On day 28 after establishment of the subcutaneous tumor model, splenocytes or blood was isolated from each group. After lysing red blood cells, the remaining cells were stained with FITC-labeled anti-mCD11c (catalog number: 11-0114), PE-labeled anti-mCD80(catalog number: 12-0801), FITC-labeled anti-mCD4 (catalog number: 11-0042), or APC-labeled anti-mCD8 (catalog number: 17-0081) (all from eBioscience) for 30 minutes at room temperature. The positive cells were measured by flow cytometry.
Apc labeled anti mcd8
Manufactured by Thermo Fisher Scientific
The APC-labeled anti-mCD8 is a flow cytometry reagent used to identify and quantify CD8-positive cells. It consists of an anti-mouse CD8 monoclonal antibody conjugated to the Allophycocyanin (APC) fluorescent dye. The core function of this product is to serve as a specific detection tool for CD8+ cells in mouse samples during flow cytometric analysis.
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Lab products found in correlation
Fitc labeled anti mcd4, by Thermo Fisher Scientific (2 mentions)
Fitc labeled anti mcd11c, by Thermo Fisher Scientific (1 mentions)
Pe labeled anti mcd80, by Thermo Fisher Scientific (1 mentions)
Pe labeled anti ifn γ, by BioLegend (1 mentions)
Pe labeled anti foxp3 antibody, by BioLegend (1 mentions)
Pe labeled anti pd1, by BioLegend (1 mentions)
2 protocols using apc labeled anti mcd8
1
Tumor-Infiltrating Immune Cell Analysis
2
T-cell Subset Analysis of Immunotherapy
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To determine the therapeutic effect of the SA‐IL‐2‐modified vaccine together with PD‐1 blockade on T‐cell subsets, 100 μL blood was collected from each group on day 19 after tumor injection. To detect CD4+ and CD8+ T‐cell subsets, cells were stained with FITC‐labeled anti‐mCD4 and APC‐labeled anti‐mCD8 (eBioscience). To detect IFN‐γ+CD8+, PD‐1+CD8+ and CD4+Foxp3+ T‐cell subsets, cells were first stained with APC‐labeled anti‐mCD8 or FITC‐labeled anti‐mCD4 (Biolegend, San Diego, CA, USA), and then stained with PE‐labeled anti‐IFN‐γ, PE‐labeled anti‐PD‐1 or PE‐labeled anti‐FoxP3 antibody (Biolegend). T‐cell subsets were detected by flow cytometry.
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