Crc 25 dose calibrator

Manufactured by Mirion Technologies

The CRC-25 Dose Calibrator is a laboratory instrument designed for the accurate measurement of radioactivity. It provides precise quantification of the activity levels of various radiopharmaceuticals and radioactive samples. The device's core function is to determine the amount of radioactivity present in a given sample, enabling users to ensure the correct dosage for medical procedures or research applications.

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Lab products found in correlation

Itlc sa, by Agilent Technologies (4 mentions) Amersham typhoon bioimager, by GE Healthcare (4 mentions) Imagequant software, by GE Healthcare (4 mentions) Wizard 2480 gamma counter, by PerkinElmer (4 mentions) Microflex lrf, by Bruker (3 mentions) Nanodrop one microvolume uv vis spectrophotometer, by Thermo Fisher Scientific (2 mentions) Nanodrop onec microvolume uv vis spectrophotometer, by Thermo Fisher Scientific (2 mentions) Ph indicator paper, by Merck Group (1 mentions) Integral 5 water purification system, by Merck Group (1 mentions)

Close spelling variants of this product

CRC-25 PET dose calibrator Dose calibrator

7 protocols using crc 25 dose calibrator

Radiochemical Synthesis and Stability

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All reagents were purchased from Thermo
Fisher Scientific unless otherwise stated and used without further
purification. Water was deionized using a Select Fusion ultrapure
water deionization system (Suez) and had a resistance of >18.2
MΩ
cm^–1 at 25 °C. Protein concentration measurements
were obtained using a NanoDrop One Microvolume UV–vis spectrophotometer
(NanoDrop Technologies, Inc.). Mass spectrometry measurements were
performed using a Thermo RSLC coupled to Bruker maXis. Radioactivity
measurements were obtained using a CRC-25 Dose Calibrator (Capintec,
Inc.) or a Wizard 2480 Gamma Counter (PerkinElmer). RIC synthesis
and serum stability studies were monitored by instant thin-layer chromatography
using glass microfiber chromatography paper (iTLC-SA, Agilent). Radio-iTLC
strips were measured by autoradiography (Amersham Typhoon Bioimager,
GE) and analyzed using ImageQuant software (GE Healthcare). All experiments
were performed in accordance with the United Kingdom Human Tissue
Act (2004) regulations. Appropriate informed consent for the use of
human tumor specimen slides and paraffin embedded blocks was obtained
and approved by the Newcastle and North Tyneside 1 Research Ethics
Committee (REC reference number: 17/NE/0361).

Pringle T.A., Chan C.D., Luli S., Blair H.J., Rankin K.S, & Knight J.C. (2022). Synthesis and In Vivo Evaluation of a Site-specifically Labeled Radioimmunoconjugate for Dual-Modal (PET/NIRF) Imaging of MT1-MMP in Sarcomas. Bioconjugate Chemistry, 33(8), 1564-1573.

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Radioimmunoconjugate Synthesis and Stability

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All reagents were purchased from Fisher Scientific unless otherwise stated and used without further purification. Water was deionized using a Select Fusion ultrapure water deionisation unit (Suez) and had a resistance of >18.2 MΩ cm⁻¹ at 25 °C. Protein concentration measurements were obtained using a NanoDrop One^C Microvolume UV-vis Spectrophotometer (NanoDrop Technologies, Inc.). MALDI-TOF mass spectrometry measurements were taken on a Bruker Microflex LRF. Radioactivity measurements were obtained using a CRC-25 Dose Calibrator (Capintec, Inc.) or a Wizard 2480 Gamma Counter (PerkinElmer). Radioimmunoconjugate synthesis and serum stability studies were monitored by instant thin-layer chromatography using glass microfiber chromatography paper (iTLC-SA, Agilent). Radio-iTLC strips were measured by autoradiography (Amersham Typhoon Bioimager, GE) and analysed using ImageQuant software (GE Healthcare). pH measurements were determined using pH indicator paper (Merck Millipore) or a pH Spear electrode (Eutech Instruments).

Pringle T.A., Coleman O., Kawamura A, & Knight J.C. (2022). The influence of degree of labelling upon cellular internalisation of antibody-cell penetrating peptide conjugates. RSC Advances, 12(43), 27716-27722.

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Biodistribution of Radioisotope-Labeled Exosomes

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Nude mice were injected with 130–140 µL of [¹¹¹In]PC3-exosomes (7.6 µCi, 30 µg), [¹¹¹In]MCF7-exosomes (7.2 µCi, 32 µg), or [¹¹¹In]liposomes (6.1 µCi, 34 µg) in the tail vein. The syringes were measured in a CRC 25 dose calibrator (Capintec, Ramsey, NY) before and after injection. A standard of 0.76 µCi [¹¹¹In]Cl₃ was prepared at the time of injection and diluted to 1 ml. Serial blood samples (25–30 µL) were collected from the mice at 0.5, 1.5, 3, and 7 hours post injection by the modified tail clip method [34 (link)]. After 24 hours, the whole body retention of ¹¹¹In in the mice was measured in a dose calibrator and the mice were sacrificed by CO₂ asphyxiation followed by cervical dislocation. Blood samples were immediately drawn by cardiac puncture and the mice were subjected to a full necropsy. Samples of all organs were collected, weighed, and counted in a 2480 Wizard gamma counter (Perkin Elmer, Waltham, MA) along with the blood samples and ¹¹¹In standard. The gamma counter data was converted to a percentage of the injected dose (%ID) by considering the known activity injected and reference standard activity and the cpm of the organ and reference standard. The blood clearance data is expressed as %ID in blood. The total weight of blood in mice is estimated to be 5.5 ml/100 g [35 ]. Biodistribution data is expressed as a %ID/g of organ tissue.

