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Mitochondria gfp alexa fluor 488 or 546 labeled secondary antibodies donkey anti mouse igg h l

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Mitochondria-GFP Alexa Fluor 488 or 546 labeled secondary antibodies [donkey anti-mouse IgG (H+L)] are fluorescently-conjugated secondary antibodies designed for the detection and visualization of mouse immunoglobulin G (IgG) proteins. These antibodies are labeled with Alexa Fluor 488 or 546 fluorescent dyes and specifically target the heavy and light chains of mouse IgG. They can be used for immunofluorescence applications to label and detect mouse primary antibodies that have bound to mitochondrial proteins.

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Lab products found in correlation

Dual luciferase kit, by Promega (2 mentions) Anti mrps18b 16139 1 ap, by Proteintech (2 mentions) Anti rpl4 11302 1 ap, by Proteintech (2 mentions) Anti hsp70 spa810, by Enzo Life Sciences (2 mentions) Cleaved caspase 3 9661, by Cell Signaling Technology (2 mentions) Mitotracker, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) β actin a5441, by Merck Group (2 mentions) Puromycin, by Merck Group (2 mentions)

Close spelling variants of this product

Alexa Fluor 488 donkey anti-mouse IgG (H+L) Alexa Fluor 488-labeled donkey anti-mouse IgG Alexa Fluor IgG (H + L) secondary antibodies Alexa Fluor 488 or 546 antibodies Alexa Fluor 546 Donkey Anti-Mouse IgG Alexa-488 labeled donkey anti-mouse IgG Alexa Fluor 488 donkey anti-mouse IgG Alexa Fluor 546 donkey anti-mouse Alexa Fluor 488 IgG secondary antibodies Alexa Fluor 488 or 546

2 protocols using mitochondria gfp alexa fluor 488 or 546 labeled secondary antibodies donkey anti mouse igg h l

1

Regulation of Mitochondrial Ribosome Biogenesis

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HeLa cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS). HSF1+/+ and HSF1−/− MEFs were kindly provided by I. J. Benjamin (University of Utah). Cycloheximide (CHX), poly-U and puromycin were purchased from Sigma. MitoTracker, Mitochondria-GFP Alexa Fluor 488 or 546 labeled secondary antibodies [donkey anti-mouse IgG (H+L)] and Hoechst were purchased from Invitrogen. Anti-MRPL18 (HPA028774), anti-MRPL38 (HPA023054), anti-RPS20 (HPA003570), anti-RPL5 (HPA043717), anti-myc (C3956) and β-actin (A5441) monoclonal antibody was purchased from Sigma; anti-MRPS18B (16139-1-AP) and anti-RPL4 (11302-1-AP) was from Proteintech; anti-Hsp70 (SPA810) was from Enzo Life Sciences; anti-RPS6 (#2217), anti-HSP60 (#4870), anti-Caspase 3 (#9662)and cleaved Caspase 3 (#9661) antibody were from Cell Signaling. Dual luciferase kit was purchased from Promega. siRNA SMARTpools targeting human MRPL18 and control were purchased from Dharmacon. siRNA targeting human Lyn, MRPL38 and MRPS18B were purchase from Santa Cruz Biotechnology. Plasmids and siRNA transfections were performed using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instruction.
Zhang X., Gao X., Coots R.A., Conn C.S., Liu B, & Qian S.B. (2015). Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology, 22(5), 404-410.
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2

Regulation of Mitochondrial Ribosome Biogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS). HSF1+/+ and HSF1−/− MEFs were kindly provided by I. J. Benjamin (University of Utah). Cycloheximide (CHX), poly-U and puromycin were purchased from Sigma. MitoTracker, Mitochondria-GFP Alexa Fluor 488 or 546 labeled secondary antibodies [donkey anti-mouse IgG (H+L)] and Hoechst were purchased from Invitrogen. Anti-MRPL18 (HPA028774), anti-MRPL38 (HPA023054), anti-RPS20 (HPA003570), anti-RPL5 (HPA043717), anti-myc (C3956) and β-actin (A5441) monoclonal antibody was purchased from Sigma; anti-MRPS18B (16139-1-AP) and anti-RPL4 (11302-1-AP) was from Proteintech; anti-Hsp70 (SPA810) was from Enzo Life Sciences; anti-RPS6 (#2217), anti-HSP60 (#4870), anti-Caspase 3 (#9662)and cleaved Caspase 3 (#9661) antibody were from Cell Signaling. Dual luciferase kit was purchased from Promega. siRNA SMARTpools targeting human MRPL18 and control were purchased from Dharmacon. siRNA targeting human Lyn, MRPL38 and MRPS18B were purchase from Santa Cruz Biotechnology. Plasmids and siRNA transfections were performed using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instruction.
Zhang X., Gao X., Coots R.A., Conn C.S., Liu B, & Qian S.B. (2015). Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. Nature structural & molecular biology, 22(5), 404-410.
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