HPLC grade water, acetonitrile (ACN), and methanol (MeOH) were purchased from Thomas Scientific (Swedesboro, NJ, USA) and Sigma-Aldrich (St. Louis, MO, USA). Water from a Milli-Q® water purification system (MilliporeSigma, Burlington, MA, USA) was also used. HPLC grade methyl tert-butyl ether (MTBE) was purchased from VWR (Radner, PA, USA). Sodium acetate buffer (pH 4.65, 0.2 M) was obtained from Fluka (Honeywell Products, Charlotte, NC, USA). β-Glucuronidase from Helix pomatia (type HP-2, >100000 units/mL) was purchased from Sigma-Aldrich. ZEN, ZER, α-ZOL, β-ZOL, β-ZAL, ZAN standards were all purchased from Sigma-Aldrich. Internal standards Rac-Zearalenone-d6 (ZEN-d6; used for ZAN and ZEN) and α-Zearalenol-d7 (α-ZOL-d7; used for the other compounds) were obtained from Toronto Research Chemicals (North York, ON, Canada). Chem Elut supported liquid extraction (SLE) columns (3 mL, unbuffered) were from Agilent (Santa Clara, CA, USA); Discovery DSC-NH2 solid-phase extraction tubes (1 mL/100 mg) were from Supelco (Bellefonte, PA, USA); Sep-Pak silica cartridges (3 cc/500 mg) were from Waters (Milford, MA, USA).
β glucuronidase from helix pomatia type hp 2
Manufactured by Merck Group
Sourced in United States
β -glucuronidase from Helix pomatia type HP-2 is an enzyme found in the digestive tract of the Roman snail (Helix pomatia). It catalyzes the hydrolysis of β-glucuronides, which are conjugates of glucuronic acid and various compounds. The enzyme is commonly used in analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Milli q water purification system, by Merck Group (3 mentions)
Hplc grade methyl tert butyl ether, by Avantor (2 mentions)
Rac zearalenone d6, by LGC (2 mentions)
α zearalenol d7, by LGC (2 mentions)
7 ethoxycoumarin, by Merck Group (2 mentions)
Trans stilbene, by Merck Group (2 mentions)
β glucuronidase from escherichia coli type 9 a, by Merck Group (2 mentions)
Ethyl acetate, by Thermo Fisher Scientific (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
α zol, by Merck Group (1 mentions)
β zol, by Merck Group (1 mentions)
β zal, by Merck Group (1 mentions)
Sep pak silica cartridge, by Waters Corporation (1 mentions)
Halothane, by Merck Group (1 mentions)
Pentane, by Thermo Fisher Scientific (1 mentions)
Isooctane, by Thermo Fisher Scientific (1 mentions)
2 naphthol 2 nap, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
11 protocols using β glucuronidase from helix pomatia type hp 2
1
Sensitive Zearalenone Metabolites Analysis
2
Coumarin Glucuronidation Kinetics Assay
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Trans-stilbene, 7 - ethoxycoumarin, β - glucuronidase from Escherichia coli type IX-A, β -glucuronidase from Helix pomatia type HP-2, and halothane were purchased from Sigma Aldrich (USA). Racemic liquiritigenin was purchased from Extrasynthese (France). HPLC grade acetonitrile and water were purchased from J. T. Baker (USA). Phosphoric acid was purchased from Aldrich Chemical Co. Inc. (USA). Silastic® laboratory tubing was purchased from Dow Corning Corporation (USA). Intramedic® polyethylene tubing was purchased from Becton Dickinson Primary Care Diagnostics, Becton Dickinson and Company (USA). Monoject® 23 gauge (0.6 mm × 25 mm) polypropylene hub hypodermic needles were purchased from Sherwood Medical (USA). Synthetic absorbable surgical sutures were purchased from Wilburn Medical US (USA). Rats were obtained from Simonsen Labs (USA). Ethics approval for animal experiments was obtained from University of Manitoba.
3
Quantification of OH-PAH Metabolites
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OH-PAH standards including 1-hydroxypyrene [1PYR (MW: 218.26 g/mol, purity 98%)] and 2-naphthol [2NAP, (MW: 144.17 g/mol, purity 99%)] were purchased from Sigma-Aldrich Inc., (St Louis, MO). HPLC-grade reagents including acetonitrile, methanol, pentane, isooctane and ethyl acetate were obtained from Fisher Scientific (Pittsburgh, PA). Sodium acetate (reagent grade, >99%), potassium dihydrogen phosphate (ACS reagent, ≥99%) and the enzyme β-glucuronidase (from Helix pomatia, type HP-2) was purchased from Sigma-Aldrich. Type I ultrapure water was obtained from Milli-Q water purification system (Millipore, Bedford, MA). All stock solutions were prepared by dissolving 1 mg of the native metabolites in 100 mL of methanol to obtain a 10 µg/mL concentration. Two working solutions of 2NAP and 1PYR were prepared by diluting the stock solution in methanol to yield a final concentration of either 0.5 µg/mL or 0.05 µg/mL. Calibration solutions (6 levels) were prepared in blank pooled urine matrix by spiking appropriate volumes of the working solutions to yield the following concentration ranges: 2NAP, 1–25 ng/mL and 1PYR, 0.1–6 ng/mL. All stock and working standards were prepared in amber vials and stored at −20 °C until further use.
