The purities of FTX and a series of degradation products were confirmed by HPSEC-RID. The HPSEC High performance size-exclusion chromatography-refractive system included a Metrohm 709 IC pump (Metrohm, Herisau, Switzerland), a column oven (Super Co-150, Enshine, Tainan, Taiwan) equipped with a Rheodyne injector (Cotati, PA. USA) and a 200-μL sample loop, a UV-975 detector (Jasco, Tokyo, Japan) and an OPTILAB DSP interferometric refractometer (P10 cell, 690 nm, Wyatt) with the temperature controlled at 35 °C. The purity was analyzed by using 2 TSK-Gel columns (7.5 × 300 mm), PWXL 4000 and PWXL 3000, connected in series along with the TSK-Gel PWH Guard column, and eluted with 0.3 N NaNO3 at a flow rate of 0.4 mL/min.
Uv 975 detector
Manufactured by Jasco
Sourced in Japan
The UV-975 detector is a laboratory instrument designed for the detection and analysis of ultraviolet (UV) light. It is a core component used in various analytical techniques and scientific applications. The UV-975 detector measures the intensity of UV light, providing quantitative data that can be used for a range of purposes, such as identification, characterization, and monitoring of samples. The device's primary function is to accurately detect and measure UV light, offering reliable and consistent performance in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Trapezoidal long channel, by Wyatt Technology (2 mentions)
Dawn heleos 2, by Wyatt Technology (2 mentions)
Pu 980 pump, by Jasco (2 mentions)
Optilab dsp interferometric refractometer, by Wyatt Technology (1 mentions)
Eclipse 3 separation system, by Wyatt Technology (1 mentions)
Agilent 1100 series, by Agilent Technologies (1 mentions)
Vacuum degasser, by Agilent Technologies (1 mentions)
Agilent 1100 pump, by Agilent Technologies (1 mentions)
Eclipse 3, by Wyatt Technology (1 mentions)
Optilab t rex, by Wyatt Technology (1 mentions)
Rezex roa organic acids column, by Phenomenex (1 mentions)
Pu 2086 plus pump, by Jasco (1 mentions)
7 protocols using uv 975 detector
1
HPSEC-RID Analysis of FTX Purity
2
Quantifying Remnant Lipase Interactions
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An asymmetric flow field-flow fractionation (AF4) [23 ] method was applied to quantify the remnant lipase after its interaction with different concentrations of quercetin and EGCG.
The AF4 instrument used in this study was an Eclipse 3 + Separation System (Wyatt Technology, Dernbach, Germany) coupled to a multi-angle light scattering (MALS) detector (Dawn Heleos II, Wyatt Technology) with a wavelength of 663.8 nm and a UV detector operating at 280 nm (UV-975 detector, Jasco Corp., Tokyo, Japan). Both detectors were connected to the separation system. The carrier liquid was delivered using an Agilent 1100 series isocratic pump connected to a vacuum degasser (Agilent Technologies, Waldbronn, Germany). The injection onto AF4 channel was by an auto sampler Agilent 1100 series. The AF4 channel was a trapezoidal long channel (Wyatt Technology) with tip-to-tip length of 26.0 cm and inlet and outlet widths of 2.15 and 0.6 cm, respectively and with a nominal thickness of 350 μm. The accumulation wall was formed by an ultrafiltration membrane of regenerated cellulose with a nominal cut-off of 10 kDa (Merck Millipore, Bedford, MA, USA). A bovine serum albumin (BSA) solution was used for checking that separation occurred in the AF4 system and to normalize the MALS detector.
The AF4 instrument used in this study was an Eclipse 3 + Separation System (Wyatt Technology, Dernbach, Germany) coupled to a multi-angle light scattering (MALS) detector (Dawn Heleos II, Wyatt Technology) with a wavelength of 663.8 nm and a UV detector operating at 280 nm (UV-975 detector, Jasco Corp., Tokyo, Japan). Both detectors were connected to the separation system. The carrier liquid was delivered using an Agilent 1100 series isocratic pump connected to a vacuum degasser (Agilent Technologies, Waldbronn, Germany). The injection onto AF4 channel was by an auto sampler Agilent 1100 series. The AF4 channel was a trapezoidal long channel (Wyatt Technology) with tip-to-tip length of 26.0 cm and inlet and outlet widths of 2.15 and 0.6 cm, respectively and with a nominal thickness of 350 μm. The accumulation wall was formed by an ultrafiltration membrane of regenerated cellulose with a nominal cut-off of 10 kDa (Merck Millipore, Bedford, MA, USA). A bovine serum albumin (BSA) solution was used for checking that separation occurred in the AF4 system and to normalize the MALS detector.
3
Chiral HPLC Analysis using Whelk-O1 CSP
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Analytical chromatography was performed using a Jasco (Tokyo, Japan) HPLC system with a universal Rheodyne 20 μL injector and Jasco PU 980 pump. Detection was conducted using a Jasco UV975 detector. Low temperature dynamic HPLC experiments were performed using a homemade cooling device and a cooling mixture composed of acetone and dry ice.
