Zb5 msi fused silica capillary column

The qualification of the sample extract was performed using a GC-MS/MS system (GCMS-TQ8040; Shimadzu, Kyoto, Japan) equipped with a ZB5-MSi fused-silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Phenomenex, Torrance, CA, USA). Grade 5.0 helium was used as the carrier gas. Column flow was 1 mL/min. The injection of a 1 μL sample was performed using an AOC-20i + s type autosampler (Shimadzu, Kyoto, Japan). The injector was working at a temperature of 310 °C. The following temperature program was applied: the oven temperature was held at 60 °C for 2 min and was subsequently increased linearly at a rate of 6 °C/min to 310 °C, where it was held for 15 min. The mass spectrometer was operated in EI mode at 70 eV; the ion source temperature was 225 °C. The mass spectra were measured in the range of 40–450 amu. The amounts of the individual analytes were estimated with the peak normalization method.

For qualification and quantification of the QuEChERS extracts, a gas chromatograph with a tandem mass spectrometer detector (GCMS-TQ8040; Shimadzu, Kyoto, Japan) equipped with a Zebron ZB5-MSi fused-silica capillary column (30 m × 0.25 mm i.d., 0.25 µm film thickness; Phenomenex) was used. Helium (grade 5.0) was used as the carrier gas, and argon (grade 5.0) was used as the collision gas. Column flow was 1.56 mL/min, and 1 µL of the sample was injected using an AOC-20i/s autosampler (Shimadzu). The injector was set to high-pressure mode (250.0 kPa for 1.5 min; column flow at initial temperature was 4.90 mL/min) at 320 °C. The following temperature program was applied:
The oven temperature was held at 60 °C for 2 min and was subsequently increased linearly at a rate of 10 °C/min to 320 °C, where it was held for 15 min.
The mass spectrometer was operated in multiple reaction monitoring (MRM) mode using electron ionization (EI) at 70 eV and an ion source temperature of 220 °C.
Blank whole blood samples were analyzed to confirm the absence of interference peaks at the retention times of the TMS derivatives of THC, 11-OH-THC, 11-COOH-THC and the internal standards of THC-d₃, 11-OH-THC-d₃ and 11-COOH-THC-d₃.

The qualification of the sample extract was performed using a GC-MS/MS system (GCMS-TQ8040; Shimadzu, Kyoto, Japan) equipped with a ZB5-MSi fused-silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness; Phenomenex, Torrance, CA, USA). Grade 5.0 helium was used as the carrier gas. Column flow was 1 mL/min. The injection of a 1 μL sample was performed using an AOC-20i + s type autosampler (Shimadzu, Kyoto, Japan). The injector was working at a temperature of 310 °C. The following temperature program was applied: the oven temperature was held at 60 °C for 2 min and was subsequently increased linearly at a rate of 6 °C/min to 310 °C, where it was held for 15 min. The mass spectrometer was operated in EI mode at 70 eV; the ion source temperature was 225 °C. The mass spectra were measured in the range 40–450 amu. The amounts of the individual analytes were estimated by the peak normalization method.