptoethanol, and 1% penicillin-streptomycin in an incubator (37°C, 5% CO2) [14 (link)]. To activate NK92 cells, they were stimulated with 100 U/ml IL-2 (BD Biosciences, USA) for 24 h.
The oe-E2F7 vector, si-RAD18#1 vector, si-RAD18#2 vector, si-E2F7#1 vector, and si-E2F7#1 vector were purchased from RiboBio (China). Plasmids were transfected into the corresponding CRC cells utilizing the Lipofectamine 2000 kit (Thermo Fisher Scientific, USA).
Human NK cell line NK92 was bought from ATCC and cultured in Minimum Essential Medium α (MEMα) containing 10% horse serum, 10% FBS, 0.1 mM β-mercaptoethanol, and 1% penicillin-streptomycin in an incubator (37°C, 5% CO2) [14 (link)]. To activate NK92 cells, they were stimulated with 100 U/ml IL-2 (BD Biosciences, USA) for 24 h.
The oe-E2F7 vector, si-RAD18#1 vector, si-RAD18#2 vector, si-E2F7#1 vector, and si-E2F7#1 vector were purchased from RiboBio (China). Plasmids were transfected into the corresponding CRC cells utilizing the Lipofectamine 2000 kit (Thermo Fisher Scientific, USA).