Lps from p aeruginosa serotype 10

Manufactured by Merck Group

Sourced in United States, Italy

LPS from P. aeruginosa serotype 10 is a purified lipopolysaccharide product derived from Pseudomonas aeruginosa bacteria. It serves as a standard or reference material for research and experimentation purposes.

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P. aeruginosa LPS (11 citations) P. aeruginosa (11 citations) LPS from P. aeruginosa (5 citations) P. aeruginosa LPS (serotype 10) (4 citations) LPS from P. aeruginosa 10 (2 citations)

2 protocols using lps from p aeruginosa serotype 10

Omidenin Attenuates LPS-Induced ALI in Mice

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Male C57BL/6 mice (age, 8–10 weeks; weight, 18–22 g) were purchased from Charles River
(Beijing, China) and randomly divided into 5 groups (the control group, LPS group, LPS +
12.5 mg/kg OMT group, LPS + 25 mg/kg OMT group, and LPS + 50 mg/kg OMT group) (18
mice/group). After anesthetization, the mice in the LPS group were intratracheally
administered 50 µg LPS from P. aeruginosa serotype 10
(product no.: L9143) (Sigma-Aldrich Corp., St. Louis, MO, USA) in 70 µl
PBS to establish the ALI model. The mice in the control group received the same volume of
PBS in the same way as described above. The mice in the LPS + OMT groups were
intraperitoneally injected with 12.5, 25, or 50 mg/kg OMT (Meilunbio, Dalian, China) 30
min before LPS administration. Meanwhile, the mice in the control group and the LPS group
were pretreated with an equal volume of sterile saline.

Jin B, & Jin H. (2018). Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway. Experimental Animals, 67(3), 337-347.

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Synthetic Esc(1–21) Peptide Characterization

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Synthetic Esc(1–21) and its diastereomer Esc(1–21)-1c were purchased from Biomatik (Wilmington, USA). Minimum essential medium (MEM), glutamine, heat inactivated fetal bovine serum (FBS), trypsin-EDTA and penicillin-streptomycin were from Euroclone (Milan, Italy); puromycin, hydroxyurea, Triton X-100, DAPI, Mowiol 4–88, phalloidin-fluorescein isothiocyanate, LPS from P. aeruginosa serotype 10 (purified by phenol extraction), bovine serum albumin (BSA), anti-β-actin monoclonal antibody were purchased from Sigma-Aldrich (St. Luis, MO). GM6001 inhibitor, MMP-9 Inhibitor I and BrdU cell proliferation assay kit were from Millipore Merck (Merck, Milan, Italy). Human IL-8 Standard ELISA Kit was purchased from Peprotech (Rocky Hill, NJ, USA). Mouse monoclonal anti-MMP9 (2C3): sc-21733 was purchased from Santa Cruz Biotechnology; goat anti-mouse IgG-Texas Red from Jackson Immunoresearch Laboratories, West Grove, PA. Quick-RNA MiniPrep was purchased from Zymo Research, Irvine, CA, USA. iScript cDNA synthesis kit, iQ SYBR Green Supermix, non-fat dry milk and Tween-20 were from Bio-Rad Laboratories, Hercules, CA, USA. Oligonucleotide primers were purchased from Invitrogen (Carlsbad, CA). All other chemicals were reagent grade.

Cappiello F., Ranieri D., Carnicelli V., Casciaro B., Chen H.T., Ferrera L., Di Y.P, & Mangoni M.L. (2019). Bronchial epithelium repair by Esculentin-1a-derived antimicrobial peptides: involvement of metalloproteinase-9 and interleukin-8, and evaluation of peptides’ immunogenicity. Scientific Reports, 9, 18988.

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