Mouse anti human iga2

Manufactured by Southern Biotech

The Mouse anti-human IgA2 is a laboratory reagent used to detect and quantify the presence of IgA2 immunoglobulin in biological samples. It is a monoclonal antibody produced in mice that specifically binds to the human IgA2 subclass.

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Lab products found in correlation

Mouse anti human iga1, by Southern Biotech (2 mentions) Goat f ab 2 anti human iga, by Southern Biotech (1 mentions)

Spelling variants of this product

Anti‐human IgA2 (2 citations) Mouse anti-human IgA2 Alexa Fluor 647 (2 citations)

2 protocols using mouse anti human iga2

Serum IgA Subclass Levels in Rheumatoid Arthritis

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Sera were obtained from patients with established RA presenting at the Department of Medicine 3 of the University Clinic of Erlangen and of age- and sex-matched healthy volunteers. Disease activity was evaluated using the DAS28 score. Concentrations of total serum IgA, IgA1, and IgA2 was determined by ELISA using goat F(ab)₂ anti-human IgA (#2052-01) as capture and HRP-coupled goat anti-IgA (#2050-05), mouse anti-human IgA1 (#9130-05), or mouse anti-human IgA2 (#9140-05) as detection antibodies (all Southern Biotech). ACPA levels were determined using cyclic-citrullinated peptide-coated plates (Orgentec Diagnostika).

Steffen U., Koeleman C.A., Sokolova M.V., Bang H., Kleyer A., Rech J., Unterweger H., Schicht M., Garreis F., Hahn J., Andes F.T., Hartmann F., Hahn M., Mahajan A., Paulsen F., Hoffmann M., Lochnit G., Muñoz L.E., Wuhrer M., Falck D., Herrmann M, & Schett G. (2020). IgA subclasses have different effector functions associated with distinct glycosylation profiles. Nature Communications, 11, 120.

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Detecting IgA HLA Antibodies: Optimized Assays

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For IgA HLA antibody detection, the following detection antibodies were used: mouse anti‐human IgA1 (clone: B3506B4), mouse anti‐human IgA2 (clone: A9604D2), goat anti‐human pan IgA‐PE, heavy chain‐specific (all from Southern Biotech, Birmingham, AL), and mouse anti‐human IgA2 (clone: IS11‐21E11, MACS Miltenyi Biotec). For optimization and validation of the IgA‐LMX and IgA‐SAB assay, the following in‐house developed HLA‐specific mAbs were used: human recombinant ‐IgA1 and ‐IgA2 mAbs derived from the previously described MUS4H4‐IgG1, recognizing HLA‐Bw4/A24/A32/A25 and B cell hybridoma derived human mAbs (IgG, SN607D8 recognizing A2/A28 and IgM blend, which is a mixture of 10 different human mAbs of the IgM isotype together recognizing the complete panel of HLA‐class I coated beads included in the SAB panel).²⁴, ²⁵

Car H., Karahan G.E., Dreyer G.J., Brand‐Schaaf S.H., de Vries A.P., van Kooten C., Kramer C.S., Roelen D.L., Claas F.H, & Heidt S. (2020). Low incidence of IgA isotype of HLA antibodies in alloantigen exposed individuals. Hla, 97(2), 101-111.

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