Log In Sign up
The largest database of trusted experimental protocols

Bactiter glo reagent

Manufactured by Promega
Visit Supplier
Sourced in United States

The BacTiter-Glo reagent is a luminescence-based assay that measures the presence of viable bacterial cells in a sample. It provides a quantitative assessment of bacterial viability without requiring cell lysis or extensive sample preparation.

Automatically generated - may contain errors

Lab products found in correlation

Infinite m200, by Tecan (3 mentions) Glomax 20 20 luminometer, by Promega (3 mentions) Glomax, by Promega (3 mentions) Millex gp, by Merck Group (2 mentions) Polarstar omega, by BMG Labtech (2 mentions) Infinite 200 pro, by Tecan (2 mentions) Bactiter glo microbial cell viability assay, by Promega (2 mentions) Synergy ht multiple detection microplate reader, by Agilent Technologies (2 mentions) Bactiter glo microbial cell viability assay kit, by Promega (2 mentions) Infinite m200 pro, by Tecan (1 mentions) Flexstation 3, by Molecular Devices (1 mentions) Atp standard, by Promega (1 mentions) Spectramax i3x reader, by Molecular Devices (1 mentions) Synergy 2 multi mode reader, by Agilent Technologies (1 mentions) Mitoprobe jc 1 assay kit, by Thermo Fisher Scientific (1 mentions) Atplite 1step assay kit, by PerkinElmer (1 mentions) Atp sulfurylase, by New England Biolabs (1 mentions) Synergy ht multi detection microplate reader, by Agilent Technologies (1 mentions) Synergy 4, by Agilent Technologies (1 mentions) Synergy htx multi mode microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

BacTiter-Glo (47 citations) BacTiter-GloTM (14 citations) BacTiter-Glo™ Microbial Cell Viability Assay Reagent (11 citations) BacTiter-GloTM reagent (7 citations) BacTiter-GloTM Microbial Cell Viability Assay reagent (2 citations) BacTiter-Glo lysis reagent (2 citations)

54 protocols using bactiter glo reagent

1

Mitochondrial Potential and ATP Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hyphae were incubated 2 hours with atovaquone, mitochondria inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP) or DMSO in RPMI 1640. RPMI was then removed and fresh RPMI 1640 (no phenol red) containing JC1 (2 μM) probe was added for 15 minutes, and fluorescence was quantified per the manufacturer's directions (MitoProbe JC1 Assay Kit; ThermoFisher Scientific, Waltham, MA, USA). JC-1 dye accumulates in healthy mitochondria (intact mitochondrial potential), indicated by a fluorescence emission shift from green (∼529 nm) to red (∼590 nm); consequently, mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio.
For ATP measurements, hyphae were treated with atovaquone or medium alone in 200 μL RPMI 1640 for 2 hours; 175 μL media was removed and 25 μL BacTiter-Glo Reagent (Promega, Madison, WI, USA) was added to the remaining 25 μL culture media and incubated for 10 minutes at RT. BacTiter-Glo Reagent generates a luminescent signal proportional to the amount of ATP in a sample using a proprietary luciferase reagent (Promega). Luminescence was measured using the Cytation5 reader.
Clark H.L., Minns M.S., Sun Y., de Jesus T., Ghannoum M.G, & Pearlman E. (2018). Atovaquone Impairs Growth of Aspergillus and Fusarium Keratitis Isolates by Modulating Mitochondrial Function and Zinc Homeostasis. Investigative Ophthalmology & Visual Science, 59(3), 1589-1598.
+ Open protocol
+ Expand
2

Mycobacterium tuberculosis Viability and ADP/ATP Ratio

Check if the same lab product or an alternative is used in the 5 most similar protocols
Log-phase Mtb cultures were exposed to drugs for 24 h and mixed with BacTiter-Glo reagent (v/v 4:1) (Promega) followed by incubation in the dark for 10 min. Luminescence (relative light units, RLUs) was read in a TECAN Infinite M200. For ADP detection and calculation of the ADP/ATP ratio, we exposed log-phase Mtb (OD600 of 0.01) to LPZS for 24 h in a white 96-well plate. BacTiter-Glo reagent (v/v 4:1) was added and luminescence was determined after 2 min (RLUA). Plates were incubated in the dark for 15 min and read again, which provided background ATP signals prior to ADP measurements (RLUB). Five microlitre of an ADP-converting enzyme (Sigma Alrich, MAK135E) were added, followed by a third measurement after 1 min of incubation (RLUC). The ADP/ATP ratio was calculated using the following formula: ADP/ATP=(RLUC−RLUB)/RLUA.
Rybniker J., Vocat A., Sala C., Busso P., Pojer F., Benjak A, & Cole S.T. (2015). Lansoprazole is an antituberculous prodrug targeting cytochrome bc1. Nature Communications, 6, 7659.
+ Open protocol
+ Expand
3

