Nc 100 ccd deep cooled camera

Bioluminescence imaging (BLI) and fluorescence reflectance imaging (FRI) were performed using a NightOWL II LB 983 system equipped with a NC 100 CCD deep-cooled camera (Berthold Technologies, Bad Wildbad, Germany). For BLI, mice were injected intra-peritoneally with D-luciferin (2.9 mg in 100 μL sterile PBS, Promega), sedated at 5 min post injection and imaged at 8 min. Bioluminescence images (2 min integration period, 4 × 4 binning) and photo (100 ms exposition) were taken in prone and supine positions, respectively. A low light emitting standard (Glowell, LUX biotechnology, UK) was placed next to the animal during each image acquisition as a quality control. For FRI, images (1 s exposition, 1 × 1 binning) and photographs (100 ms exposition) were acquired. Excitation was performed at 590/20 nm and fluorescence emission was detected at 680/30 nm. Quantification was done by placing a ROI manually on the tumor and measuring the mean light intensity (in photons.s⁻¹.cm⁻²) within the ROI using Indigo software (Berthold Technologies). Pseudo-color images were generated using ImageJ software.

BLI was performed at the Vivoptic platform (Bordeaux University & CNRS, UMS 3767) using a NightOWL II LB 983 calibrated system equipped with an NC 100 CCD deep-cooled camera (Berthold Technologies™, Bad Wildbad, Germany). Mice were injected intraperitoneally with d-luciferin (2.9 mg in 100 µL PBS, Promega™, Madison, WI, USA,) and sedated 5 min later. Bioluminescence images (1 min exposure, 4 × 4 binning) and photographs (100 ms exposure) were taken 8 min after the luciferin injection. A low light emitting standard (Glowell, Lux Biotechnology Limited, Edinburgh, UK) was placed next to the animal during each image acquisition to provide a quality control. Pseudocolor images representing the spatial distribution of emitted photons were generated using IndiGO 2 software (Berthold Technologies™) and superposed to the corresponding photographs.