Rat il 1β elisa kit

Concanavalin A (con A) was purchased from Alfa Aesar, Erlenbachweg 2, Kandel, (Germany). Naloxone hydrochloride was purchased from abcam Biotechnology, Cambridge, (UK). Alanine amino transferase (ALT), aspartate amino transferase (AST), albumin, and bilirubin were purchased from Bio-Med diagnostics, (USA). Reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and thiobarbituritic acid reactive substance (TBARS) assay kits were purchased from Bio-diagnostic, (Egypt). Rat interferon-γ (IFN-γ) enzyme-linked immunosorbent assay (ELISA) kit, rat TNF-α ELISA kit, rat IL-6 ELISA kit and rat IL-1β ELISA kit were purchased from MyBioSource, San Diego, California, (USA), Cusabio, Houston, (USA), R&D Systems, McKinley Place NE, Minneapolis, (USA) and MyBioSource, San Diego, California, (USA), respectively. An antibody raised against Nuclear factor kappa B (NF-κB p65), c-Jun N-terminal kinase (JNK), Nuclear factor erythroid 2 (Nrf2), Heme oxygenase-1 (HO-1), TLR4 and β-actin were purchased from Thermo Fisher Scientific, Waltham, (USA). Goat Anti-Rabbit HRP linked IgG and Goat Anti-Mouse HRP linked IgG were obtained from abcam Biotechnology, Cambridge, (UK).