Ribonuclease a
Ribonuclease A is a protein-based enzyme that catalyzes the hydrolysis of ribonucleic acid (RNA). It is commonly used in molecular biology and biochemistry laboratories for the degradation and purification of RNA samples.
Lab products found in correlation
6 protocols using ribonuclease a
Cell Cycle Analysis of Transfected MCF-7 Cells
Apoptosis Evaluation of OSCC Cells
Cell Cycle Analysis of Arsenite-Exposed HaCaT Cells
Evaluating Cancer Cell Clonogenicity
To examine cell proliferation, the proliferation index of each group was assessed using a CCK-8 assay (Dojindo Molecular Technologies, Inc., Kumamoto, Japan) as previously described (29 (link)). The proliferation index was calculated as the absorbance detected in the experimental group-the absorbance detected in the blank group.
To analyze the cell cycle, 5×106 cells were fixed with 70% ethanol for 30 min at 4°C. The cell samples were stained with 200 µl propidium iodide (PI; Beyotime Institute of Biotechnology, Haimen, China) in a solution containing ribonuclease A (Beyotime Institute of Biotechnology) for 10 min at room temperature. Subsequently, the samples were analyzed using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA) and FlowJo 7.6.1 (FlowJo LLC, Ashland, OR, USA).
Cell Cycle Analysis of BGC-823 and SGC-7901 Cells
Cell Cycle Analysis of A375/B16 Cells
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