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Smartdish 6 well plates

Manufactured by STEMCELL
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The SmartDish™ 6-well plates are a lab equipment product designed for cell culture applications. The plates feature six individual wells, each with a flat bottom surface suitable for adherent cell growth. The plates are made of tissue culture-treated polystyrene material to provide a consistent surface for cell attachment. The product dimensions and well volume are standardized for common laboratory use.

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Lab products found in correlation

Stemgrid 6 counting grid, by STEMCELL (2 mentions) Methocult gf m3434, by STEMCELL (1 mentions) Stemvision hematopoietic colony counter, by STEMCELL (1 mentions) Methocult h4434 classic, by STEMCELL (1 mentions)

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SmartDish (23 citations) SmartDish plates (6 citations) SmartDishes (2 citations)

4 protocols using smartdish 6 well plates

1

Quantifying Enteric Neurospherogenesis In Vitro

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Dissociated enterocytes were cultivated in SmartDish™ 6-well plates (STEMCELL Technologies, Vancouver, BC, Canada) as described above for 1 week. Then, neurospheres were enumerated under the 10× objective. STEMgrid™-6 counting grid (STEMCELL Technologies) was attached to the bottom of SmartDish™ 6-well plates. Five areas, of which each contained 4 grids of 2 mm × 2 mm, were marked for sphere enumeration. In each well, neurospheres generated on 20 selected grids were counted and classified by their maximal diameter of <50 μm, 50–100 μm, and >100 μm. Enteric neurospherogenesis was defined by the total neurosphere number per unit area (mm2).
Chen J.C., Yang W., Tseng L.Y, & Chang H.L. (2022). Enteric Neural Network Assembly Was Promoted by Basic Fibroblast Growth Factor and Vitamin A but Inhibited by Epidermal Growth Factor. Cells, 11(18), 2841.
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2

Quantifying Enteric Neurosphere Generation

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Dissociated enterocytes were cultivated non-adherently in SmartDish™ 6-well plates (STEMCELL Technologies) at the cell dose of 2 × 105 in NSM or SRM as described above. On day 7, neurospheres generated were counted using an inverted phase-contrast microscope. For neurosphere enumeration, STEMgrid™-6 counting grid (STEMCELL Technologies) was attached to the bottom of SmartDish™ 6-well plates. Five zones, of which each contained 4 grids of 2 × 2 mm, were labeled for neurosphere counting. In each 35-mm well, neurospheres growing on 20 selected grids were enumerated. Enteric neurospherogenesis was defined by neurosphere (≥ 50 μm in diameter) number per unit area (mm2).
Chen J.C., Yang W., Tseng L.Y, & Chang H.L. (2023). Enteric neurospheres retain the capacity to assemble neural networks with motile and metamorphic gliocytes and ganglia. Stem Cell Research & Therapy, 14, 290.
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3

Clonogenic Assay for AML Cells

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FACS-sorted viable AML cells (GFP+7-AAD−) were resuspended in MethoCult GF M3434 (StemCell Technologies) at 1000 cells/ml and plated in SmartDish 6-well plates (StemCell Technologies) at 1 ml/well in duplicate. Cells were cultured in a humidified incubator at 37°C in ambient air with 5% CO2. After 5-days colonies were counted.
van Gastel N., Spinelli J.B., Sharda A., Schajnovitz A., Baryawno N., Rhee C., Oki T., Grace E., Soled H.J., Milosevic J., Sykes D.B., Hsu P.P., Vander Heiden M.G., Vidoudez C., Trauger S.A., Haigis M.C, & Scadden D.T. (2020). Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance. Cell metabolism, 32(3), 391-403.e6.
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4

Colony Formation Assay of HSPCs

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At 2-3 days postelectroporation, HSPCs were plated in SmartDish 6 well plates (catalogue number 27370; STEMCELL Technologies) containing MethoCult H4434 Classic or MethoCult H4434 Classic without EPO (catalogue numbers 04444 and 04544; STEMCELL Technologies). After 14 days, the wells were imaged using the STEMvision Hematopoietic Colony Counter (STEMCELL Technologies). Colonies were counted and scored to determine the number of CFU-GEMM (colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte), CFU-GM (colony-forming unit-granulocyte, macrophage), BFU-E (burst-forming uniterythroid) and CFU-E (colony-forming unit-erythroid) colonies.
Luna S.E., Camarena J., Hampton J.P., Majeti K.R., Charlesworth C.T., Soupene E., Selvaraj S., Jia K., Sheehan V.A., Cromer M.K, & Porteus M.H. (2024). Enhancement of erythropoietic output by Cas9-mediated insertion of a natural variant in haematopoietic stem and progenitor cells. Nature biomedical engineering.
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