Synapsin

Manufactured by Abcam

Sourced in Germany

SYNAPSIN is a family of phosphoproteins found in the presynaptic terminals of neurons. They are involved in the regulation of neurotransmitter release and the organization of the cytoskeleton at the synapse.

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Lab products found in correlation

Ab18259, by Abcam (1 mentions) Ab21990, by Abcam (1 mentions) Ab195045, by Abcam (1 mentions) Ab31940, by Abcam (1 mentions) A2052, by Merck Group (1 mentions) Ab1511, by Merck Group (1 mentions) Nestin, by BD (1 mentions) Confocal microscope, by Leica (1 mentions) Map2b, by Merck Group (1 mentions) βiii tubulin, by Fortrea (1 mentions) Ab254349, by Abcam (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Psd 95, by Santa Cruz Biotechnology (1 mentions) Glur1, by Santa Cruz Biotechnology (1 mentions) Tiam1, by Santa Cruz Biotechnology (1 mentions) Sc 514583, by Santa Cruz Biotechnology (1 mentions) Sc 13152, by Santa Cruz Biotechnology (1 mentions) Sc 32290, by Santa Cruz Biotechnology (1 mentions) Sc 393315, by Santa Cruz Biotechnology (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

Spelling variants of this product

Synapsin-1 (19 citations) Anti-synapsin 1 (12 citations) Rabbit anti-synapsin-1 (4 citations) Anti-phospho(S553)-synapsin 1 (2 citations) Mouse anti-Synapsin-1 (2 citations) Anti-synapsin (2 citations)

3 protocols using synapsin

Immunostaining of Neural Cell Cultures

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Cells (~ 1 × 10⁵) were cultured on a cover glass in a 12‐well plate with 700 μL of medium. The neural cells were allowed to grow to desired morphology and density before staining procedure. Cells were first washed once with PBS and fixed by 4% paraformaldehyde/4% sucrose in PBS at room temp, followed by permeabilization and DNA denaturation by 0.2% TritonX‐100 in 4 m HCl. After that, the cells were washed with PBS and blocked in 80 μL BSA (3%). The cells were incubated with anti‐FOXG1 (ab18259; Abcam), Ki‐67 (BD, 550609), Otx1/2 (ab21990; Abcam, Cambridge, MA, USA), PAX6 (ab195045; Abcam), NESTIN (BD, 561230), Nkx2.1 (MAB5460; Millipore, Darmstadt, Germany), MAP2 (M4403; Sigma, St. Louis, MO, USA), GABA (A2052; Sigma) VGAT (131011; Synaptic systems, Goettingen, Germany), SYNAPSIN (Abcam), TBR1 (ab31940; Abcam), GAT1 and Glutamate (ab1511; Millipore) in BSA (3%) at 4 °C overnight, and then conjugated with and Hoechst 33342 or DAPI. The glass slides were mounted with a cover slip before imaging.

Tu J., Cao D., Li L., Cheung H, & Chan W. (2018). MicroRNA profiling during directed differentiation of cortical interneurons from human‐induced pluripotent stem cells. FEBS Open Bio, 8(4), 502-512.

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Immunofluorescent Staining Protocol for Neuronal Cultures

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NDC and PS-1-derived cultures were fixed at room temperature with 4% paraformaldehyde (Lamp2 samples were post-treated with cold 100% methanol for 5 minutes at −20°C) 4 days post-transduction or transfection. Fixed cultures were blocked for 45 minutes at room temperature in blocking solution (3% bovine serum albumin (BSA), 0.3% Triton X-100, 1x phosphate buffered saline (PBS)), stained for 2 hours at room temperature or overnight at 4°C, and washed twice with PBS (5 minutes/wash). The primary antibodies used were Map2b (1:500; Sigma), β -III-tubulin (1:500; Covance), synapsin (1:2000; Abcam), lamp2 (1:500; DSHB). All secondary antibodies were diluted 1:1000 in blocking solution and applied for 45 minutes at room temperature. Antifade solution (Prolong, Thermo) was applied prior to mounting the coverslips to glass slides, in an attempt to preserve the immunofluorescent signal. Samples were imaged with a Leica confocal microscope and data collection and analysis was performed using ImageJ.

Reilly P., Winston C.N., Baron K., Trejo M., Rockenstein E.M., Akers J.C., Kfoury N., Diamond M., Masliah E., Rissman R.A, & Yuan S.H. (2017). Novel human neuronal tau model exhibiting neurofibrillary tangles and transcellular propagation. Neurobiology of disease, 106, 222-234.

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Hippocampal Protein Extraction and Analysis

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Each gram of hippocampi were homogenized with 30 µl cocktail protease inhibitor (Roche Molecular Biochemicals, Germany) and 3 ml RIPA buffer (US Biological, USA). After centrifugation at 12000 rpm (4 •C), the supernatant was collected and stored at -20 •C. BCA assay and spectrophotometry were used to assess the total protein concentration. The supernatant sample (50µg of protein) was separated by 10% SDS-PAGE and transferred to PVDF membrane (Millipore Inc., Darmstadt, Germany). After blocking with 5% skim milk for 2 hours, the membrane was incubated with primary antibody overnight: Rac1 (1:500, sc-514583, Santa Cruz Biotechnology), Tiam1 (1:500, sc-393315, Santa Cruz Biotechnology), α-chimaerin (1:500, sc-365985, Santa Cruz Biotechnology), Bcr (1:500, sc-104, Santa Cruz Biotechnology), GluR-1 (1:500, sc-13152, Santa Cruz Biotechnology), Synapsin 1(1:500, ab254349, Abcam), and PSD-95 (1:500, sc-32290, Santa Cruz Biotechnology). The speci city of these primary antibodies has been veri ed (Mao et Tsai et al. 2012 (link)). The membrane was incubated with a mixture of anti-rabbit IgG (Golden Bridge, Zhongshan, China) and horseradish peroxidase (HRP) for 1 hour at room temperature. Quantity One software (Bio-Rad, USA) was used for quantitative analysis.

Zhu X., Zhang F., You Y., Wang H., Yuan S., Wu B., Zhu R., Liu D., Yan F, & Wang Z. (2023). S-Ketamine Exerts Antidepressant Effects by Regulating Rac1 GTPase Mediated Synaptic Plasticity in the Hippocampus of Stressed Rats. Cellular and molecular neurobiology, 43(1).

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