Cxcr4 l276f12

Manufactured by BioLegend

CXCR4 (L276F12) is a recombinant monoclonal antibody that recognizes the chemokine receptor CXCR4. CXCR4 is a G-protein-coupled receptor that plays a key role in cell migration and homing. The antibody is suitable for flow cytometry and other immunoassay applications.

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Lab products found in correlation

Fluorochrome conjugated antibodies, by Thermo Fisher Scientific (1 mentions) Jes6 5h4, by BioLegend (1 mentions) Cd62l mel 14, by BioLegend (1 mentions) Macsquant flow cytometer, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

CXCR4 (9 citations) CXCR4 (clone L276F12) (2 citations) PerCP/Cy5.5 anti-mouse CXCR4 (clone L276F12, (2 citations) 162Dy-CXCR4 (clone L276F12, (2 citations) CXCR4 (L276F12) APC (2 citations)

2 protocols using cxcr4 l276f12

Multiparameter Flow Cytometry Staining

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Fluorochrome-conjugated antibodies against the following surface and intracellular molecules were purchased from eBioscience, BD Biosciences, or BioLegend: CD45.1 (A20), CD45.2 (104), NK1.1 (PK136), CD4 (RM4-5), CD8α (53-6.7), Ter119 (Ter119), CD11b (M1/70), B220 (RA3-6B2), CD44 (IM7), CD62L (MEL-14), CXCR4 (L276F12), IL-2 (JES6-5H4), IFNγ (XMG1.2), granzyme B (GB11), and Eomes (Dan11mag).

Deets K.A., Berkley A.M., Bergsbaken T, & Fink P.J. (2016). Enhanced clonal burst size corrects an otherwise defective memory response by CD8+ recent thymic emigrants. Journal of immunology (Baltimore, Md. : 1950), 196(6), 2450-2455.

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Multiparametric Flow Cytometry of MDSCs

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Surface staining was performed as previously described (33 (link)). Cells were stained with FITC-, PerCP-, PE-, APC-, Pe-Cy7, APC-Cy7-, or Brilliant Violet 421- conjugated antibodies and analyzed by a MACSQuant flow cytometer (Miltenyi Biotec; Auburn, CA) to detect the expression of murine TLR-4 (MTS510), IL-4Rα (CD124) (mIL4R-M1), F4/80 (BM8), FcγRIII/II (93), CD11b (M1/70), Ly6C (HK1.4), GR-1 (RB6-8C5), CXCR4 (L276F12), CXCR2 (SA045E1), CX₃CR1(SA011F11), or CCR2 (475301) (BioLegend; San Diego, CA; BD Biosciences; San Jose, CA; R&D Systems; Minneapolis, MN). Positive gates were selected based on isotype or fluorescence minus one (FMO) controls and data were analyzed using FlowJo software (Tree Star, Inc.). All representative flow cytometry dot plots and subsequent phenotypic analyses are of unsorted M-MDSCs.

O'Connor M.A., Fu W.W., Green K.A, & Green W.R. (2015). Subpopulations of M-MDSCs from mice infected by an immunodeficiency-causing retrovirus and their differential suppression of T- vs B-cell responses. Virology, 485, 263-273.

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