The CSP (R,R)-Whelk-O1 (250 × 4.6 mm L × I.D., 5 µm particle size) used to perform VT-HPLC experiments was purchased from Regis Technologies Inc. Samples for the analysis were prepared by dissolving 1 mg in 1 mL of mobile phase.
The CSP (R,R)-Whelk-O1 (250 × 4.6 mm L × I.D., 5 µm particle size) used to perform VT-HPLC experiments was purchased from Regis Technologies Inc. Samples for the analysis were prepared by dissolving 1 mg in 1 mL of mobile phase.
4
HPLC Quantification of Target Compounds
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The target compounds were quantified using an HPLC system consisting of a PU-980 pump, and an UV-975 detector (Jasco International Co. Ltd., Tokyo, Japan). Chromatographic separation was achieved on a Kromasil-C18 reversed-phase column (4.6 mm × 250 mm, 5 μm, KYA Technologies Corporation, Tokyo, Japan). The conditions for HPLC analysis were as follows: the mobile phase consisted of methanol: water (90:10, v/v), which was filtered through a 0.45 μm membrane filter, then deaerated ultrasonically before use; the flow rate was maintained at 0.8 mL/min; the injection volume was 20 μL; and the column temperature was 25 °C. The UV detection wavelength was set at 254 nm (0–13 min) then at 215 nm (13–25 min). The HPLC chromatograms of the standards and samples produced under these conditions are shown in Figure 8 .
5
Asymmetrical Flow Field-Flow Fractionation
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The AF4 system was an Eclipse 3+ (Wyatt Technology, Dernbach, Germany) connected to a UV detector operating at 330 nm (UV-975 detector, Jasco Corp., Tokyo, Japan), to a multiangle light scattering (MALS) detector with a wavelength of 663.8 nm (Dawn Heleos II, Wyatt Technology) and a differential refractive index (dRI) detector (Optilab T–rEX, Wyatt Technology) operating at 658.0 nm wavelength. An Agilent 1100 pump (Agilent Technologies, Waldbronn, Germany) coupled to a vacuum degasser was used to deliver the carrier liquid. The injection of the sample onto the channel was performed by an Agilent 1100 auto-sampler. For the analysis, a trapezoidal long channel (Wyatt Technology) with 26.0 cm length and inlet and outlet widths of 2.15 and 0.6 cm, respectively, was used. The nominal channel thickness was 350 μm. An ultrafiltration membrane of regenerated cellulose was used for the accumulation wall, with 10 kDa nominal cut-off (Merck Millipore, Bedford, MA, USA). 2 mg/mL BSA solution was used to verify the performance of the channel, to normalize the MALS detector and aligning detectors.
6
BFBL Gut Model SCFA Quantification
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The BFBL gut model samples (1 mL) were collected daily from each reactor (R1, R2, and R3) for SCFAs analysis during each period. SCFAs were quantified by a method reported by Barroso et al. [26 (link)]. In short, the filtered (0.22 μm) samples were analyzed by an HPLC system equipped with Rezex ROA-Organic Acids column (300 mm × 7.8 mm, Phenomenex, Macclesfield, UK) and UV-975 detector (Jasco, Tokyo, Japan). The mobile phase was 0.005 M sulfuric acid solution with a linear gradient of 0.6 mL/min, and elution curves were monitored at 210 nm. Calibration curves were established for SCFAs with concentrations ranging from 1 to 100 mM.
7
SEC Analysis of Polymer Molecular Weights
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Molecular weight distributions
were examined by size exclusion chromatography (SEC) measurements.
The SEC setup included a PU-2086 Plus pump (Jasco), a 728 autosampler
(Bischoff), and a UV-975 detector (Jasco) adjusted to a wavelength
of 260 nm. Phenogel columns with pore sizes of 100, 500, and 1000
Å were used for separation. Samples were dissolved in THF using
concentrations of 2–3 mg mL–1. The system
was calibrated using polystyrene standards. Spectra were processed
manually. After baseline correction, data upwards of 202 g mol–1, corresponding to NPG-MA dimers, were used to calculate
the polystyrene equivalents of number and weight average molecular
masses (Mn and Mw) as well as the polydispersity index Đ.
were examined by size exclusion chromatography (SEC) measurements.
The SEC setup included a PU-2086 Plus pump (Jasco), a 728 autosampler
(Bischoff), and a UV-975 detector (Jasco) adjusted to a wavelength
of 260 nm. Phenogel columns with pore sizes of 100, 500, and 1000
Å were used for separation. Samples were dissolved in THF using
concentrations of 2–3 mg mL–1. The system
was calibrated using polystyrene standards. Spectra were processed
manually. After baseline correction, data upwards of 202 g mol–1, corresponding to NPG-MA dimers, were used to calculate
the polystyrene equivalents of number and weight average molecular
masses (Mn and Mw) as well as the polydispersity index Đ.
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