Cellular ATP Measurement by Bioluminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular ATP levels were determined in the three tested strains at different OD values by a luciferase bioluminescence assay. Briefly, 100 µl of each sample was mixed with an equal volume of BacTiter-Glo reagent (Promega) in a 96-well plate, incubated for 5 min, and then the luminescence was measured using the previously described luminometer.
Enany S., Yoshida Y., Tateishi Y., Ozeki Y., Nishiyama A., Savitskaya A., Yamaguchi T., Ohara Y., Yamamoto T., Ato M, & Matsumoto S. (2017). Mycobacterial DNA-binding protein 1 is critical for long term survival of Mycobacterium smegmatis and simultaneously coordinates cellular functions. Scientific Reports, 7, 6810.
+ Open protocol
+ Expand
4

Quantifying Viable Candida albicans in Planktonic Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the relative amount of live C. albicans in the media of the planktonic-based activity assay, 100 µL of the homogeneous fungal suspension from each well were transferred to white 96-well flat-bottomed microplates, to which 100 µL of the BacTiterGlo reagent (Promega Corporation, Madison, WI, USA) was added [21 (link)]. After 20 min of shaking at room temperature, the luminescence was measured in an Infinite M200PRO plate reader (Tecan, Männedorf, Switzerland). The percentage of viable fungi in the SRV-CTZ group in comparison to SRV-placebo group was calculated using the following formula: (LumCTZ/LumPL) × 100, where LumCTZ is the average luminescence of the samples from the SRV-CTZ group and LumPL the average luminescence of the samples from the SRV-placebo group.
Sionov R.V., Gati I., Kirmayer D., Friedman M., Steinberg D, & Gross M. (2021). Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against Candida albicans: An In Vitro Study. Molecules, 26(17), 5395.
+ Open protocol
+ Expand
5

Bioluminescent Yeast Cell Viability Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All strains were cultured overnight in YPD at 30°C. Cells were then subcultured in fresh medium until the log phase of growth was reached. Next, 2 × 106 cells of each strain were mixed completely with equal volumes of the BacTiter-Glo reagent (Promega Corporation, Madison, WI) (Lin et al., 2019 (link); Li et al., 2021 (link)), followed by incubation at room temperature for 15 min in the dark. Finally, luminescent signals were detected using the full-wavelength multifunctional enzyme-mark instrument (SpectraMax Paradigm).
Zeng L., Huang Y., Tan J., Peng J., Hu N., Liu Q., Cao Y., Zhang Y., Chen J, & Huang X. (2023). QCR7 affects the virulence of Candida albicans and the uptake of multiple carbon sources present in different host niches. Frontiers in Cellular and Infection Microbiology, 13, 1136698.
+ Open protocol
+ Expand
6

ATP Efflux Assay for Transfected HEK293 Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The ability of the transfected HEK293 cells to release ATP into the culture medium was determined using confluent monolayers as described previously [14 (link),19 (link)]. In brief, the medium was removed and replaced with 50 μL efflux buffer, consisting of 11.5 mM HEPES (pH 7.4), 130 mM NaCl, 5 mM MgCl2, 1.5 mM CaCl2, and 11.5 mM glucose. The cells were then incubated for 1 hr at 27 °C. Next, 50 µL BactiterGlo reagent (Promega) dissolved in efflux buffer was added to each well. Bioluminescence was subsequently determined in real time in a Flex Station 3 microplate reader (Molecular Devices, San Jose, CA, USA) as detailed previously [14 (link),19 (link)]. The real-time ATP efflux assay was run at 27 °C for the first 1 h and then at 37 °C for 2 hr. The initial low temperature allowed endogenous ecto-nucleotidases to degrade the Abcc6-independent background ATP efflux induced by the medium change.
Szeri F., Corradi V., Niaziorimi F., Donnelly S., Conseil G., Cole S.P., Tieleman D.P, & van de Wetering K. (2021). Mutagenic Analysis of the Putative ABCC6 Substrate-Binding Cavity Using a New Homology Model. International Journal of Molecular Sciences, 22(13), 6910.
+ Open protocol
+ Expand
7

