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Oxymax open circuit calorimeter system

Manufactured by Columbus Instruments
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The Oxymax Open Circuit Calorimeter System is a laboratory equipment designed to measure oxygen consumption and carbon dioxide production in various research applications. It operates by monitoring the exchange of gases between a test subject and the surrounding environment.

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Lab products found in correlation

Clax software, by Columbus Instruments (4 mentions) Elisa, by ALPCO (1 mentions) Expedata v 1, by Sable Systems (1 mentions) Matlab 2015b, by MathWorks (1 mentions) Clocklab software, by Actimetrics (1 mentions)

8 protocols using oxymax open circuit calorimeter system

1

Metabolic Assessment in Mice

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A Comprehensive Lab Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments) was used to assess oxygen consumption (VO2) and carbon dioxide (VCO2) production of individual mice over a 24‐hr period (one dark and one light cycle) following 18 hr of habituation (LeBrasseur et al., 2009). VO2 and VCO2 values were used to calculate respiratory exchange ratio (RER). Circulating glucose levels and sensitivity were determined following a 6‐hr fast by measuring concentrations using blood glucose monitoring strips (Bayer) before (time 0) and 15, 30, 60, and 120 min after an intraperitoneal bolus of glucose (1.25 g/kg) (Bernardo et al., 2010). Nonfasted circulating insulin levels were determined by enzyme‐linked immunosorbent assay (ELISA; Alpco) from plasma obtained at necropsy.
Schafer M.J., Mazula D.L., Brown A.K., White T.A., Atkinson E., Pearsall V.M., Aversa Z., Verzosa G.C., Smith L.A., Matveyenko A., Miller J.D, & LeBrasseur N.K. (2019). Late‐life time‐restricted feeding and exercise differentially alter healthspan in obesity. Aging Cell, 18(4), e12966.
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2

Metabolic Flexibility Assessment in Mice

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Indirect calorimetry was performed during light and dark cycles to determine the extent to which treatment affected metabolic parameters during conditions of rest (light cycle) and activity (dark cycle). In addition, the effects of treatment on metabolic flexibility were assessed by measuring metabolic parameters under fed conditions (provision of a predominantly carbohydrate-based fuel source) and then fasted conditions (reliance on endogenous lipid-based energy stores). On the day of the experiment, mice were weighed and acclimated overnight. In a subset of eight mice per group, the habitual ambulatory, rearing, and total activity, oxygen consumption (VO2), and carbon dioxide production (VCO2) of individual mice were monitored over a 24h period (12h light/12h dark) using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER) and VO2. RER values were used to determine the basal metabolic rate (in kilocalories per kilogram per hour). Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Zhang Y., Hao J., Tarrago M.G., Warner G.M., Giorgadze N., Wei Q., Huang Y., He K., Chen C., Peclat T.R., White T.A., Ling K., Tchkonia T., Kirkland J.L., Chini E.N, & Hu J. (2021). FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue. Cell reports, 36(5), 109481.
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3

Circadian Rhythms in Mice Metabolism

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Circadian rhythms in feeding were assessed in mice individually housed in Promethion metabolic cages outfitted with a Promethion MM-1 food intake load cell (at 3-mg resolution). Food intake events were exported by ExpeData v1.9.27 (Sable Systems International) and subsequently binned into 10-s or 5-min intervals using MATLAB 2015b (MathWorks, Natick, MA). Foodograms were constructed using ClockLab software (Actimetrics, Wilmette, IL). Corresponding circadian rhythmicity analysis was calculated using χ2 periodogram analysis in ClockLab. Circadian rhythms in activity were assessed in mice individually housed in Promethion metabolic cages outfitted with BXY beambreak activity monitor system to track gross motor activity and analyzed by ExpeData and MATLAB 2015b as noted. Assessment of energy expenditure was performed using the comprehensive laboratory animal monitoring system (equipped with an Oxymax open-circuit calorimeter system, Columbus Instruments, Columbus, OH). Volume of carbon dioxide production (V.CO2), Volume of oxygen uptake (V.O2), and food intake were monitored and energy expenditure (kilocalories per hour per kilogram) was calculated using Oxymax Software V5.40.14 (Columbus Instruments).
Brown M.R., Sen S.K., Mazzone A., Her T.K., Xiong Y., Lee J.H., Javeed N., Colwell C.S., Rakshit K., LeBrasseur N.K., Gaspar-Maia A., Ordog T, & Matveyenko A.V. (2021). Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of β cell function. Science Advances, 7(51), eabg6856.
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4

