Fei talos f200x tem

Following in vitro tau fibril formation, the fibril samples were subjected to sonication for 10 min in a water bath. Prior to sample preparation for TEM, all grids were pre-exposed to UV light for 1 h. A 1 μl aliquot of sonicated fibril sample was loaded onto a carbon-coated, 300 mesh copper grid (Electron Microscopy Sciences, CF300-CU-50) and allowed to be absorbed for 1 min. The excess liquid was then wicked off. Negative staining was carried out by applying 1 μl of 2% uranyl acetate to the grid and incubating for 30 s. The excess stain was wicked off and the grid was subsequently washed twice with a drop of deionized water. The grid was air-dried prior to imaging using a FEI Talos F200X TEM (ThermoFisher Scientific) at the University of Kentucky Electron Microscopy Center.

VLP preparations were negatively stained and examined by transmission electron microscopy (TEM) at the George Washington University Nanofabrication Imaging Center, Washington, DC. Suspended particles (10 μL) were applied to a glow discharged Formvar carbon-coated copper grid and incubated for 1 min. The VLPs were fixed on grids with 4% glutaraldehyde for 5 min followed by water washes. The samples were then stained with 2% aqueous uranyl acetate for 1 min. The grids were examined at 80 kV using an FEI Talos F200X TEM (Thermo Fisher Scientific, Waltham, MA, USA).