Antimicrobial Screening of S. aureus

Check if the same lab product or an alternative is used in the 5 most similar protocols
S. aureus ATCC 25923 strain was grown in Mueller Hinton II broth at 35°C overnight. The overnight culture was diluted in fresh broth and used as inoculum for the antimicrobial screen. Working stocks (50X) of test compounds were prepared in DMSO. Each well of the 384-well plate contained 30 μl of the inoculum and 0.6 μl of the 50X compound working stock. Plates were incubated at 35°C for 18 hours and 30 μl of the BacTiter-Glo™ reagent (Promega) was added. Relative luminescent units (RLU) were measured using and normalized to growth control wells (2% DMSO) and expressed as % Growth. IC50 values were calculated using the Levenberg–Marquardt nonlinear regression algorithm and defined as the compound concentration that produced 50% of the growth inhibitory response. The IC90 value was defined as the compound concentration that produced 90% of the growth inhibitory response [9 ,10 ].
Chandrasekera N.S., Alling T., Bailey M., Korkegian A., Ahn J., Ovechkina Y., Odingo J, & Parish T. (2015). The 4-aminopiperidine series has limited anti-tubercular and anti-staphylococcus aureus activity. Journal of Negative Results in Biomedicine, 14, 4.
+ Open protocol
+ Expand
8

ATP Quantification in Biological Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total ATP (ATPt) and extracellular ATP (ATPe) were determined using the BacTiter-Glo™ reagent (Promega Corporation) and a Promega Glomax 20/20 luminometer as described elsewhere89 (link)–91 (link). Briefly, samples (500 μL) and ATP reagent (50 μL) were warmed to 38°C simultaneously in separate sterile Eppendorf tubes then combined, incubated at 38 °C for an additional 20 s, and measured. Luminescence data were collected as relative light units (RLU) and converted to ATP (nM) by means of a calibration curve made with a known ATP standard (10 uM; Promega) diluted in ATP-free, sterile water (autoclaved and 0.1 μm filtered dechlorinated tap water). For extracellular ATP analysis, each sample was filtered through a 0.1 μm sterile syringe filter (Millex®-GP, Millipore) followed by analysis as described above. The intracellular and cell-bound ATP (ATPi) was calculated by subtracting the extracellular ATP from the total ATP for each individual sample. ATP was measured in triplicate for all samples.
Healy H.G., Ehde A., Bartholow A., Kantor R.S, & Nelson K.L. (2024). Responses of drinking water bulk and biofilm microbiota to elevated water age in bench-scale simulated distribution systems. NPJ Biofilms and Microbiomes, 10, 7.
+ Open protocol
+ Expand
9

MTB Antibiotic Susceptibility Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Twofold dilutions of antitubercular agents were prepared in Middlebrook 7H9 medium in a volume of 20 μL in 96-well culture plates. Approximately 180 μL of 106 CFU/mL MTB strains was added, yielding a final testing volume of 200 μL. The plates were incubated at 37°C; on the seventh day, 100 μL of culture was taken from each well and mixed with an equal volume of Bac Titer-Glo® reagent (Promega, Madison, WI, USA). The assay was performed according to the manufacturer's instructions. Luminescence was measured on a POLARstar Omega (BMG Lab Tech, Cary, NC) microplate reader at an integration time of 500 milliseconds.
Peterson E.J., Ma S., Sherman D.R, & Baliga N.S. (2016). Network analysis identifies Rv0324 and Rv0880 as regulators of bedaquiline tolerance in Mycobacterium tuberculosis. Nature microbiology, 1(8), 16078.
+ Open protocol
+ Expand
10

High-Throughput Screening of Antibacterial Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
High-throughput screening was performed at the University of Michigan Center for Chemical Genomics. Wild-type E. faecalis OG1RF was grown overnight in MHB + 10% Terrific Broth with a final glycerol concentration of 4%. Stationary-phase cultures were diluted into fresh media containing 8 µg/ml CTRX and incubated overnight at 37°C in 384-well plates containing approximately 10 µM final concentration of stock compound. Using the BacTiter-Glo reagent (Promega) per manufacturer’s instructions, total ATP was measured as a surrogate for cell-density. Confirmation and dose-response screening were performed similarly but included wells lacking CTRX.
Hoff J.S, & Kristich C.J. (2016). Thymidylate limitation potentiates cephalosporin activity towards enterococci via an exopolysaccharide-based mechanism. ACS chemical biology, 11(6), 1561-1568.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!