PR-619 was obtained from Merck Millipore (Billerica, MA, USA), cisplatin was obtained from clinical preparations of Abiplatin solution (Pharmachemie BV, GA Haarlem, the Netherlands) and ABT-199 was obtained from Abcam (Cambridge, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Merck Millipore. For Western blot analysis, the antibodies against CHOP (#2895), Bcl-2 (#2827), P21 (#2947), and cleaved-PARP (#5625) were obtained from Cell Signaling Technology (Danvers, MA, USA). The caspase-4 antibody (#M029-3) was purchased from MBL (Woburn, MA, USA); the β-actin (#109639) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, #100118) antibodies were purchased from GeneTex (Irvine, CA, USA); the phospho-CDK2 (Tyr15) and phospho-Histone H3 (Ser10) antibodies were purchased from Abcam (#136810); the ubiquitin antibody (#BML-PW0150) was purchased form Enzo Life Sciences (Farmingdale, NY, USA), and the other antibodies against GRP78 (#sc-13968) and α-tubulin (#sc-5286) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). For immunohistochemistry (IHC), the Bcl-2 antibody (#sc-7382) was obtained from Santa Cruz Biotechnology.
Abt 199
Manufactured by Abcam
Sourced in Netherlands, United Kingdom
ABT-199 is a small molecule compound that selectively inhibits the anti-apoptotic protein BCL-2. It is used in research applications to study apoptosis and cancer biology.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved parp, by Cell Signaling Technology (1 mentions)
Bcl 2 antibody, by Santa Cruz Biotechnology (1 mentions)
Pr 619, by Merck Group (1 mentions)
α tubulin sc 5286, by Santa Cruz Biotechnology (1 mentions)
β actin, by GeneTex (1 mentions)
Jnk in 8, by Merck Group (1 mentions)
Venetoclax, by Abcam (1 mentions)
Navitoclax, by Abcam (1 mentions)
2 protocols using abt 199
1
Apoptosis Pathway Regulation Assay
2
Inhibitor Evaluation in Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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IMT, NRT (nirotinib), and DST (dasatinib) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Cell signaling pathway inhibitors U0126, LY294002, SB203580, and SP600125 were obtained from Calbiochem (San Diego, CA, USA). The BCL2-specific inhibitor ABT-199 (venetoclax), pan-BCL2 family inhibitor ABT-263 (navitoclax), and MCL1 inhibitor A-1210477 were from Abcam (Cambridge, UK). The MCL1 inhibitor marinopyrrole A (moritoclax) was purchased from ChemScene (Monmouth Junction, NJ, USA). The JNK inhibitor JNK-IN-8 was obtained from Sigma (St. Louis, MO, USA).
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