4
Quantification of Zearalenone and Metabolites
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Reference standards of Zen (≥ 99%), Zer (≥ 98%, HPLC grade), zearalanone (Zan; ≥ 98%), β-zearalanol (bZal; ≥ 96%, HPLC grade), α-zearalenol (aZol; ≥ 98%), and β-zearalenol (bZol; ≥ 97%) were obtained from Sigma-Aldrich. The internal standards Rac-Zearalenone-d6 (Zen-d6; ≥ 96.9%) and α-zearalenol-d7 (aZol-d7; ≥ 98%) were obtained from Toronto Research Chemicals. Sodium acetate buffer solution (pH = 4.65, 0.2 M) was purchased from Honeywell Fluka. The enzyme β-glucuronidase from Helix pomatia (type HP-2, > 100,000 units/mL, sulfatase activity < 7500 units/mL) was obtained from Sigma-Aldrich. HPLC-grade methyl tert-butyl ether (MTBE) and methanol (MeOH) were purchased from VWR. HPLC-grade acetonitrile (ACN) was purchased from Honeywell. Water was obtained from a Milli-Q water purification system (Millipore).
5
Analysis of Paracetamol and Orthocetamol
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Ammonium acetate, potassium dihydrogen orthophosphate, β-glucuronidase from Helix pomatia (Type HP-2 at 100,000 units/ml) and pancreatin (8xUSP) were obtained from Sigma-Aldrich Company Ltd. (Dorset, UK). Acetonitrile, ethyl acetate, hexane and methanol were obtained from Fisher Scientific UK Ltd. (Loughborough, UK).
Optima grade formic acid was obtained from LGC (Middlesex, UK). Sodium hydroxide solution at 40% (v/v) and HiPerSolv water were obtained from VWR International Ltd.
(Lutterworth, UK). Water was purified using a Triple Red Duo Water system (Triple Red Laboratory Technology).
Paracetamol was purchased as powder from Sigma-Aldrich, whilst orthocetamol was purchased as powder from Toronto Research Chemicals (Canada). Deuterium labelled Paracetamol-d4 was purchased as powder from Cayman Chemicals (Cambridge, UK). Stock solutions were prepared at 2 mg/ml (Paracetamol and orthocetamol) and 1 mg/ml (Paracetamol-d4) in methanol and stored at -20ºC. Stock solutions of Paracetamol and orthocetamol were mixed 50:50 (v/v) to obtain a mixed standard at 1 mg/ml, which was subsequently diluted with methanol to obtain spiking solutions at appropriate concentrations.
Optima grade formic acid was obtained from LGC (Middlesex, UK). Sodium hydroxide solution at 40% (v/v) and HiPerSolv water were obtained from VWR International Ltd.
(Lutterworth, UK). Water was purified using a Triple Red Duo Water system (Triple Red Laboratory Technology).
Paracetamol was purchased as powder from Sigma-Aldrich, whilst orthocetamol was purchased as powder from Toronto Research Chemicals (Canada). Deuterium labelled Paracetamol-d4 was purchased as powder from Cayman Chemicals (Cambridge, UK). Stock solutions were prepared at 2 mg/ml (Paracetamol and orthocetamol) and 1 mg/ml (Paracetamol-d4) in methanol and stored at -20ºC. Stock solutions of Paracetamol and orthocetamol were mixed 50:50 (v/v) to obtain a mixed standard at 1 mg/ml, which was subsequently diluted with methanol to obtain spiking solutions at appropriate concentrations.