Monitoring Murine Metabolic Activity

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In a subset of 8–10 mice per group, habitual ambulatory, rearing and total activity, oxygen consumption (VO2) and carbon dioxide production (VCO2) of individual mice were monitored over a 24-h period (12 h of light and 12 h of dark) using a CLAMS equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). Ambulatory, rearing and total activities were summed and analysed for light and dark periods under fed conditions. VO2 and VCO2 values were used to calculate the respiratory exchange ratio and VO2. Respiratory exchange ratio values were used to determine the basal metabolic rate (in kcal h−1 per kg).
Xu Q., Fu Q., Li Z., Liu H., Wang Y., Lin X., He R., Zhang X., Ju Z., Campisi J., Kirkland J.L, & Sun Y. (2021). The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice. Nature Metabolism, 3(12), 1706-1726.
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5

Indirect Calorimetry Monitoring of Murine Metabolism

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Indirect calorimetry was performed during light and dark cycles to determine the extent to which treatment affected metabolic parameters during conditions of rest (light cycle) and activity (dark cycle). On the day of the experiment, mice were weighed and acclimated overnight. In a subset of eight mice per group, the habitual ambulatory, rearing, and total activity, oxygen consumption (VO2), and carbon dioxide production (VCO2) of individual mice were monitored over a 24 h period (12 h light/12 h dark) using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER) and VO2. RER values were used to determine the basal metabolic rate (in kilocalories per kilogram per hour). Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Burr S.D., Chen Y., Hartley C.P., Zhao X, & Liu J. (2023). Replacement of saturated fatty acids with linoleic acid in western diet attenuates atherosclerosis in a mouse model with inducible ablation of hepatic LDL receptor. Scientific Reports, 13, 16832.
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6

Whole-body Energy Metabolism Monitoring

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Whole-body energy metabolism measurements were performed using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments, Columbus, OH, USA). Mouse weights were measured and mice were acclimated for 3 days in a metabolic chamber with food and water under a normal 12-hours light-dark cycle. Oxygen consumption (VO2) and carbon dioxide production (VCO2) were monitored for 24 hours. The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER). RER was used to assess energy source utilization and energy expenditure (EE) normalized by body weight. Data were analyzed using the CLAX software from Columbus Instruments and visualized using an Excel.
Takaoka S., Yanagiya A., Mohamed H.M., Higa R., Abe T., Inoue K.I., Takahashi A., Stoney P, & Yamamoto T. (2021). Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis. iScience, 24(10), 103151.
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7

Comprehensive Metabolic Monitoring in Mice

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Ambulatory activity, food consumption, oxygen consumption (VO2) and carbon dioxide production (VCO2) of individual mice were monitored over a 48-hour period (24 hours fed and 24 hours fasted) using a comprehensive laboratory animal monitoring system equipped with photocells (CLAMS equipped with an Oxymax Open Circuit Calorimeter System; Columbus Instruments). Activity was analyzed for light and dark periods under both fed and fasted conditions. VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER), and VO2 and RER values were used to determine the metabolic rate (kcal/kg/h).
McGee-Lawrence M.E., White T.A., LeBrasseur N.K, & Westendorf J.J. (2015). Conditional deletion of Hdac3 in osteoprogenitor cells attenuates diet-induced systemic metabolic dysfunction. Molecular and cellular endocrinology, 410, 42-51.
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8

Metabolic Flexibility Assessment in Mice

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Indirect calorimetry was performed during light and dark cycles to determine the extent to which treatment affected metabolic parameters during conditions of rest (light cycle) and activity (dark cycle). In addition, the effects of treatment on metabolic flexibility were assessed by measuring metabolic parameters under fed conditions (provision of a predominantly carbohydrate-based fuel source) and then fasted conditions (reliance on endogenous lipid-based energy stores). On the day of the experiment, mice were weighed and acclimated overnight. In a subset of eight mice per group, the habitual ambulatory, rearing, and total activity, oxygen consumption (VO2), and carbon dioxide production (VCO2) of individual mice were monitored over a 24h period (12h light/12h dark) using a Comprehensive Laboratory Animal Monitoring System (CLAMS) equipped with an Oxymax Open Circuit Calorimeter System (Columbus Instruments). The VO2 and VCO2 values were used to calculate the respiratory exchange ratio (RER) and VO2. RER values were used to determine the basal metabolic rate (in kilocalories per kilogram per hour). Data were analyzed using the CLAX software from Columbus Instruments, exported into Excel and plotted in GraphPad.
Zhang Y., Hao J., Tarrago M.G., Warner G.M., Giorgadze N., Wei Q., Huang Y., He K., Chen C., Peclat T.R., White T.A., Ling K., Tchkonia T., Kirkland J.L., Chini E.N, & Hu J. (2021). FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue. Cell reports, 36(5), 109481.
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