Whole cell lysates were obtained as previously described41 (link). Western blot analyses were performed with anti-collagene type I (Rockland), anti-α-SMA, anti-β-actin, anti-PIAS4 (Sigma), anti-PIAS1, anti-PIAS2, anti-PIAS3, anti-RNA polymerase II (Santa Cruz), anti-acetyl lysine (Cell Signaling Tech), anti-SMAD3, and anti-SIRT1 (Abcam) antibodies.
Anti pias3
Manufactured by Santa Cruz Biotechnology
Anti-PIAS3 is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to the PIAS3 protein, which is a member of the protein inhibitor of activated STAT (signal transducer and activator of transcription) family. The primary role of Anti-PIAS3 is to facilitate the detection and study of the PIAS3 protein in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Anti pias2, by Santa Cruz Biotechnology (4 mentions)
Anti sirt1, by Abcam (3 mentions)
Anti pias1, by Santa Cruz Biotechnology (3 mentions)
Anti pias4, by Merck Group (3 mentions)
Anti acetyl lysine, by Cell Signaling Technology (2 mentions)
Anti rna polymerase 2, by Santa Cruz Biotechnology (2 mentions)
Anti p65, by Santa Cruz Biotechnology (2 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti smad3, by Abcam (1 mentions)
Anti α sma, by Merck Group (1 mentions)
Anti collagene type 1, by Rockland Immunochemicals (1 mentions)
Anti smad3, by Cell Signaling Technology (1 mentions)
Anti hic1, by Santa Cruz Biotechnology (1 mentions)
4 protocols using anti pias3
1
Western Blot Analysis of ECM and Signaling
2
ChIP Assays for PIAS and SMAD3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChIP assays were performed essentially as described before42 (link) with anti-PIAS1, anti-PIAS2, anti-PIAS3 (Santa Cruz), anti-PIAS4 (Sigma), anti-SMAD3 (Cell Signaling Tech) or anti-SIRT1 (Abcam). Precipitated genomic DNA was amplified by real-time PCR with primers as previously described43 (link)44 (link).
3
ChIP-seq Protocol for Transcription Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChIP assays were performed essentially as described before [44 (link)] with anti-PIAS1, anti-PIAS2, anti-PIAS3, anti-PIAS4, anti-HIC1, and anti-p65 (Santa Cruz). Precipitated genomic DNA was amplified by real-time PCR with primers as previously described [13 (link), 39 (link), 41 (link)].
4
Evaluating Protein Interactions and Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue and cell lysates were obtained as previously described [43 (link)]. Western blot analyses were performed with anti-PIAS1, anti-PIAS2, anti-PIAS3, anti-p65, anti-RNA polymerase II (Santa Cruz), anti-PIAS4 (Sigma), anti-acetyl lysine (Cell Signaling Tech), and anti-SIRT1 (Abcam) antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!