6
Pharmacokinetics of Pinocembrin and Pinostrobin
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Trans-stilbene, 7-ethoxycoumarin, β-glucuronidase from Escherichia coli type IX-A, β -glucuronidase from Helix pomatia type HP-2, and halothane were purchased from Sigma (St. Louis, MO, USA). Racemic pinocembrin (50:50, S:R) was purchased from Extrasynthese (France). Racemic pinostrobin (50:50, S:R) was purchased from Indofine Chemical Company (Hillsborough, NJ, USA). HPLC grade acetonitrile and water were purchased from J. T. Baker (Phillipsburg, NJ, USA). Phosphoric acid was purchased from Aldrich Chemical Co. Inc. (Milwaukee, WI, USA). Silastic laboratory tubing was purchased from Dow Corning Corporation, (Midland, MI, USA). Intramedic polyethylene tubing was purchased from Becton Dickinson Primary Care Diagnostics, Becton Dickinson and Company (Sparks, MD, USA). Monoject 23 gauge (0.6 mm 25 mm) polypropylene hub hypodermic needles were purchased from Sherwood Medical (St. Louis, MO, USA). Synthetic absorbable surgical sutures were purchased from Wilburn Medical US (Kernesville, NC, USA). Rats were obtained from Simonsen Labs (Gilroy, CA, USA). Ethics approval for animal experiments was obtained from University of Manitoba.
7
Celecoxib and Zaleplon Assay Protocol
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Celecoxib and zaleplon were kind donations by Ipca Laboratories Limited, Indore, India, both having an assay purity of ≈ 99.8%. β-glucuronidase from Helix pomatia (type HP2) and NADPH were purchased from Sigma Chemical Co. Ltd.. Hydroxymethyl celecoxib and celecoxib carboxylic acid were obtained from Alsachim (France). All organic solvents were either HPLC grade or LC/MS grade and purchased from Fisher Scientific.
Horse liver microsomes were bought from Xeno Tech U.S.A for in vitro studies.
Horse liver microsomes were bought from Xeno Tech U.S.A for in vitro studies.
8
Quantitative Bioanalysis of Metoprolol Metabolites
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Metoprolol, α-OH-metoprolol, O-demethylmetoprolol, metoprolol phenylacetate, and metoprolol-d7 were purchased from TRC (Toronto, Canada). 1′-OH-Midazolam and Midazolam-d6 were acquired from Lipomed (Arlesheim, Switzerland), while rifampicin, ketoconazole, quinidine, and β-glucuronidase (type HP-2 from Helix pomatia) were obtained from Sigma-Aldrich (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland). Midazolam was provided by Roche (Hoffmann-La Roche, Basel, Switzerland). Formic acid, HPLC grade methanol, and HPLC grade water were purchased from Merck (Darmstadt, Germany). The media used were purchased from GIBCO (Lucerne, Switzerland).
Stock solutions, calibration spiking solutions, and quality controls were prepared in DMSO. Calibration standards and quality controls were prepared by enriching the respective medium with the corresponding spiking solutions. Internal standard solutions containing the deuterated cocktail probe drugs were prepared in methanol.
Stock solutions, calibration spiking solutions, and quality controls were prepared in DMSO. Calibration standards and quality controls were prepared by enriching the respective medium with the corresponding spiking solutions. Internal standard solutions containing the deuterated cocktail probe drugs were prepared in methanol.
9
Analysis of Chloramphenicol in Samples
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Acetonitrile was from Merck (Germany). Chloramphenicol–D5 (CAP-D5) and β-glucuronidase type HP-2 from Helix pomatia were obtained from Sigma-Aldrich (USA). Acetic acid, ammonium acetate, methanol, isopropanol, ethyl acetate, and octadecyl C18 cartridges (500 mg, 6 mL) were from J.T. Baker (the Netherlands). Sodium chloride (NaCl) was purchased from P.O.Ch. (Poland). Kinetex C8 column (75 mm × 2.1 mm × 2.6 μm) and C8 precolumn (4 mm × 2 mm × 4 μm) were purchased from Phenomenex (USA). All reagents were of analytical grade or higher.
Individual stock standard solution of CAP and internal standard (CAP-D5) solutions (1 mg mL-1) were prepared in methanol and stored in the dark at <–18°C, no longer than one year. The working standard and internal standard solutions at the level of 0.01 μg mL-1 were prepared in methanol and stored in the dark at <6°C, no longer than six months.
Individual stock standard solution of CAP and internal standard (CAP-D5) solutions (1 mg mL-1) were prepared in methanol and stored in the dark at <–18°C, no longer than one year. The working standard and internal standard solutions at the level of 0.01 μg mL-1 were prepared in methanol and stored in the dark at <6°C, no longer than six months.
10
Synthesis and Characterization of Thujone Metabolites
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α-Thujone, the hydroxythujones 2-OH, 4-OH and 7-OH and their isotopically labeled compounds were prepared according to Thamm et al. [4 (link)]. Acetonitril (LC-MS grade) and water (LC-MS grade) were purchased from VWR (Radnor, PA, USA). β-Glucuronidase (type HP-2 from Helix pomatia) and hydrochloric acid (conc.) were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium chloride was purchased from Merck KGaA (Darmstadt, Germany).
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