Smyth T., Kullberg M., Malik N., Smith-Jones P., Graner M.W, & Anchordoquy T.J. (2014). Biodistribution and Delivery Efficiency of Unmodified Tumor-Derived Exosomes. Journal of controlled release : official journal of the Controlled Release Society, 199, 145-155.

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Radioiodine Blood Activity Measurement

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Blood activity was measured in a calibrated well counter (Capintec CRC-25 Dose Calibrator) with precision of microcurie. Background count was measured without blood samples before measurement of each blood sample activity. Two milliliter blood samples were collected at different time periods postadministration of radioiodine. The 1st measurement was performed approximately 2 hours after radioiodine administration, with further measurements performed after 6, 12, 24, 48, and 96 hours. Similarly, along with whole body measurements, the patients were asked to take the 1st blood sample without maturation, 2 hours after radioiodine administration. Data accuracy was confirmed by quality control methods as recommended in Lassmann et al.^{2 (link)} All blood activity was normalized to the administrated activity to calculate the retention per mL of blood.

Piruzan E., Haghighatafshar M., Faghihi R, & Entezarmahdi S.M. (2016). Calculation of Blood Dose in Patients Treated With 131I Using MIRD, Imaging, and Blood Sampling Methods. Medicine, 95(11), e3154.

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Radioimmunoconjugate Synthesis and Evaluation

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All reagents were purchased from Thermo Fisher Scientific unless otherwise stated and used without further purification. Water was deionized using a Select Fusion ultrapure water deionisation system (Suez) and had a resistance of > 18.2 MΩ cm^–1 at 25 °C. Protein concentration measurements were obtained using a NanoDrop One Microvolume UV–Vis Spectrophotometer (NanoDrop Technologies, Inc.). MALDI-TOF mass spectrometry measurements were taken on a Bruker Microflex LRF. Radioactivity measurements were obtained using a CRC-25 Dose Calibrator (Capintec, Inc.) or a Wizard 2480 Gamma Counter (PerkinElmer). Radioimmunoconjugate synthesis and serum stability studies were monitored by instant thin-layer chromatography using glass microfiber chromatography paper (iTLC-SA, Agilent). Radio-iTLC strips were measured by autoradiography (Amersham Typhoon Bioimager, GE) and analysed using ImageQuant software (GE Healthcare).

Pringle T.A., Ramon-Gil E., Leslie J., Oakley F., Wright M.C., Knight J.C, & Luli S. (2024). Synthesis and preclinical evaluation of a 89Zr-labelled human single chain antibody for non-invasive detection of hepatic myofibroblasts in acute liver injury. Scientific Reports, 14, 633.

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Radiolabeling Immunoconjugate Protocol

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All materials were obtained from Fisher
Scientific unless otherwise specified and used without any further
purification. Deionized water was obtained using a Select Fusion ultrapure
water deionization system (Suez) with a resistance of >18.2 MΩ/cm
at 25 °C. Absorbance measurements were obtained using a NanoDrop
One^C Microvolume UV–vis spectrophotometer (NanoDrop
Technologies, Inc.). MALDI-TOF mass spectrometry analysis was performed
by using a Bruker Microflex LRF. Radioactivity measurements were obtained
by using a CRC-25 dose calibrator (Capintec, Inc.). Radiolabeling
of immunoconjugates was verified by instant thin-layer chromatography
(iTLC) using glass microfiber chromatography paper (iTLC-SA, Agilent).
Autoradiography of radio-iTLC strips was imaged using an Amersham
Typhoon bioimager (GE) and analyzed using ImageQuant software (GE
Healthcare).

Gristwood K., Luli S., Rankin K.S, & Knight J.C. (2023). Synthesis and In Vitro Evaluation of a HER2-Specific ImmunoSCIFI Probe. ACS Omega, 8(50), 47905-47912.

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Radionuclide Safety Protocols

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All experiments involving the use of radioactive materials at Washington University are conducted under the authorization of the Radiation Safety Committee in accordance with the University's Nuclear Regulatory Commission license. Activity was determined with a Capintec CRC-25 Dose Calibrator calibrated to a factor of 465 for ⁸⁹Zr (Capintec, Ramsey, NJ). All chemicals were purchased from Sigma-Aldrich (St. Louis, MO), unless otherwise specified, and all solutions were prepared using ultrapure water with an 18 MΩ-cm resistivity produced by a Millipore Integral 5 water purification system (Millipore, Billerica, MA).

Lange S.E., Zheleznyak A., Studer M., O'Shannessy D.J., Lapi S.E, & Van Tine B.A. (2016). Development of 89Zr-Ontuxizumab for in vivo TEM-1/endosialin PET applications. Oncotarget, 7(11), 13082-13092.

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